Effect of Short-term and Long-term Preservation on Motion Characteristics of Garole Ram Spermatozoa: A Prolific Microsheep Breed of India

Joshi, Anil; Bag, Sadhan; Naqvi, S. M. K.; Sharma, R.C.; Rawat, P. S.; Mittal, J. P.

doi:10.5713/ajas.2001.1527

Cited by 11 publications

(11 citation statements)

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“…However, severe reduction in the sperm motility was observed for the EYMG followed by EYC and EYCF compared with the TCEYF extender. The much lower sperm motility observed in case of EYMG extended epididymal spermatozoa in the present study might be due to the variation in the source of spermatozoa (cauda epididymidis), breeds of ram and processing procedure (double centrifugation) before extension and preservation than the report of Joshi et al. (2001).…”

Section: Discussioncontrasting

confidence: 63%

“…Except the TCEYF, other three extenders showed much lower sperm motility than the previous works done with EYC extender for preservation of epididymal spermatozoa (Lone et al. 2011) and EYC (Islam and Khan 2010), EYMG (Joshi et al. 2001) and Cornell University Extender (George et al.…”

Section: Discussionmentioning

confidence: 72%

“…EYMG was worst of all ‐ as it could yield poor motility after centrifugation (Table 1). Joshi et al. (2001) recorded 90.1%, 85.5% and 73.2% sperm motility at 0 h, 24 h and 48 h of storage of ejaculated Garole ram semen extended with EYMG at refrigeration temperature.…”

Section: Discussionmentioning

confidence: 88%

“…Research works using various extenders for short‐term preservation of ejaculated spermatozoa from rams (Kakadiya and Kavani 1995; Bozkurt et al. 1999; Joshi et al. 2001; Paulenz et al.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Different Egg Yolk‐Based Extenders on the Quality of Ovine Cauda Epididymal Spermatozoa during Storage at 4°C

Lone¹,

Islam²,

Sofi³

2011

Reprod Domestic Animals

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Cauda epididymal spermatozoa were obtained from testicles collected from abattoir(s). The pooled sperm samples were divided into four aliquots. Each aliquot was washed separately with the buffer of respective extender and finally extended with the four extenders viz. egg yolk-citrate (EYC), egg yolk-citrate-fructose (EYCF), Tris-citric acid-egg yolk-fructose (TCEYF) and egg yolk-Mcillvaine glucose (EYMG) and preserved at 4°C. The per cent sperm motility for EYC, EYCF, TCEYF and EYMG at 0 h was 50.83%, 56.67%, 75.00% and 31.67%, respectively, and at 72 h was 24.17% (EYC), 30.83% (EYCF), 51.67% (TCEYF) and 7.50% (EYMG). The corresponding figures for live sperm count at 0 h was 83.17%, 86.33%, 90.42% and 81.75% and at 72 h was 64.75%, 73.92%, 76.00% and 57.67%. The corresponding figures for mean per cent intact acrosome at 0 h was 95.33%, 95.50%, 90.92% and 97.25% and at 72 h was 86.17%, 83.92%, 77.58% and 86.33%. The sperm motility was significantly (p < 0.05) higher for TCEYF at different h of preservation from 0 h through 72 h. The sperm motility, live sperm count and per cent intact acrosome declined significantly (p < 0.05) with the advancement of storage time in all the four extenders. Our study concluded that TCEYF was best out of the extenders studied for preservation of cauda epididymal spermatozoa after double centrifugation and extension at 4°C up to 72 h of preservation. However, EYCF also has better potential for the preservation of cauda epididymal spermatozoa as viability was in close proximity and acrosomal integrity was higher compared with TCEYF extender.

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Section: Discussioncontrasting

confidence: 63%

Section: Discussionmentioning

confidence: 72%

Section: Discussionmentioning

confidence: 88%

“…Research works using various extenders for short‐term preservation of ejaculated spermatozoa from rams (Kakadiya and Kavani 1995; Bozkurt et al. 1999; Joshi et al. 2001; Paulenz et al.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Different Egg Yolk‐Based Extenders on the Quality of Ovine Cauda Epididymal Spermatozoa during Storage at 4°C

Lone¹,

Islam²,

Sofi³

2011

Reprod Domestic Animals

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show abstract

“…(Mittal and Ghosh, 1979;Galil and Galil, 1982;Ibrahim, 1997). The CASA technique provides precise and validated objective sperm motion characteristics (Holt and Palomo, 1996) and has been extensively applied for ram spermatozoa (Edward et al, 1995;Briggs et al, 1996;Joshi et al, 2001;2003;2005;Bag et al, 2002;2004;Kumar et al, 2007;2010); however, care must be taken in preparation of the sample and choosing settings of the instrument (Davis and Siemers, 1995). Davis and Katz (1992; reported that results of CASA are inaccurate if the sperm count for analysis, is below 20 Â 10 6 sperm/ml or above 50 Â 10 6 sperm/ml.…”

Section: Discussionmentioning

confidence: 99%

Sperm motion characteristics of FecBBB and FecBB+ Garole x Malpura rams during the non-breeding season under hot semi-arid environment

Kumar¹,

Naqvi²,

Kumar

2012

Livestock Science

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Sperm motion characteristics of Garole×Malpura sheep evolved in a semi-arid tropical environment through introgression of FecB gene

Kumar

Joshi

Naqvi

et al. 2007

Animal Reproduction Science

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Effect of Short-term and Long-term Preservation on Motion Characteristics of Garole Ram Spermatozoa: A Prolific Microsheep Breed of India

Cited by 11 publications

References 0 publications

Effect of Different Egg Yolk‐Based Extenders on the Quality of Ovine Cauda Epididymal Spermatozoa during Storage at 4°C

Effect of Different Egg Yolk‐Based Extenders on the Quality of Ovine Cauda Epididymal Spermatozoa during Storage at 4°C

Sperm motion characteristics of FecBBB and FecBB+ Garole x Malpura rams during the non-breeding season under hot semi-arid environment

Sperm motion characteristics of Garole×Malpura sheep evolved in a semi-arid tropical environment through introgression of FecB gene

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