Effect of silanization on chitosan porous scaffolds for peripheral nerve regeneration

Li, Guicai; Zhang, Luzhong; Wang, Caiping; Zhao, Xueying; Zhu, Cheng; Zheng, Yanhong; Wang, Yaling; Zhao, Ying‐Zheng; Yang, Yumin

doi:10.1016/j.carbpol.2013.09.064

Cited by 40 publications

(23 citation statements)

References 32 publications

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“…6d). Overlapped peaks of N-H stretching and O-H stretching vibrations visible around 3274 cm −1 indicate the presence of hydroxyl and amine groups [55]. Weaker band intensity is noted for CSAPiTi-pH 5 (Fig.…”

Section: Ftir-atr Analysismentioning

confidence: 86%

Relevant insight of surface characterization techniques to study covalent grafting of a biopolymer to titanium implant and its acidic resistance

D’Almeida

Amalric²,

Brunon³

et al. 2015

Applied Surface Science

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Section: Ftir-atr Analysismentioning

confidence: 86%

Relevant insight of surface characterization techniques to study covalent grafting of a biopolymer to titanium implant and its acidic resistance

D’Almeida

Amalric²,

Brunon³

et al. 2015

Applied Surface Science

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“…As collagen and chitosan are amongst the most abundant polymers in nature, each has been used in the design of drug delivery systems and for the formation of scaffolds in tissue engineering applications (Lee et al, 2008). Some studies have reported beneficial uses of collagen/chitosan scaffolds in the culturing of several cell types, including endothelial cells (Mori et al, 1998), fibroblasts (Mori et al, 1997), mesenchymal stem cells (Ragetly et al, 2010), nerve cells (Li et al, 2014) and chondrocytes (Lahiji et al, 2000). These scaffolds also have been used for tissue/organ engineering, including skin, bones, blood vessels and nerve conduits.…”

Section: Introductionmentioning

confidence: 99%

Effects of 3-dimensional culture conditions (collagen-chitosan nano-scaffolds) on maturation of dendritic cells and their capacity to interact with T-lymphocytes

Daneshmandi

Dibazar

Fateh

2015

Journal of Immunotoxicology

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In the body, there is a natural three-dimensional (3D) microenvironment in which immune cells, including dendritic cells (DC), play their functions. This study evaluated the impact of using collagen-chitosan 3D nano-scaffolds in comparisons to routine 2D culture plates on DC phenotype and functions. Bone marrow-derived DC were cultured on scaffolds and plates and then stimulated with lipopolysaccharide (LPS) or chitosan-based nanoparticles (NP) for 24 h. Thereafter, DC viability, expression of maturation markers and levels of cytokines secretion were evaluated. In another set of studies, the DC were co-cultured with allogenic T-lymphocytes in both the 2D and 3D systems and effects on DC-induction of T-lymphocyte proliferation and cytokine release were analyzed. The results indicated that CD40, CD86 and MHC II marker expression and interleukin (IL)-12, IL-6 and tumor necrosis factor (TNF)-secretion by DC were enhanced in 3D cultures in comparison to by cells maintained in the 2D states. The data also showed that DNA/chitosan NP activated DC more than LPS in the 3D system. T-Lymphocyte proliferation was induced to a greater extent by DNA/NP-treated DC when both cell types were maintained on the scaffolds. Interestingly, while DC induction of T-lymphocyte interferon (IFN)-and IL-4 release was enhanced in the 3D system (relative to controls), there was a suppression of transforming growth factor (TGF)-production; effects on IL-10 secretion were variable. The results here suggested that collagen-chitosan scaffolds could provide a proinflammatory and activator environment to perform studies to analyze effects of exogenous agents on the induction of DC maturation, NP uptake and/or cytokines release, as well as for the ability of these cells to potentially interact with other immune system cells in vitro.

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“…The porosity of the prepared hydrogels was evaluated by liquid displacement method as reported previously [31] and modified in this study. Briefly, the hydrogels were immersed into definite volume (V 1 ) of ethanol in a graduated cylinder for 10 min, the total volume of the ethanol-impregnated hydrogels along with ethanol was recorded as V 2 .…”

Section: Porositymentioning

confidence: 99%

“…Then the samples were put into a 24-well culture plate, and 500 μL L929 fibroblasts with concentration of 1×10 5 cells/mL in DMEM (containing 10% FCS, 100 U/ml Penicillin, and 100 μg/ml Streptomycin ) were seeded on the samples. The samples were incubated in a CO 2 incubator for 24 h, and then were fixed with 4% paraformaldehyde for at least 2 h. Thereafter, the cell morphology and distribution on various hydrogels were evaluated using TBO staining as described previously [31]. The quantity of fibroblasts on each hydrogel was statistically analyzed by randomly counting seven to nine images under optical microscopy (OM, Olympus).…”

Section: Cytotoxicity Testmentioning

confidence: 99%

Fabrication and characterization of polyacrylamide/silk fibroin hydrogels for peripheral nerve regeneration

Kong

Zhao

et al. 2015

Journal of Biomaterials Science, Polymer Edition

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Various hydrogels have been used for repairing peripheral nerve injury; however, the silk fibroin (SF)-based hydrogels in peripheral nerve regeneration are still rarely reported. In this study, the SF/pAM hydrogels with different SF concentrations and ethanol treatment time were developed by solution blending and in situ radical polymerization. The physiochemical properties of composite hydrogels were measured, the cytotoxicity of hydrogels was evaluated by L929 fibroblasts, and the effect on peripheral nerve regeneration was evaluated via Schwann cells culture in vitro. The results showed that the physiochemical properties of SF/pAM hydrogels could be changed by varying SF concentration and ethanol treatment time, and the mechanical property was enhanced with increasing SF concentration, while the presence of SF in pAM hydrogels and ethanol treatment does not affect hydrogels structure in per se. All the composite hydrogels displayed no obvious cytotoxicity, while the SF/pAM composite hydrogels with 10% SF and 60-min ethanol treatment could obviously accelerate the attachment and proliferation of Schwann cells. Therefore, the SF/pAM composite hydrogels possessed the beneficial properties required for in situ cell scaffolding and may have potential application in peripheral nerve regeneration.

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Effect of silanization on chitosan porous scaffolds for peripheral nerve regeneration

Cited by 40 publications

References 32 publications

Relevant insight of surface characterization techniques to study covalent grafting of a biopolymer to titanium implant and its acidic resistance

Relevant insight of surface characterization techniques to study covalent grafting of a biopolymer to titanium implant and its acidic resistance

Effects of 3-dimensional culture conditions (collagen-chitosan nano-scaffolds) on maturation of dendritic cells and their capacity to interact with T-lymphocytes

Fabrication and characterization of polyacrylamide/silk fibroin hydrogels for peripheral nerve regeneration

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