Effect of Single Growth Factor and Growth Factor Combinations on Differentiation of Neural Stem Cells

Choi, Kyung Chul; Yoo, Do Sung; Cho, Kyung Sock; Huh, Pil Woo; Kim, Dal Soo; Park, Chun Kun

doi:10.3340/jkns.2008.44.6.375

Cited by 48 publications

(35 citation statements)

References 38 publications

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“…Three months after transplantation, the immunohistochemical analysis demonstrated that the control-NSCs only distributed at the ONL while BDNFNSCs appeared at the deeper retina of INL, and the graft cells seemed to form synaptic connections with host cells in the retina, which suggested that exogenous BDNF facilitated the migration of transplanted cells. Furthermore, the transplanted BDNF-NSCs differentiated into neurons more effi ciently than the control-NSCs in vivo, which was in accordance with the observation that BDNF can help NSCs to differentiate into neurons in vitro [30] .…”

Section: Wwwchinapharcom Zhou Xm Et Alsupporting

confidence: 73%

A rat model for studying neural stem cell transplantation

et al. 2009

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Aim: The goal of this project was to develop a rat model for neural stem cell (NSC) transplantation studies in which NSCs were modifi ed with brain-derived neurotrophic factor (BDNF) genes that may permit extensive and reliable analysis of the transplants. Methods: NSCs were cultured and purifi ed by limiting dilution assay in vitro and infected with recombinant retrovirus pLXSN-BDNF (BDNF-NSCs) and retrovirus pLXSN (p-NSCs). The expression of BDNF genes in transgenic and control NSC groups was measured by FQ-PCR and ELISA assays. NSCs were then transplanted into the subretinal space of normal rat retinas in four groups, which included NSCs alone, BDNF-NSCs, phosphate buffered saline (PBS) control, and normal control. Survival, migration, and differentiation of donor cells in host retinas were observed with optical coherence tomography (OCT), Heidelberg retina angiograph (HRA), and immunohistochemistry, respectively. Results: The results obtained by FQ-PCR demonstrated that the copy numbers of BDNF gene templates from BDNF-NSCs were the highest among the four groups (P<0.05). Consistent with the results of FQ-PCR, BDNF protein level from the supernatant of the BDNFNSCs group was much higher than that of the other two groups (P<0.05) as suggested by the ELISA assays. HRA and OCT showed that graft cells could successfully survive. Immunohistochemical analysis revealed that transplanted BDNF-NSCs could migrate in the host retinas and differentiate into glial cells and neurons three months after transplantation. Conclusion: BDNF promotes NSCs to migrate and differentiate into neural cells in the normal host retinas.

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Section: Wwwchinapharcom Zhou Xm Et Alsupporting

confidence: 73%

A rat model for studying neural stem cell transplantation

et al. 2009

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“…As the first discovered neurotrophic factor, NGF is one of the most important biological molecules, and is indispensable in the development and function of the nervous system (Zhang et al, 2011). NGF also promotes NSCs to differentiate into neurons and astrocytes (Lachyankar et al, 1997;Levenberg et al, 2005;Nakajima et al, 2007;Choi et al, 2008), a process brought about by the down-regulation of ATF5 expression and upregulation of TIMP-2 (Angelastro et al, 2003;Jaworski and Pérez-Martínez, 2006). Our study demonstrated that NGF failed to promote NSC proliferation based on cell counts and MTT assays, but clearly induced NSC differentiation, manifested as upregulated levels of both GFAP and NSE proteins at days 4 and 7, consistent with previous reports (Lachyankar et al, 1997;Levenberg et al, 2005;Nakajima et al, 2007;Choi et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Effects of ganglioside GM1 and neural growth factor on neural stem cell proliferation and differentiation

Wang

Song

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Neurogenesis, recovery from nerve injury, neurodegeneration, and Parkinson's disease affect people's health, yet the underlying molecular mechanisms remain elusive. Here, we investigated the effect of ganglioside GM1 and neural growth factor (NGF) on neural stem cell (NSC) proliferation and differentiation in vitro to provide a scientific basis for comprehensive treatment of nervous system diseases via NSC application. As widely applied methods of relatively high accuracy, cell counts and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assays were used to assess NSC proliferation. In addition, western blotting 2 Q. Wang et al. Genetics and Molecular Research 15 (3): gmr.15038376 was employed to determine NSC differentiation. Cell counts and MTT assays demonstrated that in epidermal growth factor (EGF)-and basic fibroblast growth factor (bFGF)-containing medium, a high concentration of GM1, but not NGF, significantly elevated NSC proliferation. In NSC cultures lacking EGF and bFGF, cell counts and MTT values were significantly increased compared to those in the negative control group on days 4, 7, and 10 after GM1 (25, 100, and 200 ng/mL) but not NGF (25, 50, 100, and 200 ng/mL) treatment. Western blotting revealed significantly increased expression of nestin (an NSC marker) in NSCs treated with GM1, and upregulation of glial fibrillary acidic protein (a glial cell marker) and neuron-specific enolase (a neuron marker) in those administered NGF. Our results suggest that GM1 and NGF induce NSC proliferation and differentiation, respectively, in a dose-dependent manner.

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“…The bFGF is an important factor for preserving the proliferative multipotency of stem cells, as well as stimulating neuronal precursor cells into neurons or astrocytes. [39,61,62] And also, NGF is recognized to induce neurogenic differentiation and enhance nerve regeneration. [36,37] As expected, the heparin-incorporated microspheres showed a significantly greater loading of both bFGF and NGF than the microspheres without heparin binding.…”

Section: Discussionmentioning

confidence: 99%

Dual growth factor-immobilized microspheres for tissue reinnervation:in vitroand preliminaryin vivostudies

Kim

et al. 2015

Journal of Biomaterials Science, Polymer Edition

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Growth factors (GFs) (basic fibroblast growth factor (bFGF) and/or nerve growth factor (NGF))-immobilized polycaprolactone (PCL)/Pluronic F127 microspheres were prepared using an isolated particulate-melting method and the sequential binding of heparin and GFs onto the microspheres. The GFs immobilized on the microspheres were released in a sustained manner over 28 days, regardless of GF type. From the in vitro culture of muscle-derived stem cells, it was observed that the NGF-immobilized microspheres induced more neurogenic differentiation than the bFGF-immobilized microspheres, as evidenced by a quantitative real-time polymerase chain reaction using specific neurogenic markers (Nestin, GFAP, β-tubulin, and MAP2) and Western blot (Nestin and β-tubulin) analyses. The dual bFGF/NGF-immobilized microspheres showed better neurogenic differentiation than the microspheres immobilized with single bFGF or NGF. From the preliminary animal study, the dual bFGF/NGF-immobilized microsphere group also showed effective nerve regeneration, as evaluated by immunocytochemistry using a marker - β-tubulin. The dual bFGF/NGF-immobilized PCL/Pluronic F127 microspheres may be a promising candidate for nerve regeneration in certain target tissues (i.e. muscles) leading to sufficient reinnervation.

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Effect of Single Growth Factor and Growth Factor Combinations on Differentiation of Neural Stem Cells

Cited by 48 publications

References 38 publications

A rat model for studying neural stem cell transplantation

A rat model for studying neural stem cell transplantation

Effects of ganglioside GM1 and neural growth factor on neural stem cell proliferation and differentiation

Dual growth factor-immobilized microspheres for tissue reinnervation:in vitroand preliminaryin vivostudies

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