Effect of sodium sulfate on in vitro organogenesis of tobacco callus

Pua, Eng‐Chong; Ragolsky, Elena; Chandler, Stephen F.; Thorpe, Trevor A.

doi:10.1007/bf00033569

Cited by 13 publications

(5 citation statements)

References 21 publications

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“…When tobacco cells are grown on salt they lose water [2], experience an increase in water and osmotic potentials [2,6,15], and accumulate ions and metabolites such as proline and reducing sugars [6,21]. Proliferation of salt tolerant cells, which may also have other phenotypic characteristics, is also favored.…”

Section: Discussionmentioning

confidence: 99%

“…Studies with NaCl-grown callus [2,6,21,22] have not revealed a similar organogenic response. Our physiological studies were undertaken using CP medium, since addition of Na 2SO 4 promotes adventitious shoot initiation [15].…”

Section: Discussionmentioning

confidence: 99%

“…Stock callus cultures were initiated [19] from pith explants of Nicotiana tabacum cv Wisconsin 38 on either SF medium (shoot-forming medium; [14]) or CP medium (callus proliferation medium; [15]) supplemented with 0, 0.75, 1 or 1.5% (w/v; 0, 52.5, 70, 105 mM, respectively)Na2SO4. Cultures maintained without supplements are referred to as control cultures.…”

Section: Initiation and Maintenance Of Culturesmentioning

confidence: 99%

“…We have observed that sodium sulfate promotes shoot formation in tobacco callus. This salt was shown to induce adventitious shoot formation on a medium designed for callus proliferation [15], and enhance shoot formation on a medium supporting caulogenesis [14].…”

Section: Introductionmentioning

confidence: 99%

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Some morphogenic effects of sodium sulfate on tobacco callus

Chandler

Ragolsky

Pua

et al. 1987

Plant Cell Tiss Organ Cult

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Callus cultures of Nicotiana tabacum L cv. Wisconsin 38 were initiated and grown on shoot-forming (SF) and callus proliferation (CP) medium with or without Na2SO 4. Two cultures were maintained on SF medium with 0, 0.75, 1 or 1.5% Na2SO 4 for 2.5 and 3.5 years. In the older culture only callus grown on salt formed shoots throughout the maintenance period, while in the younger culture the control responded best and Na 2 SO 4 was inhibitory. Callus from the older culture which had been grown on salt continued to form shoots in the absence of salt. Na 2 SO 4 caused adventitious shoot formation in three cultures on CP medium. These shoots were present for 7 subcultures after removal of Na2 SO4; but established, control callus, did not form shoots when transferred to Na 2 SOn. Callus initiated and maintained on NaC1 or mannitol showed a slight increase in shoot initiation. On NaC1, Na 2 SO4 or mannitol, the tissue osmotic potential became more negative and proline concentration increased.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Initiation and Maintenance Of Culturesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Some morphogenic effects of sodium sulfate on tobacco callus

Chandler

Ragolsky

Pua

et al. 1987

Plant Cell Tiss Organ Cult

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“…For control I cultures, shoot tips were transferred to 125 cm^ Erlenmeyer flasks each containing 40 cm^ shoot-proliferating (SP) medium described below and subcultured monthly. Shoots arising from callus grown on callus-proliferating medium in the presence of Na2SO4 as previously described (Pua et al, 1985) were also selected and grown on SP medium supplemented with 1 % w/v Na2SO4. These gave rise to plants with different degrees of Na2SO4 tolerance (see Results section).…”

Section: Culture Initiationmentioning

confidence: 99%

Differential Na2SO4 tolerance in tobacco plants regenerated from Na2SO4-grown callus

Pua¹,

Trevor²

1986

Plant Cell Environ

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Abstraet. The regenerated shoots frotn sodium sulphate (Na2SO4) grown callus of tobacco (Nieotiana tabaeum L. cv. Wisconsin 38) were evaluated for Na2SO4 tolerance based on shoot proliferation and rooting //; vitro, and seed germination in vivo in response to Na2SO4. An increase in NajSO^ concentration resulted in significantly decreasing shoot fresh weight, nutnber of shoots, shoot length and leaf size, and increasing per cent shoot dry weight of both control and Na2SO4grown cultures. In rooting, shoots of Na2SO4-grown cultures exhibited the highest per cent rooting (85%) in the presence of 1% w/v Na2SO4. However, per cent rooting, root number per rooted cutting and root fresh weight decreased significantly with increasing Na2SO4 concentration when shoots were transferred to the tnedium in the absence of Na2SO4 for 4-monthly passages. Following acclimatization of the rooted shoots of Na2SO4-grown cultures, phenotypic variation was observed during growth and developtnent. There were 13,2% sterile plants. Fertile plants were sorted into normal (N), tolerant (T), and sensitive (S) categories and the respective percentages of plants were 31.6, 44.7 and 10.5, based on per cent germination, germination velocity index and seedling survival to Na2SO4. The response of N, T and S types to Na2SO4 in subsequent shoot proliferation was similar to that of seed germination.

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