Effect of solvent on the spectrum of chlorophyll

Seely, G. R.; Jensen, Richard G.

doi:10.1016/0371-1951(65)80095-9

Cited by 258 publications

(92 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This value can be transformed to a specific absorption coefficient of 0.0202 m2 (mg Chl a)-'. The specific absorption can vary (f 15%) depending on the polarity and refractive index of the solvent (Seeley and Jensen 1965). The pigment concentrated in chloroplasts has an average specific coefficient of in vivo absorption of 0.011 m2 (mg Chl a)-' (Table 2).…”

Section: Discussionmentioning

confidence: 99%

Specific in vivo absorption coefficient of chlorophyll a at 675 nm1

Haardt

Maske

1987

Limnology & Oceanography

View full text Add to dashboard Cite

Spectral absorption of phytoplankton from cultures and natural samples was measured by means of an integrating-sphere photometer. Measurements on suspension yielded specific absorption coefficients for healthy phytoplankton cultures in collimated light at 675 nm between 0.007 and 0.0 13 m2 (mg Chl a) I. These values were independent of cell density and cell wall material.Natural samples of Kiel harbor water yielded specific absorption coefficients at 675 nm of 0.009 and 0.017 m2 (mg Chl a)-I. The specific absorption coefficients in suspension were dependent on the optical properties of the individual cells (packaging effect), decreasing (ca. l/r) with increasing cell size (r radius) and increasing chlorophyll a content per cell as predicted by theory, but are significantly lower than theoretically expected. Comparison with published values shows that specific absorption coefficients of cell suspensions are often overestimated, presumably because of methodological problems.

show abstract

Section: Discussionmentioning

confidence: 99%

Specific in vivo absorption coefficient of chlorophyll a at 675 nm1

Haardt

Maske

1987

Limnology & Oceanography

View full text Add to dashboard Cite

show abstract

“…Photosynthetic rates returned to near control levels within 2 d after rewarming (19) Figure 1. Second trifoliolate leaf tissue was collected 2 h after rewarming began on day 23. No zeaxanthin was detected on day 25.…”

Section: Discussionmentioning

confidence: 99%

Chloroplast Ultrastructure, Chlorophyll Fluorescence, and Pigment Composition in Chilling-Stressed Soybeans

Musser¹,

Thomas²,

Wise³

et al. 1984

Plant Physiol.

View full text Add to dashboard Cite

Shoots of 16-day-old soybeans (Glycine max L. Merr. cv Ransom) were chilled to 10°C for 7 days and monitored for visible signs of damage, ultrastructural changes, perturbations in fluorescence of chlorophyll (Chl), and quantitative changes in Chi a and b and associated pigments. Precautions were taken to prevent the confounding effects of water stress. A technique for the separation of lutein and zeaxanthin was developed utilizing a step gradient with the high performance liquid chromatograph. Visible losses in Chl were detectable within the first day of chilling, and regreening did not occur until the shoots were returned to 25°C. Ultrastructurally, unstacking of chloroplast grana occurred, and the envelope membranes developed protrusions. Furthermore, the lipids were altered to the point that the membranes were poorly stabilized by a glutaraldehyde/osmium double-fixation procedure. Chl fluorescence rates were greatly reduced within 2 hours after chilling began and returned to normal only after rewarming. The rapid loss of Chl that occurred during chilling was accompanied by the appearance of zeaxanthin and a decline in violaxanthin. Apparently a zeaxanthin-violaxanthin epoxidation/de-epoxidation cycle was operating. When only the roots were chilled, no substantial changes were detected in ultrastructure, fluorescence rates, or pigment levels.A large number of crop plants originated in the tropics or subtropics and begin to show deleterious responses as the temperature is lowered below 20°C. If there is physiological and structural damage, it is referred to as 'chilling injury.' The phenomenon has been well described (5,12,13).Among chilling sensitive plants such as Gossypium, Paspalum, Phaseolus vulgaris, and Glycine max, the chloroplast is the first of the organelles to show ultrastructural damage from chilling (1,11,29,35). Changes in chloroplast function have been tabulated for five chilling-sensitive species (26) and decline in photoreductive activity is common (10,14). Since changes in photosynthetic electron transfer activity can be monitored fluorometrically in intact leaves (20,26), changes in fluorescence of Chl have the potential for serving as a very early sign of chilling injury. This expectation is tested herein.Aside from the light energy transfer role of the carotenoids in photosynthetic membranes, they are known protectants of Chl against photooxidation. Apparently under chilling conditions, the equilibrium is shifted in the direction of excessive photooxidation (16, 29

show abstract

“…It is usual in spectroscopy of small molecules to use as a reference point the absorption in the absence of environmental influences, i~e., the situation in the gas phase at low pressure. ~!vent effects on chlorophylls have been widely observed, the most extensive examination being that of Seely and Jensen (1965) for Chi a.…”

Section: Solvationmentioning

confidence: 99%

Primary Events and the Trapping of Energy

Sauer¹

1975

Energetics of Photosynthesis

View full text Add to dashboard Cite

Effect of solvent on the spectrum of chlorophyll

Cited by 258 publications

References 18 publications

Specific in vivo absorption coefficient of chlorophyll a at 675 nm1

Specific in vivo absorption coefficient of chlorophyll a at 675 nm1

Chloroplast Ultrastructure, Chlorophyll Fluorescence, and Pigment Composition in Chilling-Stressed Soybeans

Primary Events and the Trapping of Energy

Contact Info

Product

Resources

About