Effect of Silk Fibroin on Neuroregeneration After Traumatic Brain Injury

Moisenovich, Mikhail M.; Plotnikov, Egor Y.; Moysenovich, Anastasia M.; Silachev, D. N.; Danilina, Tatyana I.; Savchenko, E. S.; Bobrova, M. M.; Safonova, L. A.; Tatarskiy, Victor V.; Котлярова, М. С.; Агапов, И. И.; Zorov, Dmitry B.

doi:10.1007/s11064-018-2691-8

Cited by 22 publications

(14 citation statements)

References 59 publications

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“…We agree with other groups suggesting using a cell carrier matrix to avoid immediate host rejection [26,28]. In addition to hydrogels , silk fibroin biomaterials , chitosan-gelatin-agarose or poly-caprolactone, an autologous fibrin matrix is a good candidate for cell transplantations [34,[69][70][71][72][73][74][75][76][77][78][79][80]. In this study, we evaluated the loading of a plasma-clot matrix with the purified OSCs for future treatment of SCI.…”

Section: Discussionsupporting

confidence: 57%

Olfactory Stem Cells for the Treatment of Spinal Cord Injury: Isolation, Purification and Behaviour in a Plasma Clot Matrix

Rövekamp¹,

Volkenstein²,

Minovi³

et al. 2020

RGM

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Cell therapies represent promising strategies to improve neurological functions after spinal cord injury (SCI). Olfactory mucosa (OM) might be an attractive source of multipotent cells for neuroregeneration because olfactory stem cells (OSCs) are resident. The regenerative capacity of OSCs has been demonstrated in animal models and some clinical case reports. Up to now, there are no standard methods for purification, characterization, and delivery of OSCs to the injury site. However, purification and characterization of the grafted cells are prerequisites for clinical use to ensure maximum safety for the patients. In this study, we isolated and purified OSCs from human OM using the neurosphere assay. Subsequently, the cells were characterized, and the behavior of purified OSCs in a plasma clot was investigated. Our study demonstrated that isolated cells from OM form neurospheres, which cells are positive for CD105 (98%) and CD90 (99%) and negative for Epcam (<1%) and MUC5AC (<1%). Purified OSCs were positive for Nestin, CD44 as well as GFAP and showed a lack of CD34 and CD45 expression. OSCs differentiated into neuron-like cells expressing ß-III tubulin. However, differentiation into adipocytes, chondrocytes or osteoblast could not be observed. In addition, OSCs stayed viable and were able to proliferate within the plasma clot. These results highlight OSC as a candidate for autologous transplantation in combination with the plasma clot as a cell carrier in SCI and neurodegenerative disease.

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Section: Discussionsupporting

confidence: 57%

Olfactory Stem Cells for the Treatment of Spinal Cord Injury: Isolation, Purification and Behaviour in a Plasma Clot Matrix

Rövekamp¹,

Volkenstein²,

Minovi³

et al. 2020

RGM

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“…The in vivo therapeutics of SF were inspected by Moisenvich et al (2019). In a traumatic brain injury model in rats, they found that SF neuroprotected and restored the neurological status up to 25% after 4 days of injection, supporting the growth of primary neuronal cells and astrocytes without toxicity [110]. After cerebral ischemia, the silk hydrolysate showed a positive effect on visual and verbal memory [111].…”

Section: Silk Fibroin To Revert Pathological Conditions Of Strokementioning

confidence: 99%

“…The potential of SF microparticles as a biocompatible regenerative construct has been discussed and assessed by Moisenovich et al (2019). They found that the delivery of SF and SF/gelatin microparticles did not cause any toxicity or activation of microglia and astrocytes.…”

Section: Growth Factors and Drug Deliverymentioning

confidence: 99%

Silk Fibroin: An Ancient Material for Repairing the Injured Nervous System

et al. 2021

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Silk refers to a family of natural fibers spun by several species of invertebrates such as spiders and silkworms. In particular, silkworm silk, the silk spun by Bombyx mori larvae, has been primarily used in the textile industry and in clinical settings as a main component of sutures for tissue repairing and wound ligation. The biocompatibility, remarkable mechanical performance, controllable degradation, and the possibility of producing silk-based materials in several formats, have laid the basic principles that have triggered and extended the use of this material in regenerative medicine. The field of neural soft tissue engineering is not an exception, as it has taken advantage of the properties of silk to promote neuronal growth and nerve guidance. In addition, silk has notable intrinsic properties and the by-products derived from its degradation show anti-inflammatory and antioxidant properties. Finally, this material can be employed for the controlled release of factors and drugs, as well as for the encapsulation and implantation of exogenous stem and progenitor cells with therapeutic capacity. In this article, we review the state of the art on manufacturing methodologies and properties of fiber-based and non-fiber-based formats, as well as the application of silk-based biomaterials to neuroprotect and regenerate the damaged nervous system. We review previous studies that strategically have used silk to enhance therapeutics dealing with highly prevalent central and peripheral disorders such as stroke, Alzheimer’s disease, Parkinson’s disease, and peripheral trauma. Finally, we discuss previous research focused on the modification of this biomaterial, through biofunctionalization techniques and/or the creation of novel composite formulations, that aim to transform silk, beyond its natural performance, into more efficient silk-based-polymers towards the clinical arena of neuroprotection and regeneration in nervous system diseases.

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“…Biomaterials based on silkworm fibroin are able to maintain the adhesion of neurons without adversely affecting their proliferation, morphology, and migration (Qu et al, 2010;Wei et al, 2013). In addition, fibroin scaffolds not only can support the adhesion and viability of cortical neurons but also carry a neuroprotective role demonstrated in the oxygen-glucose deprivation model (Moisenovich et al, 2019). It is known that the inclusion of RGD, GFPGER, and other peptide sequences in the structure of the material that mimic cell-binding motives of fibronectin, collagen, laminin, and other extracellular matrix proteins can regulate cell adhesion and, as a result, neuronal growth (Qu et al, 2010).…”

Section: Discussionmentioning

confidence: 99%