Effect of short-chain fatty acid on sodium absorption in isolated human colon perfused through the vascular bed

Abstract

A method of perfusing the isolated human colon in vitro was developed to study the effect of the short-chain fatty acid n-butyrate on sodium absorption under controlled conditions. The isolated colon was viable in vitro provided that ischemia to the colon prior to perfusion was less than 40 min. Viability was judged on glucose utilization, mucosal potential difference, an sodium absorption. Sodium absorption from the lumen was observed either with or without 20 mM n-butyrate. In a control group sodium absorption (nmol/min/cm2 /+- SEM) was 320 /+- 10 (four perfusions, nine observation intervals) and potassium secretion 26 /+- 3 (four perfusions, nine observation intervals). With 20 mM n-butyrate sodium absorption was 1960 /+- 480 (four perfusions, ten observation intervals) (P less than 0.0025). Potassium secretion was 72 /+- 2 (four perfusions, ten observation intervals) and (P less than 0.025). Butyrate absorption was 254 /+- 60 (four perfusions, ten observation intervals) and correlated linearly with the unidirectional flux (Jm leads to S) of sodium (linear coefficient of 0.714, P = less than 0.001). These results suggest that the presence of bacterial short-chain fatty acids may determine the efficiency of sodium absorption in the colon and also indicate that an absence of short-chain fatty acids in the colon could be one factor leading to diminished sodium absorption in the colon of man.

Stimulation of Butyrate Production in the Large Intestine of Weaning Piglets by Dietary Fructooligosaccharides and Its Influence on the Histological Variables of the Large Intestinal Mucosa

“…Increases in the morphometric variables were princi pally correlated with the luminal n-butyrate and n-val erate concentrations (Table 3). The role of n-butyrate has been repeatedly emphasized in the physiology of the large intestine; it stimulates epithelial cell proliferation (13,14), mineral and water absorption (15,16), and mucus secretion (12). Furthermore, it has an anti-tum origenic effect in the large intestine (22,44,45).…”

“…n -Butyrate is the major energy source for the epithelial cells of the large intestine (10,11) and stimulates mucus release (12) and epithelial cell proliferation (13,14) as well as mineral and water absorption from the lumen (15,16). Therefore, the effect of FOS on hindgut physiology should be realized through an increase in luminal n-butyrate concentration.…”

Incidence of the circadian rhythm of the excretion pattern on acetate absorption and metabolism in the rabbit hind-gut

“…A polythene catheter (PE 90) was inserted into one of the small caecocolonic veins draining each loop, and care was taken to ensure that the blood circulation was not damaged. A carotid artery was exposed and cannulated (PE 190 (Dobson and Phillipson, 1956;Bensadoun et al, 1962;Sellers, 1965), dogs (Kvietys and Granger, 1981), and rats (Demigné and Rémésy, 1985) (Vernay, 1987b) or butyrate (Vernay, 1987c propionate (Vernay, 1987b) or butyrate (Vernay, 1987c (Clauss and Hornicke, 1979a,b; and corticosterone (Bonnafous and Raynaud, 1968;Kawakami et aL, 1972) are doubled, it appears that the absorptive processes Vernay, 1987a,d) (Roediger, 1980;Roediger and Moore, 1981;Cummings, 1984), rat (Roediger, 1982) (Aikawa et al, 1972;Felig, 1973). The presence of an acetyl-CoA synthetase mainly located in the cytosol fraction (Woodnutt and Parker, 1978) indicates that in rabbit liver, as in rat liver (Hanson and Ballard, 1967;Knowles et al, 1974;Scholte and Groot, 1975 (Beauville et al, 1974;McMillan et al, 1975;Bonnafous and Raynaud, 1978;Vernay, 1987a (Drury and Wick, 1956;Vezinhet and Nougu b .., 1977;Jones andParker, 1977, 1981 ).…”

Physiological Functions of Partially Hydrolyzed Guar Gum

“…The SCFAs are absorbed by colon [26], which is associated with stimulation of sodium transport from the colon in several species including humans [27]. This effect may be particularly important in acute diarrheal diseases in colon and cause colonic dysfunction [28].…”