Effect of Short Period Vasectomy on FSH, LH, Inhibin and Testosterone Secretions, and Sperm Motility in Adult Male Rats

Ren, Longquan; Wang, Qiang; Kishimoto, Miori; Watanabe, Gen; Jaroenporn, Sukanya; Taya, Kazuyoshi

doi:10.1538/expanim.60.47

Cited by 13 publications

(13 citation statements)

References 51 publications

(54 reference statements)

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“…reported that vasectomy did not affect testosterone levels. However, other studies have reported a significant reduction in testosterone during the early stages after vasectomy 43. Our data demonstrate that testosterone decreases significantly after vasectomy and gradually returns to baseline levels.…”

Section: Discussionsupporting

confidence: 55%

Expression and function of HSP110 family in mouse testis after vasectomy

Rong

Shi

et al. 2017

Asian J Androl

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HSP110 functions to protect cells, tissues, and organs from noxious conditions. Vasectomy induces apoptosis in the testis; however, little is known about the reason leading to this outcome. The aim of the present study was to evaluate the expression and function of HSP110 in mouse testis after vasectomy. Following bilateral vasectomy, we used fluorescent Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) to detect apoptosis, Western blotting and immunohistochemistry to examine HSP110 expression and localization. Serum antisperm antibody (AsAb) and testosterone were measured by Enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay, respectively. Expression of endoplasmic reticulum stress (ERS) sensors and downstream signaling components was measured by Reverse Transcription-Polymerase Chain Reaction (RT-PCR), and the phosphorylation of eIF2α and JNK was detected by Western blotting. Vasectomy induced morphologic changes, increased apoptosis in the testis, increased serum AsAb, and decreased testosterone levels. After vasectomy, ORP150 mRNA level was increased first and then decreased, Bcl-2 was decreased, and the expression of HSPA4l, GRP78, GADD153, PERK, ATF6, IRE-1, XBP-1s, Bax, Bak, and caspases and the phosphorylation of eIF2α and JNK were increased. We present that an ER stress-mediated pathway is activated and involved in apoptosis in the testis after vasectomy. HSPA4l and ORP150 may play important roles in maintaining the normal structure and function of testis.

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Section: Discussionsupporting

confidence: 55%

Expression and function of HSP110 family in mouse testis after vasectomy

Rong

Shi

et al. 2017

Asian J Androl

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“…A significant increase in epididymal weight was seen in vasectomized mice in comparison to the control group. Ren et al (2011a) reported a significant increase of epididymal weight in vasectomized group until 30 days post operation compared with control group as well. However, the weights of epididymis decreased from 30 days post operation compared with control group.…”

Section: Discussionmentioning

confidence: 84%

“…This effect of vasectomy has been reported in other studies as well. For example, Ren et al (2011a), who investigated the effect of short period vasectomy on sperm motility in adult male rats, found that vasectomy can harm spermatogenesis, reducing sperm motility starting from day 60 after vasectomy. Silber et al (1995), have suggested that in cases of chronically obstructed epididymis, sperm suffer changes related to old age with a significant decrease in motility towards the distal part.…”

Section: Discussionmentioning

confidence: 99%

“…These cells are penetrated into the epididymis epithelium from the circulation system. In a survey on the effects of chronic vasectomy in laboratory animals (hamster, rat and monkey) a rapid formation of sperm granulomas in nearly all cases, either at the site of ligation on the vas deferens or in the tail of epididymis was reported (Ren et al, 2011b). Alexander & Anderson (1979), found that the presence of immunocompetent cells in different parts of the epididymis is important because an individual is capable of mounting an immune response to his own spermatozoa.…”

Section: Discussionmentioning

confidence: 99%

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The Effects of Vasectomy on Epididymal Morphology and Sperm Parameters in Adult Male Balb/c mice

et al. 2013

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SUMMARY:Vasectomy is a common contraceptive procedure in men. The present study is aimed to explore the impact of vasectomy on epididymal morphology and sperm parameters in adult male Balb/c mice. Twenty adult (age: 8 weeks) male Balb/c mice, weighting 20-30 g were used in the experiments. They were divided into 2 groups (vasectomy and sham). The operations were performed under sodium pentobarbital (40 mg/kg body weight, IP) anesthesia via a lower mid-abdominal incision. The left epididymis caput was fixed for histological studies and the right one was used for sperm count and motility. Progressive fast and slow sperm motility were significantly decreased in the vasectomized compared to the sham operation group (P<0.05) and the number of immotile sperm in the vasectomized group was increased in comparison to control group. Sperm granuloma was seen in 60% of epididymis after vasectomy. Also, Histological study showed an increase in tubular lumen diameter, interstitial space and infiltration of immune cells in interstitial tissue in vasectomized group. Vasectomy increases histopathological changes in epididymis and decreases the motility of sperm developing a reduction in fertility rates.

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“…Subsequently, loss of Bbx caused azoospermia and infertility. Aberrant multinucleated giant cells were previously observed in rats with vasectomy-induced spermatogenic impairments [31]. In addition, genetically modified mice, such as Crem - [29, 30], Trf2 - [32], and Fdnc3a -deficient mice [33], display spermatogenic defects with aberrant multinucleated giant cells in the seminiferous tubules.…”

Section: Discussionmentioning

confidence: 99%

PiggyBac Transposon-Mediated Mutagenesis in Rats Reveals a Crucial Role of Bbx in Growth and Male Fertility

Wang¹,

Tang

Chang

et al. 2016

Biology of Reproduction

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Bobby sox homolog (Bbx) is an evolutionally conserved gene, but its biological function remains elusive. Here, we characterized defects of Bbx mutant rats that were created by PiggyBac-mediated insertional mutagenesis. Smaller body size and male infertility were the two major phenotypes of homozygous Bbx mutants. Bbx expression profile analysis showed that Bbx was more highly expressed in the testis and pituitary gland than in other organs. Histology and hormonal gene expression analysis of control and Bbx-null pituitary glands showed that loss of Bbx appeared to be dispensable for pituitary histogenesis and the expression of major hormones. BBX was localized in the nuclei of postmeiotic spermatids and Sertoli cells in wild-type testes, but absent in mutant testes. An increased presence of aberrant multinuclear giant cells and apoptotic cells was observed in mutant seminiferous tubules. TUNEL-positive cells costained with CREM (round spermatid marker), but not PLZF (spermatogonia marker), gammaH2Ax (meiotic spermatocyte marker), or GATA4 (Sertoli cell marker). Finally, there were drastically reduced numbers and motility of epididymal sperm from Bbx-null rats. These results suggest that loss of BBX induces apoptosis of postmeiotic spermatids and results in spermiogenesis defects and infertility.

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Effect of Short Period Vasectomy on FSH, LH, Inhibin and Testosterone Secretions, and Sperm Motility in Adult Male Rats

Cited by 13 publications

References 51 publications

Expression and function of HSP110 family in mouse testis after vasectomy

Expression and function of HSP110 family in mouse testis after vasectomy

The Effects of Vasectomy on Epididymal Morphology and Sperm Parameters in Adult Male Balb/c mice

PiggyBac Transposon-Mediated Mutagenesis in Rats Reveals a Crucial Role of Bbx in Growth and Male Fertility

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