Effect of sialoadenectomy on the carriage of <i>Candida albicans</i> in the mouths of rats

Jorge, Antônio Olavo Cardoso; Totti, Marilda Aparecida Gonçalves; Almeida, OP; Scully, Crispian

doi:10.1111/j.1600-0714.1993.tb01045.x

Cited by 27 publications

(27 citation statements)

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“…The transformation from the saprophytic to the parasitic form is related to several factors including not only the virulence of these yeasts but also host-related variables. Among the host-related factors, the salivary anti-Candida immunoglobulins and cellular defenses play an essential role for controlling the infection 13,18 . When these defenses are compromised, such as in cases of xerostomia, candidosis can occur.…”

Section: Discussionmentioning

confidence: 99%

Oral candidosis by Candida albicans in normal and xerostomic mice

et al. 2004

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The aim of this study was to analyze the effect of sialoadenectomy on the development of oral candidosis after one or four inoculations of Candida albicans. Initially, a suspension containing 10(8) cells/ml of C. albicans ATCC 36801 was prepared. Seventy-eight sialoadenectomized mice and a similar amount of mice with normal salivary flow received a single inoculation of C. albicans suspension. Another group with a similar number of mice received 4 inoculations. The control group consisted of 6 sialoadenectomized mice and 6 mice with normal salivary flow that were not inoculated with C. albicans. Candidosis development was studied histologically in the tongue of the animals 1, 2, 3, 5, and 8 days after inoculation and at 15-day intervals up to 165 days. According to the results obtained, it could be concluded that sialoadenectomy and a higher frequency of yeast inoculation influenced the presence and extension of candidosis lesions.

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Section: Discussionmentioning

confidence: 99%

Oral candidosis by Candida albicans in normal and xerostomic mice

et al. 2004

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“…As in humans, xerostomia in rats facilitates the establishment and persistence of C. albicans infection in the mouth; therefore, it constitutes a suitable animal model for the study of oral candidiasis (25). Sialoadenectomy in rats causes intense xerostomia, but the minor salivary glands, the main producers of mucin, an important barrier for mucosal permeability and a major source of immunoglobulin A, were preserved.…”

Section: Methodsmentioning

confidence: 99%

“…Dilutions up to 10 Ϫ5 (0.1 ml) were cultured in duplicate in Sabouraud's dextrose agar at 37°C for 48 h. Candida colonies were counted in plates exhibiting between 30 and 300 colonies. Plates with less than 30 colonies in the 10 Ϫ2 dilution were considered to have 10 1 cells (25). Clinical lesions.…”

Section: Methodsmentioning

confidence: 99%

“…Statistical analysis of the quantitation of C. albicans cells in oral tissue was performed by one-way analysis of variance, followed by Bonferroni's t test. The percentage of areas of clinical lesions was analyzed by Student's t test (25). The Wilcoxon signed-rank sum test for paired comparisons and the Kruskal-Wallis test for multiple comparisons were used to determine the degree of colonization of the epithelium by fungal hyphae (33).…”

Section: Methodsmentioning

confidence: 99%

“…Establishment of C. albicans infection was evaluated by swabbing the inoculated oral cavity with a sterile cotton applicator, followed by plating on YEPD agar (25,31). Samples were collected 2 days after inoculation and at the end of experiment.…”

Section: Methodsmentioning

confidence: 99%

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Effects of Psychological Stress and Alprazolam on Development of Oral Candidiasis in Rats

Núñez

Balboa

Riveiro

et al. 2002

Clin Vaccine Immunol

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Psychological stress has been found to suppress cell-mediated immune responses that are important in limiting the proliferation of Candida albicans. Since anxiolytic drugs can restore cellular immunity in rodents exposed to stress conditions, we designed experiments conducted to evaluate the effects of alprazolam (1 mg/kg of body weight/day), a central benzodiazepine anxiolytic agonist, on the development of oral candidiasis in Sprague-Dawley rats exposed to a chronic auditory stressor. Animals were submitted to surgical hyposalivation in order to facilitate the establishment and persistence of C. albicans infection. Application of stress and treatment with drugs (placebo or alprazolam) were initiated 7 days before C. albicans inoculation and lasted until the end of the experiments (day 15 postinoculation). Establishment of C. albicans infection was evaluated by swabbing the inoculated oral cavity with a sterile cotton applicator on days 2 and 15 after inoculation, followed by plating on YEPD (yeast extract-peptone-dextrose) agar. Tissue injury was determined by the quantification of the number and type (normal or abnormal) of papillae on the dorsal tongue per microscopic field. A semiquantitative scale was devised to assess the degree of colonization of the epithelium by fungal hyphae. Our results show that stress exacerbates C. albicans infection of the tongues of rats. Significant increases in Candida counts, the percentage of the tongue's surface covered with clinical lesions, the percentage of abnormal papillae, and the colonization of the epithelium by fungal hyphae were found in stressed rats compared to those found in the unstressed rats. Treatment with alprazolam significantly reversed these adverse effects of stress, showing that, besides the psychopharmacological properties of this anxiolytic drug against stress, it has consequences for Candida infection.

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