Effect of Smooth Muscle Cells on the Mechanical Response of Rabbit Carotid Arteries in Culture.

Matsumotο, Takeo; Okumura, Eijiro; Miura, Yoshio; Sato, Masaaki

doi:10.1299/jsmec.42.514

Cited by 5 publications

(2 citation statements)

References 6 publications

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“…Similarly, a 50% decrease in elastic modulus and a 75–100% increase in the cross-sectional area have been observed in isolated tendon fascicles cultured for 1 week 23 , 24 . However, this was not the case in the aorta, in which the initial elastic modulus of rabbit common carotid artery did not deteriorate after 1 week of culture without mechanical loading 25 . Such a drastic response in tendon could be relevant to its high strain sensitivity.…”

Section: Discussionmentioning

confidence: 87%

In situ FRET measurement of cellular tension using conventional confocal laser microscopy in newly established reporter mice expressing actinin tension sensor

Wang,

Maeda,

Tsujimura

et al. 2023

Sci Rep

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FRET-based sensors are utilized for real-time measurements of cellular tension. However, transfection of the sensor gene shows low efficacy and is only effective for a short period. Reporter mice expressing such sensors have been developed, but sensor fluorescence has not been measured successfully using conventional confocal microscopy. Therefore, methods for spatiotemporal measurement of cellular tension in vivo or ex vivo are still limited. We established a reporter mouse line expressing FRET-based actinin tension sensors consisting of EGFP as the donor and mCherry as the acceptor and whose FRET ratio change is observable with confocal microscopy. Tension-induced changes in FRET signals were monitored in the aorta and tail tendon fascicles, as well as aortic smooth muscle cells isolated from these mice. The pattern of FRET changes was distinctive, depending on tissue type. Indeed, aortic smooth muscle cells exhibit different sensitivity to macroscopic tensile strain in situ and in an isolated state. This mouse strain will enable novel types of biomechanical investigations of cell functions in important physiological events.

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Section: Discussionmentioning

confidence: 87%

In situ FRET measurement of cellular tension using conventional confocal laser microscopy in newly established reporter mice expressing actinin tension sensor

Wang,

Maeda,

Tsujimura

et al. 2023

Sci Rep

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“…Wall thickening of the aorta is thought to be driven only by the cellular components in the media, i.e., smooth muscle cells (SMCs) [6]. Recently, Nagayama et al [7] found that chromatin distribution in the nucleus changed signi cantly in response to mechanical stimulations.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Heterogeneous Deformation in the Wall of Rabbit Thoracic Aorta at Microscopic Level

Fan

Wang

Maeda

et al. 2019

ABE

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Aortic wall changes dimensions and mechanical properties in response to mechanical stimulation. As these changes are driven by the cells inside the wall, and their mechanical response has been suggested to exhibit a close correlation with nuclear deformation, it is necessary to study the deformation of the aortic wall at microscopic level. Hence, we obtained 200-µm-thick slices of rabbit thoracic aortas in the circumferential-radial and longitudinal-radial planes, and stretched them in the circumferential and longitudinal directions, respectively, under a microscope. The nuclei of smooth muscle cells (SMCs) were stained with Hoechst33342. Each slice was repeatedly stretched stepwise by 4%, while the uorescence images of the cell nuclei as well as the elastin auto-uorescence were captured at each step. Macroscopic and microscopic stretch ratios were obtained from the uorescence images. Local Green strain was calculated from the change in internuclear distance in a specimen stretched in the circumferential direction. The local tissue strain in the circumferential direction was 0.8 to 2.1 times the macroscopic tissue strain, indicating that the aortic wall deformation was heterogeneous at microscopic level. The shear deformation between adjacent elastic laminas was evident at speci c locations, resulting in a shear strain as large as 10%. We also evaluated the relationship between tissue deformation and nuclear deformation from the change in nuclear shape in the specimen stretched in the circumferential and longitudinal directions. In the circumferential stretch, the strain calculated from the length of the nuclei was less than 70% of the macroscopic strain, suggesting that the nuclei of the SMCs are much stiffer than the cytosolic components. Some nuclei rotated noticeably in response to the stretch, and the average and maximum rotation angle was 5 and 11 , respectively, during the entire stretching process. In the longitudinal stretch, the change in nuclear length was not signi cant, suggesting that mechanical stimulation to the SMCs may be smaller in this direction, as reported previously. The present study shows that the deformations of both the extracellular matrix and cell nuclei are highly heterogeneous, which may have a profound effect on the vascular biology.

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Dependency of deformation of cell nucleus on stretch direction of tissue: Relation to anisotropic response of aortic media to hypertension

Fan

Wang

Kim

et al. 2022

Journal of the Mechanical Behavior of Biomedical Materials

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Effect of Smooth Muscle Cells on the Mechanical Response of Rabbit Carotid Arteries in Culture.

Cited by 5 publications

References 6 publications

In situ FRET measurement of cellular tension using conventional confocal laser microscopy in newly established reporter mice expressing actinin tension sensor

In situ FRET measurement of cellular tension using conventional confocal laser microscopy in newly established reporter mice expressing actinin tension sensor

Analysis of Heterogeneous Deformation in the Wall of Rabbit Thoracic Aorta at Microscopic Level

Dependency of deformation of cell nucleus on stretch direction of tissue: Relation to anisotropic response of aortic media to hypertension

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