Effect of serum on secreteion of prostacyclin and endothelin-1 by decidual endothelial cells from normal and preeclamptic pregnancies

Gallery, Eileen D.M.; Rowe, Janet; Campbell, Suzanne; Hawkins, Therese

doi:10.1016/0002-9378(95)90366-6

Cited by 28 publications

(12 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Endothelial cells were isolated from macaque uterine tissue following the method described by [33]. The cells were PECAM-1 and Factor VIII-positive.…”

Section: Endothelial Cellsmentioning

confidence: 99%

Macaque trophoblast migration is regulated by RANTES

Thirkill¹,

Lowe²,

Vedagiri³

et al. 2005

Experimental Cell Research

View full text Add to dashboard Cite

“…Endothelial cells were isolated from macaque uterine tissue following the method described by [33]. The cells were PECAM-1 and Factor VIII-positive.…”

Section: Endothelial Cellsmentioning

confidence: 99%

Macaque trophoblast migration is regulated by RANTES

Thirkill¹,

Lowe²,

Vedagiri³

et al. 2005

Experimental Cell Research

View full text Add to dashboard Cite

“…In cultured ECs, exposure to serum from PE women results in increased expression of NOS and NO, release of PDGF, fibronectin which promotes platelet aggregation, increased secretion of ET-1, induction of oxygen radicals, and inhibition of PGI 2 production (Taylor et al1991a,b, Baker et al1995, 1996; Davidge et al1995, Gallery et al1995). The release of some of these cellular mediators is partly due to increased ECs [Ca 2+ ] i upon exposure to PE serum.…”

Section: Introductionmentioning

confidence: 99%

Vascular and Cellular Calcium in Normal and Hypertensive Pregnancy

Adamová¹,

Ozkan²,

Khalil³

2009

CCP

View full text Add to dashboard Cite

Normal pregnancy is associated with significant hemodynamic changes in the cardiovascular system in order to meet the metabolic demands of mother and fetus. These changes include increased cardiac output, decreased vascular resistance, and vascular remodeling in the uterine and systemic circulation. Preeclampsia (PE) is a major complication of pregnancy characterized by proteinuria and hypertension. Several risk factors have been implicated in the pathogenesis of PE including genetic and dietary factors. Ca 2+ is an essential dietary element and an important regulator of many cellular processes including vascular function. The importance of adequate dietary Ca 2+ intake during pregnancy is supported by many studies. Pregnancy-associated changes in Ca 2+ metabolism and plasma Ca 2+ have been observed. During pregnancy, changes in intracellular free Ca 2+ concentration ([Ca 2+ ] i ) have been described in red blood cells, platelets and immune cells. Also, during pregnancy, an increase in [Ca 2+ ] i in endothelial cells (EC) stimulates the production of vasodilator substances such as nitric oxide and prostacyclin. Normal pregnancy is also associated with decreased vascular smooth muscle (VSM) [Ca 2+ ] i and possibly the Ca 2+ -sensitization pathways of VSM contraction including protein kinase C, Rho-kinase, and mitogen-activated protein kinase. Ca 2+ -dependent matrix metalloproteinases could also promote extracellular matrix degradation and vascular remodeling during pregnancy. Disruption in the balance between dietary, plasma and vascular cell Ca 2+ may be responsible for some of the manifestation of PE including procoagulation, decreased vasodilation, and increased vasoconstriction and vascular resistance. The potential benefits of Ca 2+ supplements during pregnancy, and the use of modulators of vascular Ca 2+ to reduce the manifestations of PE in susceptible women remain an important area for experimental and clinical research.

show abstract

“…We found that HUVECs incubated with serum from healthy pregnant women produced less LPOs than cells incubated with serum from pre-eclamptic women or healthy non-pregnant women ; however, amounts of GSH were similar. Many groups have evaluated the effects of serum from pre-eclamptic women on endothelial cells in culture, and have reported alterations in the production of vasoactive substances [6][7][8][9][10]. To our knowledge, this is the first study in which the production of LPOs and GSH has been measured in endothelial cells after incubation with serum.…”

Section: Discussionmentioning

confidence: 89%

“…This could promote a cycle of events that ultimately compromises the vasodilatory, anti-aggregatory and barrier function of the vascular endothelium [3][4][5]. Indeed, studies investigating the effects of serum or plasma from women with preeclampsia on endothelial cells have shown alterations in the secretion of endothelium-derived vasoactive substances [6][7][8][9][10].…”

Section: Introductionmentioning

confidence: 96%

Serum from healthy pregnant women reduces oxidative stress in human umbilical vein endothelial cells

et al. 2002

View full text Add to dashboard Cite

This study was conducted to compare the effects of serum from healthy pregnant women and that from pregnant women with pre-eclampsia on oxidative stress in endothelial cells in culture. Human umbilical vein endothelial cells (HUVECs) were incubated with serum from 18 pre-eclamptic, 18 healthy pregnant and 18 healthy non-pregnant women for 24 h. The levels of reduced glutathione (GSH) and lipid peroxides (LPOs) were measured in endothelial cell lysates. Measurement of malondialdehyde in combination with 4-hydroxyalkenals has been used as an indicator of LPOs. Serum from healthy pregnant women decreased significantly the LPO content in HUVECs in comparison with serum from pre-eclamptic women and healthy non-pregnant women (30.7+/-6.6 compared with 39.3+/-10.9 and 41.0+/-12.7 pmol/mg of protein respectively; P<0.003 and P<0.01 respectively). No differences in GSH content between the three groups (18.3+/-2.1 nmol/mg of protein for healthy pregnant, 19.2+/-3.3 nmol/mg for pre-eclamptic and 18.3+/-2.0 nmol/mg for healthy non-pregnant women) were found. Thus serum from normal pregnant women contains a factor(s) that decreases oxidative stress in human endothelial cells. This mechanism might be altered in pre-eclampsia.

show abstract

Effect of serum on secreteion of prostacyclin and endothelin-1 by decidual endothelial cells from normal and preeclamptic pregnancies

Cited by 28 publications

References 12 publications

Macaque trophoblast migration is regulated by RANTES

Macaque trophoblast migration is regulated by RANTES

Vascular and Cellular Calcium in Normal and Hypertensive Pregnancy

Serum from healthy pregnant women reduces oxidative stress in human umbilical vein endothelial cells

Contact Info

Product

Resources

About