Effect of Shikonin on Human Breast Cancer Cells Proliferation and Apoptosis In Vitro

Hou, Yue; Guo, Tao; Wu, Chunfu; He, Xiang; Zhao, Ming

doi:10.1248/yakushi.126.1383

Cited by 42 publications

(36 citation statements)

References 12 publications

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“…Shikonin-rendered cell apoptosis through activation of a caspase-dependent pathway was found in HL60 human leukemia cells, 10) malignant melanoma cells, 13) Hela cells, 11) colorectal cancer, 17) and breast cancer. 30) Indeed, shikonin diminished the cell proliferation of 143B osteosarcoma cells in a dose-and time-dependent manner. Shikonininduced apoptosis in 143B osteosarcoma cells was evidenced by an increased sub-G1 population and enhanced DNA condensation.…”

Section: Discussionmentioning

confidence: 97%

Shikonin Induces Apoptosis through Reactive Oxygen Species/Extracellular Signal-Regulated Kinase Pathway in Osteosarcoma Cells

Chang

Huang

Chiang

et al. 2010

Biological & Pharmaceutical Bulletin

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Shikonin, a major ingredient in the Chinese traditional herb Lithospermum erythrorhixon, exhibits multiple biological functions including antimicrobial, anti-inflammatory, and antitumor effects. In this study, we delineated the molecular mechanisms of shikonin in the apoptosis of 143B osteosarcoma cells. Shikonin reduced the cell viability of 143B cells in a dose-and time-dependent manner. The IC 50 at 24 h and 48 h for 143B cells was 4.55 and 2.01 m mM, respectively. A significantly elicited hypodiploid cell population was found in cells treated with 2, 4, and 8 m mM shikonin for 24 h. Moreover, treatment with shikonin induced reactive oxygen species (ROS) generation, increased extracellular signal-regulated kinase (ERK) phosphorylation, decreased B-cell lymphoma-2 (Bcl2) expression, and was accompanied by poly(ADP-ribose) polymerase (PARP) cleavage. Pretreatment with the antioxidant agent N-acetyl cysteine (NAC) not only reversed shikonin-induced ROS generation but also significantly attenuated the cytotoxic effects of shikonin in 143B cells. Furthermore, NAC attenuated shikonin-induced ERK phosphorylation. Taken together, our results reveal that shikonin increased ROS generation and ERK activation, and reduced Bcl2, which consequently caused the cells to undergo apoptosis. Therefore, shikonin may be a promising chemotherapeutic agent for osteosarcoma treatment.

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Section: Discussionmentioning

confidence: 97%

Shikonin Induces Apoptosis through Reactive Oxygen Species/Extracellular Signal-Regulated Kinase Pathway in Osteosarcoma Cells

Chang

Huang

Chiang

et al. 2010

Biological & Pharmaceutical Bulletin

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“…Cell samples were incubated at 4°C in the dark for at least 15 m, and analyzed by a FACScan ‰ow cytometer (Becton Dickinson). 13) Data Analysis Results were expressed as mean ±SD. Statistical signiˆcance ( p＜0.05) was assessed by one-way ANOVA followed by least signiˆcant diŠerence method (SPSS12.0 software, SPSS, USA).…”

Section: Cell Culturementioning

confidence: 99%

Effect of Tetrandrine Combined with Epirubicin on the Growth of Human Breast Carcinoma Multidrug Resistance Cell Line

Hou

Guo

et al. 2008

YAKUGAKU ZASSHI

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Multidrug resistance presents a serious problem in cancer chemotherapy. Recent studies have shown that the multidrug resistance of tumor cells can be reversed by tetrandrine by potentiating the cytotoxicity of chemotherapeutic agents. However, whether tetrandrine has such potentiating eŠect on epirubicin has not been reported. Thus, the combined eŠect of tetrandrine and epirubicin on the growth of human breast carcinoma multidrug-resistant MCF-7/ADM cells was studied in the present study. It was shown that tetrandrine signiˆcantly potentiated the cytotoxicity of epirubicin. To examine the mechanism of the combined eŠect of tetrandrine and epirubicin on MCF-7/ADM cell growth, cell cycle progression was evaluated by using ‰ow cytometry. The combined use of tetrandrine and epirubicin caused an accumulation of cells at G 2 /M phase, accompanied with a concomitant decrement of cell number at G 0 /G 1 phase. The present study demonstrated for theˆrst time that tetrandrine potentiated the cytotoxcity of epirubicin on MCF-7/ADM cells. Cell cycle arrest at G 2 /M phase may contribute to the combined eŠect of tetrandrine and epirubicin.

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“…Han et al (2007) showed that shikonin stimulated cell death in MCF-7 and HEK293 cancer lines distinct from apoptosis but accompanied with (a) a morphology of necrotic cell death; (b) loss of plasma membrane integrity; (c) loss of mitochondrial membrane potentials; (d) activation of autophagy as a downstream consequence of cell death, but not a causal factor. Anticancer activitiy of shikonin and/or its derivatives has been indicated by other studies as well (Zhang et al 2005;Hou et al 2006). Acetylshikonin isolated from A. euchroma cell suspension cultures exhibited specific in vivo and in vitro antitumor effects (Xiong et al 2009 (1), alkannin (2), shikonin (3), catechin (4), isorhamnetin (5), hypericin (6), hyperforin (7), podophyllotoxin (8), emodin (9), rhein (10) Phytochem Rev (2011) 10:309-323 313 acetylshikonin inhibited K562 and HL-60 tumor growth (Xuan and Hu 2009).…”

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confidence: 77%

Anticancer potential of Himalayan plants

et al. 2010

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Plants used in traditional medicine have stood up to the test of time and contributed many novel compounds for preventive and curative medicine to modern science. India is sitting on a gold mine of well recorded and traditionally well practiced knowledge of herbal medicine. Specially, plants growing at high altitude in Himalayan pastures are time-honored sources of health and general well being of local inhabitants. As of today, Himalayan plants are a major contributor to the herbal pharmaceutical industry both of India and other countries. Plants growing at higher altitudes are subjected to an assault of diverse testing situations including higher doses of mutagenic UV-radiation, physiological drought, desiccation and strong winds. Plants interact with stressful environments by physiological adaptation and altering the biochemical profile of plant tissues and producing a spectrum of secondary metabolites. Secondary metabolites are of special interest to scientists because of their unique pharmacophores and medicinal properties. Secondary metabolites like polyphenols, terpenes and alkaloids have been reported to possess antimutagenic and anticancer properties in many studies. The fundamental aspiration of the current review is to divulge the antimutagenic/anticancer potential of five alpine plants used as food or medicine by the populations living at high altitudes.

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Effect of Shikonin on Human Breast Cancer Cells Proliferation and Apoptosis In Vitro

Cited by 42 publications

References 12 publications

Shikonin Induces Apoptosis through Reactive Oxygen Species/Extracellular Signal-Regulated Kinase Pathway in Osteosarcoma Cells

Shikonin Induces Apoptosis through Reactive Oxygen Species/Extracellular Signal-Regulated Kinase Pathway in Osteosarcoma Cells

Effect of Tetrandrine Combined with Epirubicin on the Growth of Human Breast Carcinoma Multidrug Resistance Cell Line

Anticancer potential of Himalayan plants

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