Effect of Skin Application of Dorogov’s Antiseptic-Stimulant on Behavioral Reactions in Rats

Piavchenko, Gennadii; Dutta, P. K.; Новикова, Н. С.; Pugach, V. A.; Корнева, Е. А.; Nozdrin, V. I.

doi:10.1007/s10517-017-3877-9

Cited by 2 publications

(1 citation statement)

References 3 publications

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“…Silver impregnation techniques are able to identify only 5-10% of all neurons, while the rest of the cells remain nonimpregnated. In contrast, the Nissl methods allow staining of the abundant number of neurons and also detect a synthetic activity of the cells, which is marked by the amount of chromatophilic substance, i.e., ribosomal ribonucleic acid in the rough endoplasmic reticulum and polyribosomes (Huber, 1966;P'yavchenko et al, 2016;Piavchenko et al, 2017). The combined use of classic Nissl and Golgi methods to stain the nervous tissue has been proposed earlier (Glaser and Van der Loos, 1981;Pilati et al, 2008;Kassem et al, 2013), but these show several serious drawbacks both in methodical and practical aspects.…”

Section: Introductionmentioning

confidence: 99%

A combined use of silver pretreatment and impregnation with consequent Nissl staining for cortex and striatum architectonics study

et al. 2022

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Despite a rapid growth in the application of modern techniques for visualization studies in life sciences, the classical methods of histological examination are yet to be outdated. Herein, we introduce a new approach that involves combining silver nitrate pretreatment and impregnation with consequent Nissl (cresyl violet) staining for cortex and striatum architectonics study on the same microscopy slide. The developed approach of hybrid staining provides a high-quality visualization of cellular and subcellular structures, including impregnated neurons (about 10%), Nissl-stained neurons (all the remaining ones), and astrocytes, as well as chromatophilic substances, nucleoli, and neuropil in paraffin sections. We provide a comparative study of the neuronal architectonics in both the motor cortex and striatum based on the differences in their tinctorial properties. In addition to a comparative study of the neuronal architectonics in both the motor cortex and striatum, the traditional methods to stain the cortex (motor and piriform) and the striatum are considered. The proposed staining approach compiles the routine conventional methods for thin sections, expanding avenues for more advanced examination of neurons, blood–brain barrier components, and fibers both under normal and pathological conditions. One of the main hallmarks of our method is the ability to detect changes in the number of glial cells. The results of astrocyte visualization in the motor cortex obtained by the developed technique agree well with the alternative studies by glial fibrillary acidic protein (GFAP) immunohistochemical reaction. The presented approach of combined staining has great potential in current histological practice, in particular for the evaluation of several neurological disorders in clinical, pre-clinical, or neurobiological animal studies.

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Section: Introductionmentioning

confidence: 99%

A combined use of silver pretreatment and impregnation with consequent Nissl staining for cortex and striatum architectonics study

et al. 2022

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Liquid products of meat and bone meal pyrolysis: comprehensive assessment by chromatographic methods

Логутов

Abdrakhmanov²,

Kulikovsky

et al. 2023

Foods and Raw Materials

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Dorogov’s antiseptic stimulators (fractions 2 and 3) are products of meat and bone meal pyrolysis that are used to treat farm animals. However, there is a lack of detailed information about their chemical composition. We aimed to study individual compositions of organic substances in the water- and oil-soluble condensates of these preparations. Dorogov’s antiseptic stimulators ASD-2F and ASD-3F (Agrovetzashchita, Russia) were used as samples of the water- and oil-soluble condensates of meat and bone meal pyrolysis. Volatile substances were identified by gas chromatography and gas chromatography-mass spectrometry, while amino acids were determined by high-performance liquid chromatography. The initial water-soluble condensate contained ammonium salts, amides of carboxylic acids, N-heterocyclic compounds, hydantoins, amino acids, and dipeptides, with a total content of 8% of the condensate’s weight. Its dehydrated concentrate had almost no ammonium salts and amides of carboxylic acids, but its contents of hydantoins, amino acids, dipeptides, and lowvolatile nitrogen-containing heterocycles were 10–15 times as high as those in the initial condensate. The condensate contained 13 dipeptides and 19 amino acids with a total content of 2.5%. According to gas chromatography-mass spectrometry, the oilsoluble condensate contained over 30% of nitriles; 7–10% of higher and aromatic hydrocarbons, phenols, and amides (with esters); and 1–3% of N-heterocyclic compounds, naphthalenes, pyridines, and dipeptides. The nitrogen-containing heterocycles, as well as dipeptides, were similar to those in the water-soluble condensate. We identified 80% of individual organic substances in the water-soluble pyrolytic condensate. Together with its concentrate, they contained more than 220 organic substances divided into 10 main groups. The oil-soluble condensate consisted of over 350 individual organic compounds. The full composition of the preparations can be further identified by three-quadrupole liquid mass spectrometry.

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Effect of Skin Application of Dorogov’s Antiseptic-Stimulant on Behavioral Reactions in Rats

Cited by 2 publications

References 3 publications

A combined use of silver pretreatment and impregnation with consequent Nissl staining for cortex and striatum architectonics study

A combined use of silver pretreatment and impregnation with consequent Nissl staining for cortex and striatum architectonics study

Liquid products of meat and bone meal pyrolysis: comprehensive assessment by chromatographic methods

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