Effect of slow freezing on morphology and developmental competence of buffalo (Bubalus bubalis) immature oocytes

Gautam, Sanjeev K.; Verma, Vinod; Singh, Birbal; Palta, P.; Singla, S. K.; Chauhan, M. S.; Manik, R. S.

doi:10.1016/j.anireprosci.2007.03.014

Cited by 12 publications

(11 citation statements)

References 19 publications

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“…PROH and EG are common cryoprotectants used in slow freezing and vitrifucation. In addition, ice crystal formation during slow freezing damages the granulosa cells surrounding the oocyte and oocyte cytoskeleton (Mazur 1990b;Liu et al 2003;Gautam et al 2008).…”

Section: Discussionmentioning

confidence: 99%

“…; Gautam et al . ). Vitrification has been recommended as an alternate cryopreservation technique to overcome these disadvantages.…”

Section: Introductionmentioning

confidence: 97%

“…There is very little toxicity and osmotic damage in this process due to the use of low concentration of cryoprotectants. However, slow freezing has several disadvantages, including ice crystal formation, use of expensive programmed cryo-machines and time consumption (Mazur 1990b;Liu et al 2003;Gautam et al 2008). Vitrification has been recommended as an alternate cryopreservation technique to overcome these disadvantages.…”

Section: Introductionmentioning

confidence: 99%

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Vitrification of mouse preantral follicles versus slow freezing: Morphological and apoptosis evaluation

Taghavi

Valojerdi

Moghadam

et al. 2014

Animal Science Journal

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The aim of this study was evaluation of survivability, maturation rate and apoptotic gene expression of preantral follicles after vitrification and slow freezing technique. Normal mouse preantral follicles were randomly divided into three experimental groups. In the control group, follicles were cultured immediately; in the vitrification and slow freezing groups, follicles were cultured after vitrification-warming and slow freezing-thawing procedures. Follicular viability was assessed by using 0.4% trypan blue, and molecular evaluation of messenger RNA levels of apoptosis-related genes was performed by the semi-quantitative RT-PCR method after 3 h of culture. Oocyte maturation rates were also evaluated on day 14 of culture. Survival and maturation rate in the slow freezing group were significantly lower than those in control and vitrification groups (P ≤ 0.05). Although there was no difference in Survivin expression among the three experimental groups, Bcl-2 expression was significantly lower in the slow freezing group compared to the other groups (P ≤ 0.05). The expression of Bax, P53, Fas and Bax/Bcl-2 ratio in the slow freezing group was significantly higher than control and vitrification groups (P ≤ 0.05). Preantral follicle vitrification seems to be better than slow freezing as seen in the survival, maturation and expression rates of apoptotic gene variants.

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Section: Discussionmentioning

confidence: 99%

“…; Gautam et al . ). Vitrification has been recommended as an alternate cryopreservation technique to overcome these disadvantages.…”

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Vitrification of mouse preantral follicles versus slow freezing: Morphological and apoptosis evaluation

Taghavi

Valojerdi

Moghadam

et al. 2014

Animal Science Journal

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“…In buffalo, limited attempts have been made to cryopreserve immature oocytes by slow freezing (Gautam et al 2008) or vitrification (Dhali et al 2000;Wani et al 2004). To our knowledge, there is no information available on the cryopreservation of mature oocytes by slow freezing, with only one report on the vitrification of mature oocytes (Gasparrini et al 2007) The present study was undertaken to investigate the effects of the type of cryoprotectant and concentration on post-thaw morphology, the nature and extent of injuries and the developmental competence of IVM buffalo oocytes subjected to slow freezing or vitrification.…”

Section: Introductionmentioning

confidence: 99%

Effect of type of cryoprotectant on morphology and developmental competence of in vitro-matured buffalo (Bubalus bubalis) oocytes subjected to slow freezing or vitrification

Gautam

Verma

Palta

et al. 2008

Reprod. Fertil. Dev.

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The present study examined the effects of different cryoprotectants on morphology and developmental competence of in vitro-matured buffalo oocytes after slow freezing or vitrification. After slow freezing in dimethyl sulfoxide (DMSO), ethylene glycol (EG) or 1,2-propanediol (PROH), at 1.0 or 1.5 m each, the proportion of morphologically normal oocytes recovered was significantly higher (P < 0.05) with 1.5 than 1.0 m for all cryoprotectants and was highest (P < 0.05) for 1.5 m DMSO. Following vitrification, the percentage of morphologically normal oocytes recovered was lower (P < 0.01) for 40% EG than for 40% DMSO, 20% EG + 20% DMSO or 20% EG + 20% PROH. The most common damage, irrespective of the cryopreservation method, was loss of cumulus mass. The cleavage rate and the proportion of vitrified-warmed oocytes that developed to morulae/blastocysts were significantly higher (P < 0.01) for 20% EG + 20% DMSO than for the other groups. A higher proportion of oocytes developed to morulae (11.5% v. 4.3%) or blastocysts (5.4% v. 0.6%) after vitrification in 20% EG + 20% DMSO than after slow freezing in 1.5 m DMSO. In conclusion, vitrification was more effective than slow freezing for the cryopreservation of in vitro-matured buffalo oocytes.

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“…The major problem with buffalo oocyte cryopreservation is low survival and/or poor developmental competence. Recent reports have highlighted improvements in the cryopreservation of both OPU-and abattoir-derived oocytes, with morulae and blastocysts obtained following IVF of oocytes cryopreserved using slow-freezing or vitrification, although the embryo production efficiency was lower than that for fresh oocytes (Gautam et al 2008a(Gautam et al , 2008b.…”

Section: Cryopreservation Of Oocytes and Embryosmentioning

confidence: 99%

Reproductive biotechniques in buffaloes (Bubalus bubalis): status, prospects and challenges

Singh

Chauhan

Singla

et al. 2009

Reprod. Fertil. Dev.

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The swamp buffalo holds tremendous potential in the livestock sector in Asian and Mediterranean countries. Current needs are the faster multiplication of superior genotypes and the conservation of endangered buffalo breeds. Recent advances in assisted reproductive technologies, including in vitro embryo production methodologies, offer enormous opportunities to not only improve productivity, but also to use buffaloes to produce novel products for applications to human health and nutrition. The use of molecular genomics will undoubtedly advance these technologies for their large-scale application and resolve the key problems currently associated with advanced reproductive techniques, such as animal cloning, stem cell technology and transgenesis. Preliminary success in the application of modern reproductive technologies warrants further research at the cellular and molecular levels before their commercial exploitation in buffalo breeding programmes.

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Effect of slow freezing on morphology and developmental competence of buffalo (Bubalus bubalis) immature oocytes

Cited by 12 publications

References 19 publications

Vitrification of mouse preantral follicles versus slow freezing: Morphological and apoptosis evaluation

Vitrification of mouse preantral follicles versus slow freezing: Morphological and apoptosis evaluation

Effect of type of cryoprotectant on morphology and developmental competence of in vitro-matured buffalo (Bubalus bubalis) oocytes subjected to slow freezing or vitrification

Reproductive biotechniques in buffaloes (Bubalus bubalis): status, prospects and challenges

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