Effect of simultaneous down-regulation of pectate lyase and endo-β-1,4-glucanase genes on strawberry fruit softening

Youssef, Sabry M.; Amaya, Iraida; López-Aranda, José M.; Sesmero, Rafael; Valpuesta, Victoriano; Casadoro, Giorgio; Blanco‐Portales, Rosario; Pliego‐Alfaro, Fernando; Quesada, Miguel A.; Mercado, José A.

doi:10.1007/s11032-012-9791-y

Cited by 25 publications

(16 citation statements)

References 31 publications

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“…0.05) of expression levels related to time 0 h in a Kruskal-Wallis test. [Youssef et al, 2013]) had transcripts that continuously decreased during the morning (Supplemental Fig. S7C).…”

Section: A Transcriptome Analysis Suggests Possible Contributions Of mentioning

confidence: 99%

A Hydraulic Model Is Compatible with Rapid Changes in Leaf Elongation under Fluctuating Evaporative Demand and Soil Water Status

et al. 2014

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Plants are constantly facing rapid changes in evaporative demand and soil water content, which affect their water status and growth. In apparent contradiction to a hydraulic hypothesis, leaf elongation rate (LER) declined in the morning and recovered upon soil rehydration considerably quicker than transpiration rate and leaf water potential (typical half-times of 30 min versus 1–2 h). The morning decline of LER began at very low light and transpiration and closely followed the stomatal opening of leaves receiving direct light, which represent a small fraction of leaf area. A simulation model in maize (Zea mays) suggests that these findings are still compatible with a hydraulic hypothesis. The small water flux linked to stomatal aperture would be sufficient to decrease water potentials of the xylem and growing tissues, thereby causing a rapid decline of simulated LER, while the simulated water potential of mature tissues declines more slowly due to a high hydraulic capacitance. The model also captured growth patterns in the evening or upon soil rehydration. Changes in plant hydraulic conductance partly counteracted those of transpiration. Root hydraulic conductivity increased continuously in the morning, consistent with the transcript abundance of Zea maize Plasma Membrane Intrinsic Protein aquaporins. Transgenic lines underproducing abscisic acid, with lower hydraulic conductivity and higher stomatal conductance, had a LER declining more rapidly than wild-type plants. Whole-genome transcriptome and phosphoproteome analyses suggested that the hydraulic processes proposed here might be associated with other rapidly occurring mechanisms. Overall, the mechanisms and model presented here may be an essential component of drought tolerance in naturally fluctuating evaporative demand and soil moisture.

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“…0.05) of expression levels related to time 0 h in a Kruskal-Wallis test. [Youssef et al, 2013]) had transcripts that continuously decreased during the morning (Supplemental Fig. S7C).…”

Section: A Transcriptome Analysis Suggests Possible Contributions Of mentioning

confidence: 99%

A Hydraulic Model Is Compatible with Rapid Changes in Leaf Elongation under Fluctuating Evaporative Demand and Soil Water Status

et al. 2014

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“…However, the minor effect of PG silencing on tomato softening led to the view that PG-mediated pectin disassembly during ripening makes only a small contribution to fruit softening (Brummell & Harpster, 2001;Hadfield & Bennett, 1998). More recent studies on strawberry, apple and papaya have challenged this hypothesis, suggesting a key role for pectin modifications in fruit softening (Atkinson et al, 2012;Fabi et al, 2014;Jiménez-Bermúdez et al, 2002;Quesada et al, 2009;Youssef et al, 2009Youssef et al, , 2013.…”

Section: Introductionmentioning

confidence: 99%

The nanostructural characterization of strawberry pectins in pectate lyase or polygalacturonase silenced fruits elucidates their role in softening

Posé

Kirby

Paniagua

et al. 2015

Carbohydrate Polymers

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“…However, the transgenic manipulation of ripening-related genes encoding pectin-degrading enzymes has challenged this assumption. Thus, the silencing of a pectate lyase gene, an enzyme that catalyses the cleavage of the unesterified galacturonosyl linkage via a β-elimination reaction, significantly increased fruit firmness and reduced post-harvest softening (Jiménez-Bermúdez et al , 2002; Youssef et al , 2009, 2013). This firmer genotype correlated not only with a lower pectin solubilization but also with a decreased depolymerization of covalently bound pectins that were extracted after treating the cell walls with sodium carbonate (Santiago-Doménech et al , 2008).…”

Section: Introductionmentioning

confidence: 99%

Insights into the effects of polygalacturonase FaPG1 gene silencing on pectin matrix disassembly, enhanced tissue integrity, and firmness in ripe strawberry fruits

Posé

Paniagua

Cifuentes

et al. 2013

Journal of Experimental Botany

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Antisense-mediated down-regulation of the fruit-specific polygalacturonase (PG) gene FaPG1 in strawberries (Fragaria×ananassa Duch.) has been previously demonstrated to reduce fruit softening and to extend post-harvest shelf life, despite the low PG activity detected in this fruit. The improved fruit traits were suggested to be attributable to a reduced cell wall disassembly due to FaPG1 silencing. This research provides empirical evidence that supports this assumption at the biochemical, cellular, and tissue levels. Cell wall modifications of two independent transgenic antisense lines that demonstrated a >90% reduction in FaPG1 transcript levels were analysed. Sequential extraction of cell wall fractions from control and ripe fruits exhibited a 42% decrease in pectin solubilization in transgenic fruits. A detailed chromatographic analysis of the gel filtration pectin profiles of the different cell wall fractions revealed a diminished depolymerization of the more tightly bound pectins in transgenic fruits, which were solubilized with both a chelating agent and sodium carbonate. The cell wall extracts from antisense FaPG1 fruits also displayed less severe in vitro swelling. A histological analysis revealed more extended cell–cell adhesion areas and an enhanced tissue integrity in transgenic ripe fruits. An immunohistological analysis of fruit sections using the JIM5 antibody against low methyl-esterified pectins demonstrated a higher labelling in transgenic fruit sections, whereas minor differences were observed with JIM7, an antibody that recognizes highly methyl-esterified pectins. These results support that the increased firmness of transgenic antisense FaPG1 strawberry fruits is predominantly due to a decrease in pectin solubilization and depolymerization that correlates with more tightly attached cell wall-bound pectins. This limited disassembly in the transgenic lines indicates that these pectin fractions could play a key role in tissue integrity maintenance that results in firmer ripe fruit.

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Effect of simultaneous down-regulation of pectate lyase and endo-β-1,4-glucanase genes on strawberry fruit softening

Cited by 25 publications

References 31 publications

A Hydraulic Model Is Compatible with Rapid Changes in Leaf Elongation under Fluctuating Evaporative Demand and Soil Water Status

A Hydraulic Model Is Compatible with Rapid Changes in Leaf Elongation under Fluctuating Evaporative Demand and Soil Water Status

The nanostructural characterization of strawberry pectins in pectate lyase or polygalacturonase silenced fruits elucidates their role in softening

Insights into the effects of polygalacturonase FaPG1 gene silencing on pectin matrix disassembly, enhanced tissue integrity, and firmness in ripe strawberry fruits

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