Effect of short-term treatment with potassium permanganate on stress markers and blood biochemistry in goldfishCarassius auratus

Abstract

Potassium permanganate (PM) is a therapeutic agent in aquaculture with strong oxidant properties. In this study, the effects of potassium permanganate treatment (PMT) on stress response and blood biochemistry were investigated in goldfish Carassius auratus. Two triplicate groups of fish were exposed to either 10 mg L À1 PM or clean water, over 30 min. Blood samples were collected at 0 and 0.5 h after exposure as well as 3 and 24 h. PMT caused significant increase in serum cortisol and glucose levels, 0.5 h post exposure, which stayed elevated until 24 h. Sodium decreased after exposure and did not return to initial levels. Chloride decreased at 0.5 and 3 h, but returned to initial levels at 24 h. Calcium decreased at 0.5 h and returned to initial at 3 h, but significantly increased at 24 h. Total protein, albumin and globulin levels increased at 3 h and stayed elevated until 24 h. Albumin:globulin ratio (A:G) levels decreased at 24 h post exposure. Results indicated that short-term PMT caused stress response, hydromineral imbalance and possibly change in blood protein profile in goldfish, which lasted until 24 h post treatment.

Physiological, ionoregulatory, metabolic and immune responses of Persian sturgeon,Acipenser persicus(Borodin, 1897) to stress

“…However, serum calcium increased after stress probably due to the stimulating effect of cortisol on calcium uptake carriers in the gills, as suggested by Flik and Perry (). Similarly, Hoseini and Tarkhani (,b) found a significant increase in serum calcium concentration in Carassius auratus after cortisol elevation due to NaCl, formalin or potassium permanganate exposure.…”

Plasma proteins, hepatic enzymes, thyroid hormones and liver histopathology of Cyprinus carpio (Linnaeus, 1758) exposed to an oxadiazin pesticide, indoxacarb

“…Fish plasma protein is an important compound with several roles such as transporting the other compounds, immune function and control of the osmotic pressure. Also, proteins are important biochemical indicators, which are used to recognize the fish health, physiology and metabolism under pesticides intoxication (Clark & Holling, ; Corbel, ; Hoseini & Tarkhani, ; Silva, Cortez‐Moreira, Bastos, Bastos & Cortez, ). Total protein, albumin and globulin tests are used to monitor each disorder in the immune system and dysfunction of liver and kidney (Banaee, Mirvagefei, Rafei & Amiri, ; John, ).…”

“…However, plasma globulin level increased at 3 ppm treatment of indoxacarb after 7 days. The levels of plasma proteins might change as a result of haemoconcentration, haemodilution, stress response and change in protein synthesis of the liver (Hoseini & Tarkhani, ). As the levels of albumin had no significant changes during the indoxacarb exposure in this study, it is hypothesized that no haemodilution happened in the indoxacarb‐treated fish.…”

“…The plasma ALT and AST activity indicated the liver impairment occurred in all indoxacarb‐exposed fish, and the damage was probably depended on the dose of indoxacarb. Increased activity of AST and ALT observed in different fish species which exposed to various toxicants (Agrahari, Pandey & Gopal, ; Banaee et al., ; Hoseini, Hosseini & Soudagar, ; Hoseini & Tarkhani, ). In the present study, plasma ALT and AST showed elevation only at the first sampling time, despite the hepatic damages at the third sampling time.…”

“…Liver can produce another enzyme named ALP via the cells lining the small bile; whereas, this enzyme is found in different body tissues in addition to liver (Agrahari & Gopal, ). Toxicants may increase (Hoseini, Hedayati, Mirghaed & Ghelichpour, ; Hoseini & Tarkhani, ; Karan, Vitorović, Tutundžić & Poleksić, ) and decrease (Öner, Atli & Canli, ) plasma ALP activity. It was suggested that plasma ALP elevation could be related to tissue damage, especially, when the hepatobiliary problems occur; however, its demotion might be due to an impairment of the membrane transport system (Bernet, Schmidt, Wahli & Burkhardt‐Holm, ; Haschek et al., ).…”