Effect of short-term exposure to low concentration of tebuconazole: morphological, histometric and functional modifications in <i>Danio rerio</i> liver

Macirella, Rachele; Curcio, Vittoria; Ahmed, Abdalmoiz I. M.; Pellegrino, Daniela; Brunelli, Elvira

doi:10.1080/24750263.2022.2043469

Cited by 7 publications

(3 citation statements)

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“…When a fish is consumed, the pesticides accumulated in its edible tissue can be transferred to humans. Liver tissue is the detoxification center and stores toxicants in many studied fish species (Braunbeck et al, 1998;Macirella et al, 2022). In this study, however, some pesticides, e.g., pyrimethanil, were found in higher concentrations in the fillet tissue of L.…”

Section: Discussioncontrasting

confidence: 52%

Pesticide concentration in three selected fish species and human health risk in the Lake Tana sub-basin, Ethiopia

Zelalem,

Anteneh,

Mingist

et al. 2023

Environ Monit Assess

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y. The VOR is the version of the article after copy-editing and typesetting, and connected to open research data, open protocols, and open code where available. Any supplementary information can be found on the journal website, connected to the VOR.For research integrity purposes it is best practice to cite the published Version of Record (VOR), where available (for example, see ICMJE's guidelines on overlapping publications). Where users do not have access to the VOR, any citation must clearly indicate that the reference is to an Accepted Manuscript (AM) version.

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Section: Discussioncontrasting

confidence: 52%

Pesticide concentration in three selected fish species and human health risk in the Lake Tana sub-basin, Ethiopia

Zelalem,

Anteneh,

Mingist

et al. 2023

Environ Monit Assess

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“…The morphology of the Danio rerio liver is similar to that of other freshwater Teleosts [53,54], and only a brief description will be given in the present study. The parenchymal mass, even and compact, was crossed by a network of sinusoids surrounded by cords of hepatocytes (Figure 1a,b).…”

Section: Control Groupmentioning

confidence: 99%

Morphological and Functional Alterations in Zebrafish (Danio rerio) Liver after Exposure to Two Ecologically Relevant Concentrations of Lead

Macirella

Curcio

Ahmed

et al. 2023

Fishes

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Lead (Pb) is a non-essential, highly toxic, and persistent element widely recognized as one of the most concerning pollutants. It is listed on the Priority List of Hazardous Substances. Widespread environmental contamination from Pb is a serious issue for human health and wildlife. In fish, Pb mainly accumulates in the liver, which is a key component for metal detoxification and excretion processes. In this study, we investigated, for the first time, the morphological and functional injuries induced in zebrafish (Danio rerio) liver by two very low and environmentally relevant concentrations of Pb (2.5 and 5 μg/L) after 48, 96, and 192 h of exposure. We observed significant histological alterations in all the exposed samples, and it was demonstrated that the extent of injuries increased with dose and exposure time. The most common modifications observed were congestion of blood vessels and sinusoids, cytoplasmic vacuolizations, parenchyma dyschromia, and macrophage proliferation. Pb administration also resulted in a significant increase in lipid content and the upregulation of key genes that are involved in metal detoxification (mtf1) and the defensive response against oxidative stress (sod1 and cat). We show that even very low doses of Pb can disrupt liver morphology and function.

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“…Both in vitro and in vivo studies demonstrated that in fish, Pb exposure stimulated reactive oxygen species (ROS) production resulting in oxidative damage and increasing antioxidant responses [ 17 , 50 ]. An important group of enzymes plays the role of scavenger compounds against free radicals, including superoxide dismutase (SOD), which is considered the primary intracellular antioxidant defense against free radicals [ 51 , 52 ]. To the best of our knowledge, the study presented here is the first report focusing on the morphological, morphometric, and functional alterations induced by non-lethal Pb doses in zebrafish gills; moreover, given the high translational value of the experimental model, the present results would also contribute to the discussion regarding Pb toxicity in humans.…”

Section: Introductionmentioning

confidence: 99%

Morphological and Functional Alterations Induced by Two Ecologically Relevant Concentrations of Lead on Danio rerio Gills

Curcio

Macirella

Sesti

et al. 2022

IJMS

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Lead (Pb), due to its high toxicity and bioaccumulation tendency, is one of the top three pollutants of concern for both humans and wildlife and occupies second place in the Priority List of Hazardous Substances. In freshwater fish, Pb is mainly absorbed through the gills, where the greatest accumulation occurs. Despite the crucial role of gills in several physiological functions such as gas exchange, water balance, and osmoregulation, no studies evaluated the effects of environmentally relevant concentrations of Pb on this organ, and existing literature only refers to high levels of exposure. Herein we investigated for the first time the molecular and morphological effects induced by two low and environmentally relevant concentrations of Pb (2.5 and 5 μg/L) on the gills of Danio rerio, a model species with a high translational value for human toxicity. It was demonstrated that Pb administration at even low doses induces osmoregulatory dysfunctions by affecting Na+/K+-ATPase and AQP3 expression. It was also shown that Pb upregulates MTs as a protective response to prevent cell damage. Modulation of SOD confirms that the production of reactive oxygen species is an important toxicity mechanism of Pb. Histological and morphometric analysis revealed conspicuous pathological changes, both dose- and time-dependent.

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Effect of short-term exposure to low concentration of tebuconazole: morphological, histometric and functional modifications in Danio rerio liver

Cited by 7 publications

References 58 publications

Pesticide concentration in three selected fish species and human health risk in the Lake Tana sub-basin, Ethiopia

Pesticide concentration in three selected fish species and human health risk in the Lake Tana sub-basin, Ethiopia

Morphological and Functional Alterations in Zebrafish (Danio rerio) Liver after Exposure to Two Ecologically Relevant Concentrations of Lead

Morphological and Functional Alterations Induced by Two Ecologically Relevant Concentrations of Lead on Danio rerio Gills

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