Effect of sialoadenectomy and salivary gland extracts on gastrointestinal mucosal growth and gastrin levels in the rat.

Abstract

SUMMARY1. We have examined gastrointestinal mucosal growth 30 days after surgical removal of the submandibular-sublingual salivary glands and ligation of the parotid ducts of rats. The rate of [3H]thymidine uptake in vitro as an estimation of DNA synthesis and the content of DNA and RNA were examined in the oxyntic, duodenal and proximal colonic mucosa.2. DNA synthesis, DNA and RNA content of oxyntic mucosa were reduced in sialoadenectomized rats when compared to sham-sialoadenectomized control animals. There was no change in the degree of [3H]thymidine incorporation or DNA content of the duodenal or colonic mucosa.3. Intraperitoneal injection ofan aqueous extract ofthe submandibular-sublingual salivary glands of rats (4-0 mg tissue protein in 0-1 M-sodium phosphate buffer administered twice a day for 15 days) increased the rate of DNA synthesis and the total mucosal DNA and RNA content in the oxyntic mucosa. Injections of extracts of spleen or muscle did not produce consistent results.4. Administration of epidermal growth factor (10 jug/kg) or pentagastrin (250 psg/kg) resulted in an increase of the level of DNA synthesis observed in the oxyntic mucosa of sialoadenectomized rats. 5. Plasma and antral tissue levels of the trophic hormone, gastrin, were not significantly decreased in sialoadenectomized rats treated with 041 M-sodium phosphate-buffered saline. However, treatment with the salivary tissue extract did result in significant reductions in both plasma and tissue levels of gastrin.6. We conclude that elimination of the major salivary glands in the rat results in a decrease in [3H]thymidine uptake and DNA content of the gastric oxyntic mucosa. These effects are not mediated via a reduction in endogenous levels of the trophic hormone, gastrin. Administration of an aqueous extract of salivary tissue exerted a small but significant trophic influence on the oxyntic mucosa of the rat.

Effect of nitric oxide on integrity, blood flow and cyclic GMP levels in the rat gastric mucosa: modulation by sialoadenectomy

“…Sialoadenectomy was performed under pentobarbitone anaesthesia (60mg kg-', i.p.) by removal of the submandibularsublingual gland complexes after the ducts were ligated as previously described (Skinner & Tepperman, 1981;Skinner et al, 1984). SALX and sham-operated animals were administered an antibiotic (Pen-Di-Strep 0.1 ml, i.m.)…”

Effect of a submaxillary gland extract on Ehrlich tumor growth in mice

“…Cell viability was always at least 90%, and the cell concentration for inoculation was 4.5 x 10 7 viable cells/ml. SGE was prepared by the method of Skinner et al (6). One hundred pairs of submaxillary glands were harvested from adult male mice and homogenized with a manual tissue homogenizer and 0.3 ml of 0.2% (w/v) soybean trypsin inhibitor (Sigma Chemical Co., St. Louis, MO, USA) and 7.7 ml PBS/g gland were added at 4 o C. The homogenate was centrifuged at 20,000 g for 30 min at 4 o C and the supernatant was stored at -30 o C until use.…”

Localization of transforming growth factor alpha and its receptor in gastric mucosal cells. Implications for a regulatory role in acid secretion and mucosal renewal.

“…Removal of salivary glands (the major intraluminal source of EGF) in rats results in atrophy of gastric mucosa (17) and a delay in healing ofgastric and duodenal ulcerations that can be reversed by treating the animals with either oral or parenteral EGF (18). These studies imply an important physiological role for EGF in gastric mucosal growth.…”