Effect of sodium chloride on Leptospirillum ferriphilum DSM 14647T and Sulfobacillus thermosulfidooxidans DSM 9293T: Growth, iron oxidation activity and bioleaching of sulfidic metal ores

Huynh, Dieu; Giebner, Fabian; Kaschabek, Stefan R.; Rivera-Araya, Javier; Levicán, Gloria; Sand, Wolfgang; Schlömann, Michael

doi:10.1016/j.mineng.2019.04.033

Cited by 15 publications

(14 citation statements)

References 27 publications

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“…The presence of sodium chloride inhibited bioleaching of sphalerite by S. thermosulfidooxidans while bioleaching of CuFeS 2 was not affected (Huynh et al, 2019). To extend our previous work (Huynh et al, 2019), bioleaching of FeS 2 by S. thermosulfidooxidans with elevated NaCl concentrations was investigated. Although bacteria used for the pyrite bioleaching in this present study differed from the study of Gahan et al (2010), our study also found that the presence of chloride in pyrite leaching solutions severely affects the leaching yields.…”

Section: Bioleaching Of Pyrite In the Presence Of Sodium Chloridementioning

confidence: 90%

“…Additionally, albeit several studies have been performed on cell attachment of S. thermosulfidooxidans to sulfides (Sampson et al, 2000;Ghauri et al, 2007;Li et al, 2016), no investigation on the attachment of S. thermosulfidooxidans in the presence of chloride is available. Therefore, this study aims to extend our previous work (Huynh et al, 2019) and investigates if chloride inhibits pyrite bioleaching by S. thermosulfidooxidans. In addition to pyrite bioleaching, this study examines the attachment of S. thermosulfobacillus cells on pyrite surfaces in the presence of chloride.…”

Section: Introductionmentioning

confidence: 99%

“…In the presence of 3 g/L chloride, chalcopyrite leaching by S. thermosulfidooxidans Cutipay was clearly improved (Bobadilla-Fazzini et al, 2014). However, chloride concentrations that proved to be beneficial for bioleaching were very low and the effect of chloride could vary between different metal sulfides (Deveci et al, 2008;Huynh et al, 2019). Therefore, to enable bioleaching in presence of relatively high chloride concentrations and to enable the use of seawater in the mining industry, efforts to search for highly chloride-tolerant leaching microorganisms and studies on bioleaching of metal sulfides in presence of chloride have been made over the past decades (Alexander et al, 1987;Deveci et al, 2008;Gahan et al, 2009;Zammit et al, 2012;Huynh et al, 2017;Norris et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…Having the ability to oxidize Fe 2+ and ISC makes S. thermosulfidooxidans a favorable bacterium for heap bioleaching as oxidation of ISC helps to remove excess sulfur compounds and generate the necessary acidity (Dopson and Lindstrom, 1999;Dopson et al, 2009). An earlier study indicated that a chloride concentration of 200 mM inhibited bioleaching of sphalerite by S. thermosulfidooxidans but not of CuFeS 2 (Huynh et al, 2019). Additionally, albeit several studies have been performed on cell attachment of S. thermosulfidooxidans to sulfides (Sampson et al, 2000;Ghauri et al, 2007;Li et al, 2016), no investigation on the attachment of S. thermosulfidooxidans in the presence of chloride is available.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Sodium Chloride on Pyrite Bioleaching and Initial Attachment by Sulfobacillus thermosulfidooxidans

et al. 2020

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Biomining applies microorganisms to extract valuable metals from usually sulfidic ores. However, acidophilic iron (Fe)-oxidizing bacteria tend to be sensitive to chloride ions which may be present in biomining operations. This study investigates the bioleaching of pyrite (FeS 2), as well as the attachment to FeS 2 by Sulfobacillus thermosulfidooxidans DSM 9293 T in the presence of elevated sodium chloride (NaCl) concentrations. The bacteria were still able to oxidize iron in the presence of up to 0.6M NaCl (35 g/L), and the addition of NaCl in concentrations up to 0.2M (~12 g/L) did not inhibit iron oxidation and growth of S. thermosulfidooxidans in leaching cultures within the first 7 days. However, after approximately 7 days of incubation, ferrous iron (Fe 2+) concentrations were gradually increased in leaching assays with NaCl, indicating that iron oxidation activity over time was reduced in those assays. Although the inhibition by 0.1M NaCl (~6 g/L) of bacterial growth and iron oxidation activity was not evident at the beginning of the experiment, over extended leaching duration NaCl was likely to have an inhibitory effect. Thus, after 36 days of the experiment, bioleaching of FeS 2 with 0.1M NaCl was reduced significantly in comparison to control assays without NaCl. Pyrite dissolution decreased with the increase of NaCl. Nevertheless, pyrite bioleaching by S. thermosulfidooxidans was still possible at NaCl concentrations as high as 0.4M (~23 g/L NaCl). Besides, cell attachment in the presence of different concentrations of NaCl was investigated. Cells of S. thermosulfidooxidans attached heterogeneously on pyrite surfaces regardless of NaCl concentration. Noticeably, bacteria were able to adhere to pyrite surfaces in the presence of NaCl as high as 0.4M. Although NaCl addition inhibited iron oxidation activity and bioleaching of FeS 2 , the presence of 0.2M seemed to enhance bacterial attachment of S. thermosulfidooxidans on pyrite surfaces in comparison to attachment without NaCl.

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Section: Bioleaching Of Pyrite In the Presence Of Sodium Chloridementioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of Sodium Chloride on Pyrite Bioleaching and Initial Attachment by Sulfobacillus thermosulfidooxidans

et al. 2020

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“…The other group of microorganisms, such as sulfur-oxidizing bacteria also has capabilities for extracting metals from their deposits. This group consists of the genus Acidithiobacillus, Acidiphilium, Acidiphilum, Sulfobacillus and Sulfolobus (Ghosh et al 2016;Jang and Valix 2017;Jalali et al 2019;Huynh et al 2019;Retnaningrum and Wilopo 2019). Whereas, heterotrophic bacteria such as Leptospirillum ferriphilum, Pseudomonas sp., Bacillus sp., and Acinetobacter sp were also reported to be capable of solubilizing metals (Ghost and Thavamani et al 2017;Wang et al 2018;Dong et al 2019;Saleh et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Optimization of manganese bioleaching activity and molecular characterization of indigenous heterotrophic bacteria isolated from the sulfuric area

et al. 2019

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Abstract. Prasidya DA, Wilopo W, Warmada IW, Retnaningrum E. 2019. Optimization of manganese bioleaching activity and molecular characterization of indigenous heterotrophic bacteria isolated from the sulfuric area. Biodiversitas 20: 1904-1909. The present research evaluated the manganese bioleaching potency of a heterotrophic bacteria KB3B1. This bacterial strain has been isolated from sulfuric area located at Ungaran, Middle of Java, Indonesia using modified 9K medium by adding of several organic nutrients. The manganese bioleaching activities of the strain was analysed by applying of varying glycine concentrations (0, 5, 10, 15 mg mL-1) with pyrolusite pulp densities of 0.02 g cm-3 on a rotary shaker at 180 rpm for 18 days incubation. Several parameters, including the growth of bacteria, pH values, the concentration of soluble manganese and cyanide, were investigated at the interval of 3 days. Molecular characteristics of the strain were further analyzed based on 16S rDNA gene sequences. After 15 days, the maximum yield of manganese 16.6% was achieved under the addition of 10 mg mL-1 glycine. This maximum extract obtained was followed by the maximum bacterial growth, pH, and cyanide product of the strain. Phylogenetic analysis showed that the strain was closely related with Bacillus niacini EP89. Besides, the average frequencies of guanine and cytosine (G+C) of the strain was in same range as that of the reference bacteria in the GenBank and Bergey's Manual Systematics of Bacteria.

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Chalcopyrite Dissolution: Challenges

Bevilaqua,

Toledo,

Crocco

et al. 2024

Advances in Science, Technology &Amp; Innovation

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Effect of sodium chloride on Leptospirillum ferriphilum DSM 14647T and Sulfobacillus thermosulfidooxidans DSM 9293T: Growth, iron oxidation activity and bioleaching of sulfidic metal ores

Cited by 15 publications

References 27 publications

Effect of Sodium Chloride on Pyrite Bioleaching and Initial Attachment by Sulfobacillus thermosulfidooxidans

Effect of Sodium Chloride on Pyrite Bioleaching and Initial Attachment by Sulfobacillus thermosulfidooxidans

Optimization of manganese bioleaching activity and molecular characterization of indigenous heterotrophic bacteria isolated from the sulfuric area

Chalcopyrite Dissolution: Challenges

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