Effect of silencing of the apoplastic invertase gene on photosynthesis in tomato

Chikov, V. I.; Akhtyamova, G. A.; Batasheva, Svetlana; Mikhailov, A. L.; Хамидуллина, Л. А.; Timofeeva, O. A.

doi:10.1134/s1021443715010045

Cited by 10 publications

(9 citation statements)

References 22 publications

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“…The answer is also obvious -in the building of cells with limited functionality (Figure 3). This leads to attenuation of the stomata closure mechanism in case of inconsistency between light and dark reactions of chloroplasts, as we have shown it in [10]. The increased flow of СО2 into the leaf probably even more enhances the carbohydrate orientation of photosynthesis.…”

Section: Introductionmentioning

confidence: 79%

Contradiction of Human Selection Activities to the Evolutionary Development of Ground Plants Will Lead to the Ecological Disaster

Ivanovich

2020

CIACR

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A contradiction has been established between human breeding activity and the evolutionary development of terrestrial plants, which lead to ecological disaster in the ocean. Experimental data are presented, and the conclusion is drawn that the elimination of these contradictions can not only maintain high productivity, restore soil fertility, but also get rid of a brewing ecological catastrophe in the oceans.

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Section: Introductionmentioning

confidence: 79%

Contradiction of Human Selection Activities to the Evolutionary Development of Ground Plants Will Lead to the Ecological Disaster

Ivanovich

2020

CIACR

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“…In plants grown in direct sunlight, photosynthesis and transpiration were measured simultaneously using a gasometric system Walz GFS-300 (Germany), which allowed calculation of the stomatal conductance for CO 2 diffusion. The results of the experiments showed [28] that, in the steady state, photosynthesis of transformants was only 9% higher than that of the wild type (Table 3). At the same time, transpiration and stomatal conductance did not differ significantly.…”

Section: The Regulatory Significance Of the Ratio Of Labeled Sucrose mentioning

confidence: 98%

“…On the contrary, experiments with tomato plants ( Table 2), in which the expression of apoplastic invertase in leaves was suppressed using RNA interference, showed [28] that invertase suppression increases photosynthesis (measured Table 1. The effect of HCl concentration (%) in a gasholder on the 14 C incorporation (% radioactivity of the water-ethanol soluble fraction) into some soluble products of 3-minute photosynthesis in cotton leaves [16].…”

Section: The Regulatory Significance Of the Ratio Of Labeled Sucrose mentioning

confidence: 99%

“…This became clear after gasometric studies. The turning point in understanding of the mechanism of photosynthesis regulation and the role of photorespiration in this regulation was the simultaneous measurement of water vapor and CO 2 gas exchange in tomato plants under changing light [28].…”

Section: The Regulatory Significance Of the Ratio Of Labeled Sucrose mentioning

confidence: 99%

“…As a result, stomatal conductance in the Table 2. Effect of suppression of the apoplastic invertase activity on leaf photosynthesis (kBq/(cm 2 min) in wild-type and transformed (Lin8RNAi) tomato plants of different ages [28]. wild type decreased (by 16%), and in transformed plants increased (by 24%).…”

Section: The Regulatory Significance Of the Ratio Of Labeled Sucrose mentioning

confidence: 99%

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Photorespiration and Its Role in the Regulation of Photosynthesis and Plant Productivity

Chikov¹,

Akhtyamova²

2019

AJPS

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The results of long-term studies of photorespiration are summarized and the unsuccessful attempts to increase productivity by suppressing this process are shown. It has been shown that photorespiration and glycolate metabolism are involved in the regulation of the relationship between light processes in chloroplasts and the dark reactions of carbon dioxide assimilation. The studies were conducted on plants in vivo and were associated with the activity of the apoplastic invertase enzyme, affecting assimilate transport. In violation of donor-acceptor relations between photosynthetic and plant-assimilating organs (removal of part of organs-consumers of assimilates or leaves, increase in nitrate nutrition), the kinetics of inclusion of 14 C in glycolate was changed. This is due to the strengthening of the role of the transketolase mechanism of its formation. The study of genetically transformed plants, in which either an additional apoplastic invertase gene was introduced, or the existing gene was blocked and did not act, showed a different change in the ratio of 14 C-labeled sucrose/hexose and the transpiration response to reduced light. In this connection, the concept of the mechanism of photorespiration interaction with apoplastic invertase and stomatal apparatus of the leaf is proposed when the ratio of light and dark reactions of photosynthesis or assimilate transport is changed. The essence of the concept is that when the ratio of light and dark processes is disturbed, the concentration of organic acids changes first in mesophilic cells (mainly by photorespiration), and then in the extracellular space. It changes the activity of apoplastic invertase, which hydrolyzes sucrose and prevents it from being exported from the leaf. Hydrolysis of sucrose increases the osmoticity of the aquatic environment of the apoplast, which increases with movement to the stomata. The changed osmoticity of the environment around the stomatal guard cells changes the resistance of CO 2 diffusion into the leaf. This normalizes the ratio of light and dark processes in the sheet. Therefore, when illumination decreases, nitrate nutrition increases or difficulties arise with the use of photosynthesis products in acceptor organs, the ratio of 14 C-labeled sucrose/hexose decreases, and the stomata close. With increasing illumination, reverse events occur.

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The influence of altered sink-source balance on the plant growth and yield of greenhouse tomato

Aslani

Gholami

Mobli

et al. 2020

Physiol Mol Biol Plants

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This experiment aimed to investigate the status of tomato plants in terms of sink or source-limitation of 2 cultivars of greenhouse tomato (Solanum lycopersicum L.), i.e., 'Grandella' and 'Isabella' under the greenhouse conditions of Iran and to improve the yield and plant growth by manipulating the sink-source balance. To this end, 4 treatments were applied: leaves were not pruned and fruits were pruned to one per truss (1F/3L), leaves were not pruned and fruits were pruned to two per truss (2F/3L), leaves were not pruned and fruits were pruned to three per truss (3F/3L) and no leaf and fruit pruning (control). The results showed that truss pruning reduced the sink demand and consequently, increased the amount of available assimilate for the growth of the remaining fruits or vegetative parts. The negative correlation between the leaf area index and the net assimilation rate and no significant difference in the net assimilation rate between different sink/source ratios showed that the excess leaf area index does not contribute in increasing the assimilate production and hence, total yield. Total fruit weight, harvest index, and the ratio of the ripe fruits to the total fruit led to the highest yield for control plants. No changes in chlorophyll, protein content and nitrate reductase activity were the evidence for the fact that sink/source ratio do not affect light-harvesting and light-utilizing components of photosynthesis. Since the individual weight of fruits increased with decreasing fruit number per trusses, the growth of individual fruits in both cultivars was source-limited and truss pruning can decrease this limitation. Future studies should be carried out to determine the best level of sink/source ratio that in addition to producing an acceptable amount of yield, meets the needs of consumers in the current stressful world by increasing the antioxidant and nutriceutical content of fruits.

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Effect of silencing of the apoplastic invertase gene on photosynthesis in tomato

Cited by 10 publications

References 22 publications

Contradiction of Human Selection Activities to the Evolutionary Development of Ground Plants Will Lead to the Ecological Disaster

Contradiction of Human Selection Activities to the Evolutionary Development of Ground Plants Will Lead to the Ecological Disaster

Photorespiration and Its Role in the Regulation of Photosynthesis and Plant Productivity

The influence of altered sink-source balance on the plant growth and yield of greenhouse tomato

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