Effect of silk fibroin molecular weight on physical property of silk hydrogel

Kim, H.H.; Song, Dae Woong; Kim, M.J.; Ryu, Su Jung; Um, In Chul; Ki, Chang Seok; Park, Y.H.

doi:10.1016/j.polymer.2016.02.054

Cited by 66 publications

(52 citation statements)

References 32 publications

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“…Figure a summarizes the general procedures of different Mws of SF involved in this study. The regenerated SF solution could be prepared by removing the silk sericin protein and subsequently dissolving in different denaturant solvents . The average Mw of SF treated with LiBr at 25°C for 360 min was determined to be ~176 kDa by GPC (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…SF, which is synthesized by an enormous variety of insect and spider species, has received attentions as potential biomedical purposes in the field of tissue engineering and drug delivery system since of its impressive mechanical strength, low inflammation reaction, and degradation properties . Normally, the molecular weight (Mw) is an important factor that affects the performance of the scaffolds' mechanical properties, degradation behavior and other characteristics in tissue engineering. The SF with controlled degradation rate makes it advantageous for developing the next generation scaffolds degraded gradually along with the tissue regeneration .…”

Section: Introductionmentioning

confidence: 99%

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Tailoring degradation rates of silk fibroin scaffolds for tissue engineering

Zhang

Liu

et al. 2018

J Biomedical Materials Res

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In tissue regenerative medicine, developing tunable degradation rate of biomaterials for predictive functional outcomes remains critical. The implanted scaffolds should degrade gradually along with the tissue regeneration, and the optimal degradation rate of scaffold depends on the tissue type to be regenerated. Herein, the tunable degradation rates of silk fibroin (SF) scaffolds were fabricated through controlling dissolution, hydrolyzing conditions, and freeze-drying. The pore size, water adsorption capacity, and mechanical properties of scaffolds were associated with their average molecular weights. Moreover, in vitro cytotoxicity tests demonstrated that rapid degradation of SF scaffolds would facilitate the Schwann cells proliferation. Furthermore, in vitro enzymatic degradation and in vivo subcutaneous implantation experiments illustrated that SF scaffolds degradation behaviors could be well regulated. Immunohistochemistry staining experiments suggested that SF scaffold-degradation products could promote the endothelial cells proliferation. These results indicate that SF tunable degradation rates are promising candidates in regenerative medicine.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Tailoring degradation rates of silk fibroin scaffolds for tissue engineering

Zhang

Liu

et al. 2018

J Biomedical Materials Res

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“…Additionally, physical properties such as shear elastic modulus varied under different molecular weights of SF. In general, due to their relatively loose network structures, the shear elastic modulus of SF hydrogels decreased with use of smaller molecular weights [82]. SF/nanohydroxyapatite (nanoHA) composite hydrogel was prepared by adding ethanol as a gelling agent; this hydrogel exhibited an interconnected porous structure.…”

Section: Structure Design Of Silk Fibroin-based Biomaterialsmentioning

confidence: 99%

A Review of Structure Construction of Silk Fibroin Biomaterials from Single Structures to Multi-Level Structures

Wang

Wei

et al. 2017

IJMS

381

241

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The biological performance of artificial biomaterials is closely related to their structure characteristics. Cell adhesion, migration, proliferation, and differentiation are all strongly affected by the different scale structures of biomaterials. Silk fibroin (SF), extracted mainly from silkworms, has become a popular biomaterial due to its excellent biocompatibility, exceptional mechanical properties, tunable degradation, ease of processing, and sufficient supply. As a material with excellent processability, SF can be processed into various forms with different structures, including particulate, fiber, film, and three-dimensional (3D) porous scaffolds. This review discusses and summarizes the various constructions of SF-based materials, from single structures to multi-level structures, and their applications. In combination with single structures, new techniques for creating special multi-level structures of SF-based materials, such as micropatterning and 3D-printing, are also briefly addressed.

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“…While the degummed silk fibroin was insoluble in formic acid, the regenerated SF was readily soluble in formic acid [28]. The degummed SF was dissolved in a ternary solvent system of calcium chloride/ethanol/water (1:2:8 in molar ratio) at 85 °C for 4 h. After dialysis with a cellulose tubular membrane (molecular cut-off, 12,000) in distilled water for three days, the aqueous SF solution was filtered and freeze-dried to obtain regenerated SF sponges [29,30]. The SF solution was prepared by dissolving the regenerated SF sponges in formic acid for 30 min [28].…”

Section: Methodsmentioning

confidence: 99%

Gelation Behaviors and Mechanism of Silk Fibroin According to the Addition of Nitrate Salts

Kim

Yoon

et al. 2016

IJMS

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Silk fibroin (SF) is a typical fibrous protein that is secreted by silkworms and spiders. It has been used in a variety of areas, and especially for tissue-engineering scaffolds, due to its sound processability, mechanical properties, biodegradability, and biocompatibility. With respect to gelation, the SF gelation time is long in aqueous solutions, so a novel approach is needed to shorten this time. The solubility of regenerated SF is sound in formic acid (FA), which is a carboxylic acid of the simplest structure. In this study, SF was dissolved in formic acid, and the addition of salts then induced a rapid gelation that accompanied a solution-color change. Based on the gelation behaviors of the SF solution according to different SF and salt concentrations, the gelation mechanism was investigated.

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Effect of silk fibroin molecular weight on physical property of silk hydrogel

Cited by 66 publications

References 32 publications

Tailoring degradation rates of silk fibroin scaffolds for tissue engineering

Tailoring degradation rates of silk fibroin scaffolds for tissue engineering

A Review of Structure Construction of Silk Fibroin Biomaterials from Single Structures to Multi-Level Structures

Gelation Behaviors and Mechanism of Silk Fibroin According to the Addition of Nitrate Salts

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