Effect of Sodium Molybdate on the Interaction of Androgens and Progestins With Binding Proteins in Human Hyperplastic Prostatic Tissue

Sirett, D.A.N.; Grant, J. K.

doi:10.1677/joe.0.0920095

Cited by 24 publications

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“…In recent studies in which incubations were performed in the presence of molybdate, Kd's for the progesterone binder in cytosols were in the range of 0.2-1.0nM and Bmax's were in 20-90fmol/mg protein (Ekman et al, 1982, Sirett and Grant, 1982, Bashirelahi et al, 1983b, Schneider et al, 1984. In the present experiment, it is revealed that binding parameters for 3H-R 5020 in cytosols are almost identical to those of other reports.…”

Section: Binding Of 3h-ru 27987 In Nucler Extractsupporting

confidence: 87%

Properties of progestin-binding protein in benign hypertrophic human prostate.

et al. 1986

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RU 27987 is a new ligand for progesterone receptor and binds in high affinity to nuclei of target tissues of progesterone.Using this compound, progestin-binding components in the benign hypertrophic human prostate were studied, and compared with those examined with R 5020, a conventional ligand, in the study of progesterone receptor.In cytosols, the binding affinity of RU 27987 was higher than that of R 5020, and the number of maximum binding sites for RU 27987 seemed to be large but correlated well with those of R 5020. The binder for RU 27987 sedimented at 8.6 S, and the binding was specific to progestational steroids, indicating that binding properties of this binder in the cytosols are identical to those for R 5020.Although there was no binding with R 5020 in the nuclear extract, a small amount of specific binding with RU 27987 was detected.However, the cytosol bound with RU 27987 was not retained in DNA Sepharose and no specific binder for RU 27987 in the nuclear extract was observed in a sucrose density gradient centrifugation.From these observations, it was assumed that the nuclear binding observed was attributable to contamination of the cytosolic binder.The results obtained in the present study suggest that the progestinbinding component in the benign prostatic hypertrophy is not the progesterone receptor but a high affinity binder for progestins whose physiological role is not clear at present.

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Section: Binding Of 3h-ru 27987 In Nucler Extractsupporting

confidence: 87%

Properties of progestin-binding protein in benign hypertrophic human prostate.

et al. 1986

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“…Evidence that receptors in many tissues are stabilized by sodium molybdate, thus, Sirett et al 1982, studied the effect of the molybdate which is related to the receptor levels with response to endocrine therapy in prostatic cancer. They demonstrated that 10 mmol l −1 molybdate caused a threefold rise in androgen binding affinity in the cytosol and small progress in the number of progestin-binding sites in the cytosol [7].…”

Section: Western Blottingmentioning

confidence: 99%

“…P53 is a positive regulator of BAX gene expression which can be inhibited the cell cycle or induced the apoptosis in response to DNA damage in the testis [6]. The internal pathway is controlled by BAX and BCL2 genes, which are the members of the bcl2 family Sirett et al, 1982 investigated that in hyperplastic prostatic tissue sodium molybdate affected the response of binding proteins to the androgens [7]. On the other hand, Duan et al 2015 showed that sulfur suppressed the prostate cancer cells proliferation in vivo [8].…”

Section: Introductionmentioning

confidence: 99%

Antiproliferative effect of MoS ₂ in human prostate cancer cell lines

Askari

2019

Biomed. Phys. Eng. Express

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In this investigation, the anti-proliferative activity of a novel molybdenum complex was distinguished on LNCaP (as an androgen-dependent), PC3 (as an androgen-independent) cancer cells, and normal PBD2-fib cells (as a control) using MTT assay, flow cytometry, RT-qPCR, and western blotting. The MoS 2 was prepared by the hydrothermal method, and the synthetic MoS 2 characterized using XRD, EDX, FESEM, HRTEM, and Raman spectroscopies to confirm the success of the synthesis and the unique crystal structure. The cells were treated with different concentrations of MoS 2 (0, 5, 10, 20, 35 and 50 μg ml −1 ) for 24, 48 and 72 h. The obtained results showed that the IC50 values for LNCaP (21.02±0.09 μg ml −1 ) and PC3 (23.03±0.07 μg ml −1 ) were significantly lower than that recorded for normal fibroblast cells (41.56±0.012 μg ml −1 ). Flow cytometry findings demonstrated that the complex is effective in reducing cancer cell viability via apoptosis. RT-qPCR data showed a decrease in BCL2 expression and increases in BAX and P53 gene expression, which were also correlated with the synthetic complex response. The expression of P53 protein increased in LNCaP and PC3 cells after treating with MoS 2 . Also, these data show the anti-tumor properties of synthetic molybdenum complexes in prostate cancer cells. To conclude, the results indicated that the novel design of nanoparticles can be created a new generation of nano-therapeutics strategies in different types of cancer.

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“…Because molybdate has been shown to increase the affinity and/or the number of binding sites [14,15] of steroid hormone receptors, 25 mM sodium molybdate was routinely included in separation and homogenization buffers. In the dog prostate cytosol, molybdate increased the affinity of EBP for 3H-estradiol from 5.4 X lo9 L/ mole to 12.7 x lo9 L/mole without significantly altering the number of binding sites (not shown).…”

Section: Cytosol Binding Of 3h-estradiolmentioning

confidence: 99%

Simplified non‐enzymatic technique for isolation of epithelium and muscle from the dog prostate gland

et al. 1986

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Epithelium and muscle from the normal dog prostate were isolated with a rapid, simple, non-surgical separation (NSS) technique, involving mechanical agitation of organ cubes in a high concentration (30 microM) of EDTA for tissue dispersion, and gentle pressure filtration for recovery of epithelium and muscle. Derivation of this NSS procedure utilized the guinea pig seminal vesicle, since it can be surgically separated (SS) into pure epithelium and muscle to serve as controls for the biochemical viability of NSS prepared tissues. The NSS procedure adopted for use yielded not only pure (greater than 95%) epithelium and muscle from intact seminal vesicle, but also activities of 5 alpha-reductase and concentrations of the cytosol estrophile in each tissue, which were not significantly different from the SS counterparts. In the dog prostate gland, the concentrations of 5 alpha-reductase and the cytosol estrophile in the NSS epithelium were 1.58 and 0.43 of the NSS muscle, respectively.

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Effect of Sodium Molybdate on the Interaction of Androgens and Progestins With Binding Proteins in Human Hyperplastic Prostatic Tissue

Cited by 24 publications

References 0 publications

Properties of progestin-binding protein in benign hypertrophic human prostate.

Properties of progestin-binding protein in benign hypertrophic human prostate.

Antiproliferative effect of MoS ₂ in human prostate cancer cell lines

Simplified non‐enzymatic technique for isolation of epithelium and muscle from the dog prostate gland

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Effect of Sodium Molybdate on the Interaction of Androgens and Progestins With Binding Proteins in Human Hyperplastic Prostatic Tissue

Cited by 24 publications

References 0 publications

Properties of progestin-binding protein in benign hypertrophic human prostate.

Properties of progestin-binding protein in benign hypertrophic human prostate.

Antiproliferative effect of MoS 2 in human prostate cancer cell lines

Simplified non‐enzymatic technique for isolation of epithelium and muscle from the dog prostate gland

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Antiproliferative effect of MoS ₂ in human prostate cancer cell lines