Effect of Solute on Ice-Solution Interfacial Free Energy; Calculation from Measured Homogeneous Nucleation Temperatures

Rasmussen, Don H.; MacKenzie, Alan P.

doi:10.1007/978-1-4615-8681-4_11

Cited by 86 publications

(59 citation statements)

References 18 publications

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“…I t is interesting to note the absolute range of the crystallization temperatures: extrapolating towards O",, DMSO, the homogenous nucleation temperature of pure water ( -38.3"C) is nearly reached. In the presence of solutes, this value is further reduced with a slope about twice that of the liquidus for most low molecular weight substances (Rasmussen & MacKenzie, 1972). Applying this correlation to DMSO gives an approximately linear decrease of the homogeneous nucleation temperature with the additive concentration which is well within the experimental values shown in Fig.…”

Section: Influence Of Cryo-additive Concentrationsupporting

confidence: 82%

Intracellular ice formation: cryomicroscopical observation and calorimetric measurement

Körber

Englich

Rau

1991

Journal of Microscopy

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, differential scanning calorimetry, human lymphocytes, darkening and twitching types of intracellular ice formation, freezing injury, threshold cooling rate, crystallization temperature, influence of the cryo-additive dimethyl sulphoxide, nucleation mechanisms.The formation of ice crystals within biological cells is generally deleterious and results in a severe loss of cellular viability and function. With the aim of circumventing this lethal event, the mechanisms of nucleation and their dependence on governing parameters such as temperature, cooling rate and solute and/or additive concentration, and the correlation with the osmotically induced water transport across the cell membrane were investigated. Quantitative low-temperature light microscopy was used for this purpose as it offers the major advantage of studying the dynamics of the involved processes. T o substantiate further the visual observations of the morphological changes associated with intracellular ice formation, supplementary studies by differential scanning calorimetry (DSC) were performed under comparable conditions to measure the quantity of water actually transformed into the crystalline state due to the evolution of latent heat.Human lymphocytes were used as a biological model cell. In particular it could be shown that the twitching type of intracellular ice formation which is evident but difficult to observe under the cryomicroscope can be attributed to a liquid-solid phase change within the cells as determined by DSC. Good agreement was obtained between the results measured by both techniques with respect to the following dependencies of governing parameters: the fraction of cells exhibiting intracellular ice determined as a function of the cooling rate shows a sharp demarcation zone with an increase from 0 to loo",, at about the same threshold cooling rate. On the other hand, the temperatures at which intracellular ice forms were found to be only weakly dependent on the cooling rate. With respect to the effect of cryo-additive concentration at a fixed value of the cooling rate, the crystallization temperatures were seen to decrease with concentration. T h e D S C results may hence be regarded as a validation of the microscopic observations.

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Section: Influence Of Cryo-additive Concentrationsupporting

confidence: 82%

Intracellular ice formation: cryomicroscopical observation and calorimetric measurement

Körber

Englich

Rau

1991

Journal of Microscopy

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“…In the case of rigid cells that show deep supercooling, the homogeneous nucleation temperature can be related to rTe by the following relationship (Rasmussen and Mackenzie, 1972):…”

Section: Supercooling and Ice Nucleation Ratesmentioning

confidence: 99%

Freezing Characteristics of Rigid Plant Tissues (Development of Cell Tension during Extracellular Freezing)

Rajashekar

Burke

1996

Plant Physiology

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The freezing characteristics and development of cell tension during extracellular freezing were examined i n supercooling stem tissues of riverbank grapes (Vitis riparia) and cold-hardened leaves of live oak (Quercus virginiana) and mountain cranberry (Vaccinium vitis-idaea). Dormant stem xylem and pith tissues of riverbank grapes were resistant to freeze-induced dehydration above the homogeneous nucleation temperature, and they developed cell tension reaching a maximum of 27 MPa. Similarly, extracellular freezing induced cell tension in the leaves of live oak and mountain cranberry. Maximum cell tension in the leaves of live oak was 16.8 MPa and 8.3 MPa i n the leaves of mountain cranberry. Following peak tensions in the leaves, a decline i n the pressure was observed with progressive freezing. The results suggest that resistance t o cell deformation during extracellular freezing due to cell-wall rigidity can lead to reduced cell dehydration and increased cell tension.A relationship to predict freezing behavior in plant tissues based on cell rigidity is presented. Based on cell-water relations and ice nucleation rates, cell-wall rigidity has been shown to effect the freezing characteristics of plant tissues, including freezeinduced dehydration, supercooling, and homogeneous nucleation temperatures.

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“…Although supercooled water freezes at a temperature determined by its nucleation characteristic, it thaws at the melting point of the tissue water. This has been demonstrated in aqueous solutions and living cells (13,14). Therefore, in a supercooled system, the freezing and melting temperatures are not identical.…”

Section: Resultsmentioning

confidence: 95%

Supercooling Characteristics of Isolated Peach Flower Bud Primordia

Rajashekar¹

1989

Plant Physiol.

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The amount of unfrozen water in dormant peach (Prunus persica [L.] Batsch, cv Redhaven) flower buds, isolated primordia, and bud axes was determined during freezing using pulse nuclear magnetic resonance methods. Differential thermal analysis studies were conducted on whole buds and isolated primordia in the presence of ice nucleation. The results showed that some of the water in isolated primordia remained supercooled in the presence of ice nucleation. Although most tissue water froze (57.5%) following ice nucleation at -2.50C, a considerable amount of water was found to supercool. In the presence of ice nucleation, increased hydration of isolated primordia resulted in the elimination of the supercooling characteristic. The structural integrity of isolated primordia appeared to be essential for supercooling.

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Effect of Solute on Ice-Solution Interfacial Free Energy; Calculation from Measured Homogeneous Nucleation Temperatures

Cited by 86 publications

References 18 publications

Intracellular ice formation: cryomicroscopical observation and calorimetric measurement

Intracellular ice formation: cryomicroscopical observation and calorimetric measurement

Freezing Characteristics of Rigid Plant Tissues (Development of Cell Tension during Extracellular Freezing)

Supercooling Characteristics of Isolated Peach Flower Bud Primordia

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