Effect of sheep and human follicular fluid on the maturation of sheep oocytes in vitro

Sun, Fangzhou; Holm, Preben Bach; Irvine, B.; Seamark, R.F.

doi:10.1016/0093-691x(94)90513-i

Cited by 27 publications

(10 citation statements)

References 26 publications

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“…Many studies have attempted to characterize the com¬ position and to examine the effect of the presence of follicular fluid during oocyte maturation on subsequent development (Andersen et al, 1976;Ayoub and Hunter, 1993;Lonergan et al, 1994a;Romero and Seidel, 1994;Sun et al, 1994;Sirard et al, 1995;Carolan et al, 1996). The ability of follicular fluid to support oocytes during IVM appears to be influenced by its origin.…”

mentioning

confidence: 97%

Characterization of calf follicular fluid and its ability to support cytoplasmic maturation of cow and calf oocytes

Khatir

Carolan²,

Lonergan³

et al. 1997

Reproduction

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The aims of the present study were to characterize the follicular fluid from prepubertal calf follicles of known size and quality and to study the ability of follicular fluid to support cytoplasmic maturation of calf and cow oocytes. Follicular fluid was obtained from 67 calf follicles classified according to size (S: small < 6 mm, M: medium 6-8 mm and L: large > 8 mm in diameter) and quality (HY: healthy, EA: early atretic and A: atretic). Quality was first determined by mitosis:pycnosis ratios in granulosa cell smears and confirmed by insulin-like growth factor binding protein (IGFBP) patterns. There was approximately 90% agreement between the two methods of follicle classification and on this basis the calf follicular fluid was pooled into nine groups. The accuracy of this pooling was confirmed by evaluation of oestradiol concentrations in the nine pools of follicular fluid using radio-immunoassay. Increases in follicle size were characterized by a decreased intensity of bands for IGFBP-2, IGFBP-5 and IGFBP-4, an increase in the proportion of healthy follicles and a decrease in the proportion of follicles in the early stages of atresia. This finding is in agreement with previously published results in cows. All classes of calf follicular fluid contained lower concentrations of oestradiol than previously reported for corresponding classes of cow follicular fluid. Cow oocytes were matured in M199 alone, or supplemented with 10% fetal calf serum (FCS), or 10% calf follicular fluid from one of three pools (LHY, LEA, LA), fertilized, and cultured for 8 days in synthetic oviduct fluid. Addition of FCS or calf follicular fluid to cow oocytes during in vitro maturation increased the yield of blastocysts on day 8 over the control (23%, 21/91), FCS (39%, 37/96, P < 0.05), LA (41% 21/52, P < 0.05), LEA (32%, 28/88), LHY (36%, 32/88), although not significantly in all cases. The rate of hatching of blastocysts was also improved: control (38%, 8/21), FCS (54%, 20/37), LA (62%, 13/21), LEA (75%, 21/28, P < 0.02), LHY (59% 19/32). In contrast, the addition of either FCS, calf follicular fluid or cow follicular fluid did not improve development of calf oocytes compared with the unsupplemented control. In conclusion, it is probable that serum and follicular fluid contain factors that stimulate the acquisition by oocytes, during maturation, of developmental competence and to which prepubertal oocytes are unable to respond. Specific receptors for these factors may develop only around puberty.

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mentioning

confidence: 97%

Characterization of calf follicular fluid and its ability to support cytoplasmic maturation of cow and calf oocytes

Khatir

Carolan²,

Lonergan³

et al. 1997

Reproduction

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“…The maturation media compositions used in the present study are based on previous research developed with abattoir‐derived sheep oocytes. The undefined medium is based on results, demonstrating that FF enhances the maturation and fertilizability of sheep follicular oocytes in vitro (Sun et al. 1994), and on additional results demonstrating the beneficial effects of FF and FSH on embryo developmental ability in sheep (Cognié 1999).…”

Section: Discussionmentioning

confidence: 99%

“…The maturation media compositions used in the present study are based on previous research developed with abattoir-derived sheep oocytes. The undefined medium is based on results, demonstrating that FF enhances the maturation and fertilizability of sheep follicular oocytes in vitro (Sun et al 1994), and on additional results demonstrating the beneficial effects of FF and FSH on embryo developmental ability in sheep (Cognie´1999). As far as we know, the defined medium used in this study has never been tested in oocytes aspirated from live ewes, although satisfactory in vitro production results have been described in abattoirderived goat and sheep oocytes (Cognie´et al 2002(Cognie´et al , 2004, as well as in oocytes aspirated from FSHstimulated goats (Locatelli et al 2008), confirming the efficiency of EGF (Guler et al 2000) and cysteamine (De Matos et al 1999Cognie´et al 2004;Shabankareh and Zandi 2010) as additives during in vitro maturation in the ovine species.…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…On the other hand, pioneer experimental results demonstrated that follicular fluid (FF) is a useful supplement in IVM medium as an adjunct to, rather than replacement for, the standard serum and hormone supplements (Sun et al 1994). It is known that FF contains high amounts of steroid hormones (Ainsworth et al 1980), while concentrations of gonadotropins are similar to those found in plasma (Fortune and Hansel 1985).…”

Section: Introductionmentioning

confidence: 99%

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The Efficiency of In vitro Ovine Embryo Production Using an Undefined or a Defined Maturation Medium is Determined by the Source of the Oocyte

Cocero

Alabart

Hammami

et al. 2010

Reprod Domestic Animals

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In vitro oocyte maturation can be influenced by oocyte source and maturation media composition. The aim of the present study was to compare the efficiency of a defined in vitro maturation medium (TCM199 supplemented with cysteamine and epidermal growth factor; Cys + EGF) with an undefined medium (TCM199 supplemented with follicle-stimulating hormone and follicular fluid; FSH + FF) for in vitro production (IVP) of ovine embryos, using oocytes obtained by laparoscopic ovum pick-up from FSH-stimulated [n=11; 158 cumulus-oocyte complexes (COCs)] and non-stimulated (n=16; 120 COCs) live ewes, as well as abattoir-derived oocytes (170 COCs). The produced blastocysts were vitrified and some of them were transferred to synchronized recipients. The best and the worst final yields of embryo IVP observed in this study were obtained using oocytes from FSH-stimulated ewes matured in FSH + FF (41.3%; 33/80) and in Cys + EGF (19.2%; 15/78) medium, respectively (p<0.01). No significant differences between both media were attained in the blastocyst development rate or in the final yield of embryo IVP using oocytes from non-stimulated ewes or abattoir-derived oocytes. The overall in vivo survival rate of the transferred vitrified blastocysts was 13.1% (8/61), without significant differences between oocyte sources or maturation media. In conclusion, under the experimental conditions of the present study, TCM199 supplemented with cysteamine and EGF is a convenient defined maturation medium for IVP of embryos from oocytes of live non-stimulated ewes or from oocytes of abattoir-derived ovaries. However, the best final yield of embryo IVP observed in this study was attained when oocytes came from FSH-stimulated donors and TCM199 was supplemented with FSH and follicular fluid.

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Effect of follicle size and quality on the ability of follicular fluid to support cytoplasmic maturation of bovine oocytes

et al. 1996

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Effect of sheep and human follicular fluid on the maturation of sheep oocytes in vitro

Cited by 27 publications

References 26 publications

Characterization of calf follicular fluid and its ability to support cytoplasmic maturation of cow and calf oocytes

Characterization of calf follicular fluid and its ability to support cytoplasmic maturation of cow and calf oocytes

The Efficiency of In vitro Ovine Embryo Production Using an Undefined or a Defined Maturation Medium is Determined by the Source of the Oocyte

Effect of follicle size and quality on the ability of follicular fluid to support cytoplasmic maturation of bovine oocytes

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