Effect of Sodium Selenite on the Metabolite Profile of Epichloë sp. Mycelia from Festuca sinensis in Solid Culture

Zhou, Lianyu; Lu, Jianwei; Ju, Jianting; Ma, Xuehua

doi:10.1007/s12011-021-03054-w

Cited by 3 publications

(2 citation statements)

References 52 publications

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“…In general, selenium is well tolerated by organisms through bioaccumulating and converting to selenoproteins. The glutathione metabolism pathway plays a significant role in selenium metabolism [ 69 ].…”

Section: Discussionmentioning

confidence: 99%

A Dietary Antioxidant Formulation Ameliorates DNA Damage Caused by γ-Irradiation in Normal Human Bronchial Epithelial Cells In Vitro

et al. 2022

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Antioxidants can be used as radioprotectants to reduce DNA damage due to exposure to radiation that could result in malignancies, including lung cancer. Mortality rates are consistently higher in lung cancer, which is usually diagnosed at later stages of cancer development and progression. In this preliminary study, we examined the potential of an antioxidant formulation (AOX2) to reduce DNA damage using a cell model of human normal bronchial epithelial cells (BEAS-2B). Cells were exposed to γ-irradiation or smoke-related hydrocarbon 4[(acetoxymethyl)nitrosamino]-1 (3-pyridyl) 1-butanone (NNKOAc) to induce DNA damage. We monitored intracellular reactive oxygen species (ROS) levels and evidence of genotoxic damage including DNA fragmentation ELISA, γ-H2AX immunofluorescence, and comet assays. Pre-incubation of the cells with AOX2 before exposure to γ-irradiation and NNKOAc significantly reduced DNA damage. The dietary antioxidant preparation AOX2 significantly reduced the induction of the tumor suppressor protein p53 and DNA damage-associated γ-H2AX phosphorylation by radiation and the NNKOAc treatment. Thus, AOX2 has the potential to act as a chemoprotectant by lowering ROS levels and DNA damage caused by exposure to radiation or chemical carcinogens.

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Section: Discussionmentioning

confidence: 99%

A Dietary Antioxidant Formulation Ameliorates DNA Damage Caused by γ-Irradiation in Normal Human Bronchial Epithelial Cells In Vitro

et al. 2022

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“…Our previous study found that different concentrations of sodium selenite (Na 2 SeO 3 ) induced changes in the growth and metabolite profile of Epichloë sp. mycelia cultivated on potato dextrose agar [31]. The mechanism of fungal survival under Se conditions may be expressed in several ways, among which may be that of alterations in metabolite secretion.…”

Section: Introductionmentioning

confidence: 99%

Effect of Sodium Selenite Concentration and Culture Time on Extracellular and Intracellular Metabolite Profiles of Epichloë sp. Isolated from Festuca sinensis in Liquid Culture

Zhou

Xie

et al. 2022

Agriculture

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Selenium (Se) is not only an essential trace element critical for the proper functioning of an organism, but it is also an abiotic stressor that affects an organism’s growth and metabolite profile. In this study, Epichloë sp. from Festuca sinensis was exposed to increasing concentrations of Na2SeO3 (0, 0.1, and 0.2 mmol/L) in a liquid media for eight weeks. The mycelia and fermentation broth of Epichloë sp. were collected from four to eight weeks of cultivation. The mycelial biomass decreased in response to increased Se concentrations, and biomass accumulation peaked at week five. Using gas chromatography-mass spectrometry (GC-MS), approximately 157 and 197 metabolites were determined in the fermentation broth and mycelia, respectively. Diverse changes in extracellular and intracellular metabolites were observed in Epichloë sp. throughout the cultivation period in Se conditions. Some metabolites accumulated in the fermentation broth, while others decreased after different times of Se exposure compared to the control media. However, some metabolites were present at lower concentrations in the mycelia when cultivated with Se. The changes in metabolites under Se conditions were dynamic over the experimental period and were involved in amino acids, carbohydrates, organic acids, fatty acids, and nucleotides. Based on these results, we conclude that selenite concentrations and culture time influence the growth, extracellular and intracellular metabolite profiles of Epichloë sp. from F. sinensis.

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Effects of the fermentation process on the selenite metabolism and selenium incorporation and speciation in a probiotic Bifidobacterium longum

et al. 2022

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The influence of the fermentation process on selenite metabolism by a probiotic Bifidobacterium longum DD98 (B. longum DD98), and its consequent enrichment in selenium (Se) were studied. The effects of sodium selenite (Na2SeO3) concentration (18−400 μg/mL), feeding time (12, 16, and 24 h), and fermentation stage (secondary and tertiary fermentation) were evaluated by measuring i) the total Se content and its distribution between the water-soluble metabolome fraction and the water-insoluble fraction; ii) the total concentrations of the two principal Se-compounds produced: selenomethionine (SeMet) and γ-glutamyl-selenomethionine (γ-Glu-SeMet), and iii) the speciation of Se in the metabolite fraction. The results revealed that the fermentation process notably changed the Se incorporation into metabolites (γ-Glu-SeMet and free SeMet) and proteins (bound-SeMet) in B. longum DD98. In particular, the production of SeMet was negatively correlated to that of γ-Glu-SeMet when no red precipitate was seen in the bacteria. The study offers a tool for the control of the optimization of the fermentation process towards the desired molecular speciation of the incorporated Se and hence contributes to the production of Se-enriched probiotics with good qualities and bioactivities.

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Effect of Sodium Selenite on the Metabolite Profile of Epichloë sp. Mycelia from Festuca sinensis in Solid Culture

Cited by 3 publications

References 52 publications

A Dietary Antioxidant Formulation Ameliorates DNA Damage Caused by γ-Irradiation in Normal Human Bronchial Epithelial Cells In Vitro

A Dietary Antioxidant Formulation Ameliorates DNA Damage Caused by γ-Irradiation in Normal Human Bronchial Epithelial Cells In Vitro

Effect of Sodium Selenite Concentration and Culture Time on Extracellular and Intracellular Metabolite Profiles of Epichloë sp. Isolated from Festuca sinensis in Liquid Culture

Effects of the fermentation process on the selenite metabolism and selenium incorporation and speciation in a probiotic Bifidobacterium longum

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