Effect of Skin Lipid Barrier Formation on Hataedock Treatment with Douchi

Abstract

Hataedock is indigenous treatment of Korean medicine that administers herbal extracts orally to newborn infants for remove the fetal heat. The purpose of this study was to evaluate skin lipid barrier formation effect of Hataedock treatment with Douchi. Methods: We measured the Western blot to observe the expression of protein such as involucrin and loricrin. Moreover, we observed immunohistochemical changes in NC/Nga mice. The 3-week-old NC/Nga mice were divided into 3 groups: the 3-week-old control group (3w-Ctrl), 5-week-old control group (5w-Ctrl), and the Hataedock-treated group (5w-FGT). Only the 5w-FGT group was treated with Douchi at the 3rd week. We identified the changes of the lipid skin barrier and protein differentiation through immunohistochemical changes of involucrin, loricrin, filaggrin and acid sphingomyelinase (ASM) in the stratum corneum. Results: The expression of involucrin and loricrin was increased in the Western blot that was treated with concentration of Douchi extracts. In 5w-FGT group, loricrin-positive reaction was increased by 54.0%, involucrin-positive reaction was increased by 84.0%, filaggrin-positive reaction was increased by 108.0% and ASM-positive reaction was increased by 91.0% in the stratum corneum. Conclusions: These results suggest that Hataedock treatment with Douchi promoted skin lipid barrier formation by promoting differentiation of keratinocytes.

Effect of Hataedock Treatment on Epidermal Structure Maintenance through Intervention in the Endocannabinoid System

“…Our previous studies have shown that HTD mitigates AD development due to fetal heat and controls 2skewed conditions [26,27]. Moreover, our studies showed that lipid barrier formation in the epidermis is increased after the application of HTD [28].…”

Efficacy of Hataedock Treatments for Maintenance and Formation of Lipid Barrier in Obese NC/Nga Mice with Dermatophagoides Farinae-Induced Atopic Dermatitis

“…In previous studies, we demonstrated that HTD treatments reduce the inflammatory response by down-regulating Th2 differentiation 48) . In addition, we demonstrated that HTD treatments maintains and fortifies the lipid barrier 11) . Based on these results, we hypothesize that HTD could attenuate AD symptoms…”

Fundamental Coordination Chemistry, Environmental Chemistry, and Biochemistry of Lead(II)

“…This trend results from mixing of states in lead due to orbital angular momentum, which results in a paramagnetic term in the Ramsey equation (187). Consequently, the 207 Pb resonance shifts downfield as donor atoms are changed from oxygen to nitrogen to sulfur (Table IV) (39,178,181,182,(188)(189)(190). Although the Pb(II) 6s lone pair of electrons is expected to also have a profound effect on the 207 Pb chemical shift, only one report to date addresses this question.…”