Effect of shock waves on macrophages: A possible role in tissue regeneration and remodeling

Sukubo, Naths Grazia; Tibalt, Elisabetta; Respizzi, Stefano; Locati, Massimo; d'Agostino, María Cristina

doi:10.1016/j.ijsu.2015.07.719

Cited by 66 publications

(50 citation statements)

References 29 publications

(38 reference statements)

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“…In some preclinical studies, LESW significantly decreased infiltration by inflammatory cells (neutrophils and macrophages) in burned skin or skin flaps, ischemic heart muscle and in the urinary bladder . LESW not only decreased the infiltration of macrophages, but also caused inhibition of M1 marker genes ( CD80 , COX‐2 , CCL5 ) in M1 macrophages, and a synergistic effect on M2 marker genes ( ALOX15 , MRC1 , CCL18 ) in M2 macrophages . This leads to a decreased pro‐inflammatory profile in M1 macrophages, and an increase in the presence of M2 macrophages with an anti‐inflammatory profile .…”

Section: Physical Characteristics Of Swmentioning

confidence: 97%

“…67,68 LESW not only decreased the infiltration of macrophages, but also caused inhibition of M1 marker genes (CD80, COX-2, CCL5) in M1 macrophages, and a synergistic effect on M2 marker genes (ALOX15, MRC1, CCL18) in M2 macrophages. 69 This leads to a decreased pro-inflammatory profile in M1 macrophages, and an increase in the presence of M2 macrophages with an antiinflammatory profile. 65,69,70 ESWT treatment is also associated with suppression of pro-inflammatory cytokines, chemokines, and MMPs.…”

Section: Anti-inflammationmentioning

confidence: 99%

“…69 This leads to a decreased pro-inflammatory profile in M1 macrophages, and an increase in the presence of M2 macrophages with an antiinflammatory profile. 65,69,70 ESWT treatment is also associated with suppression of pro-inflammatory cytokines, chemokines, and MMPs. Pro-inflammatory cytokines (IL-1a, IL-4, IL-6, IL-10, IL-12, IL-13 and INF-c) were significantly suppressed after ESWT in murine models.…”

Section: Anti-inflammationmentioning

confidence: 99%

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Potential applications of low‐energy shock waves in functional urology

2017

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Abbreviations & AcronymsAbstract: A shock wave, which carries energy and can propagate through a medium, is a type of continuous transmitted sonic wave with a frequency of 16 Hz-20 MHz. It is accompanied by processes involving rapid energy transformations. The energy associated with shock waves has been harnessed and used for various applications in medical science. High-energy extracorporeal shock wave therapy is the most successful application of shock waves, and has been used to disintegrate urolithiasis for 30 years. At lower energy levels, however, shock waves have enhanced expression of vascular endothelial growth factor, endothelial nitric oxide synthase, proliferating cell nuclear antigen, chemoattractant factors and recruitment of progenitor cells; shock waves have also improved tissue regeneration. Low-energy shock wave therapy has been used clinically with musculoskeletal disorders, ischemic cardiovascular disorders and erectile dysfunction, through the mechanisms of neovascularization, anti-inflammation and tissue regeneration. Furthermore, low-energy shock waves have been proposed to temporarily increase tissue permeability and facilitate intravesical drug delivery. The present review article provides information on the basics of shock wave physics, mechanisms of action on the biological system and potential applications in functional urology.

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Section: Physical Characteristics Of Swmentioning

confidence: 97%

Section: Anti-inflammationmentioning

confidence: 99%

Section: Anti-inflammationmentioning

confidence: 99%

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Potential applications of low‐energy shock waves in functional urology

2017

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“…ESWT influences the toll-like receptor 3 (TLR3) pathway [43], which plays a fundamental role in pathogen recognition and activation of innate immunity, so ESWT modulates the inflammatory responses related to immunology pathway [44][45][46][47][48][49].…”

Section: Eswt and Chronic Wound Healingmentioning

confidence: 99%

Extracorporeal Shock Wave Therapy in Chronic Wound Care

Carmignano¹

2020

Physical Therapy Effectiveness

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Chronic wounds are wounds, which failed healing and timely reparative process to produce anatomic and functional integrity over a period of 3 months. New physical therapy for chronic wounds is extracorporeal shock wave therapy (ESWT). ESWT generators can be focused, defocused, and radial. ESWT is non-invasiveness, low-associated complication rates, efficacy for indications refractory to other standards of practice, and cost-effectiveness.ESWT determines mechanotransduction that is possible as most of the cells of the body, thank to surface receptors and other transmission signals. In the specific field of ESWT, different biochemical substances are able to influence the processes of different cell lineages, besides to induce the formation of new small blood vessels. So, ESWT enhanced cell proliferation at the local wound tissue level, stimulated extracellular matrix metabolism, decreased apoptosis, and downregulated oxygen-mediated burst of leukocytes, probably stimulating homing and differentiation of stem cells with high tissue regenerating potential. From numerous experimental and clinical data, it is possible to conclude that ESWT would improve not only the wounds healing process but also the regeneration events.

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“…HIF-1α levels were significantly elevated in osteoblasts after in vitro stimulation [ 22 ] and ESWT-stimulated tissues showed higher vascularization both in animal and human trials [ 22 , 23 , 24 , 25 ]. Furthermore, ESWT was able to affect cellular inflammation by down-regulating leukocyte infiltration [ 26 ] and via modulation of macrophage activity [ 27 ]. By inhibition of NF-κB-associated proteins, such as iNOS, TNF-α, ICAM, VCAM, and COX-2, the local inflammatory response was shown to be affected by ESWT on the molecular level [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

In Vivo Modulation of Angiogenesis and Immune Response on a Collagen Matrix via Extracorporeal Shockwaves

Heimes

Wiesmann

Eckrich³

et al. 2020

IJMS

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The effective management of tissue integration and immunological responses to transplants decisively co-determines the success of soft and hard tissue reconstruction. The aim of this in vivo study was to evaluate the eligibility of extracorporeal shock wave therapy (ESWT) with respect to its ability to modulate angiogenesis and immune response to a collagen matrix (CM) for tissue engineering in the chorioallantoic membrane (CAM) assay, which is performed with fertilized chicken eggs. CM were placed on the CAM on embryonic development day (EDD) 7; at EDD-10, ESWT was conducted at 0.12 mJ/mm2 with 500 impulses each. One and four days later, angiogenesis represented by vascularized area, vessel density, and vessel junctions as well as HIF-1α and VEGF gene expression were evaluated. Furthermore, immune response (iNOS2, MMP-9, and MMP-13 via qPCR) was assessed and compared between ESWT- and non-ESWT-groups. At EDD-14, the vascularized area (+115% vs. +26%) and the increase in vessel junctions (+751% vs. +363%) were significantly higher in the ESWT-group. ESWT significantly increased MMP-9 gene expression at EDD-11 and significantly decreased MMP-13 gene expression at EDD-14 as compared to the controls. Using the CAM assay, an enhanced angiogenesis and neovascularization in CM after ESWT were observed. Furthermore, ESWT could reduce the inflammatory activity after a latency of four days.

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Effect of shock waves on macrophages: A possible role in tissue regeneration and remodeling

Abstract: Macrophage exposure to low energy SW dampens the induction of the pro-inflammatory profile characterizing M1 macrophages and promotes the acquisition of an anti-inflammatory profile synergizing with macrophage alternative activation.

Cited by 66 publications

References 29 publications

Potential applications of low‐energy shock waves in functional urology

Potential applications of low‐energy shock waves in functional urology

Extracorporeal Shock Wave Therapy in Chronic Wound Care

In Vivo Modulation of Angiogenesis and Immune Response on a Collagen Matrix via Extracorporeal Shockwaves

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