Effect of serum concentration, method of trypsinization and fusion/activation utilizing transfected fetal cells to generate transgenic dairy goats by somatic cell nuclear transfer

Melican, D.; Butler, R.; Hawkins, N.; Chen, LiHow; Hayden, Evelyn; Destrempes, Margaret M.; Williams, Jennifer L.; Lewis, Tanya; Behboodi, E.; Ziomek, Carol A.; Meade, Harry; Echelard, Yann; Gavin, W.

doi:10.1016/j.theriogenology.2004.05.029

Cited by 36 publications

(17 citation statements)

References 37 publications

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“…Therefore, because no important difference was observed between the different characteristics of serum-starved and confluent cells in this study (Fig. 6), and considering the common trend of using serum starved cells for SCNT (Melican et al, 2005), the serum starvation method of cell cycle synchronization was used for providing G0/G1 cell population.…”

Section: In Vitro Maturationmentioning

confidence: 89%

“…Several lines of evidence indicate that the goat (Capra hircus) is the ideal species for transgenic production of recombinant proteins due to its unique advantages over cattle and sheep (Baguisi et al, 1999;Reggio et al, 2001;Behboodi et al, 2004;Lan et al, 2006;Melican et al, 2005). Therefore, it is expected that the demand for production of transgenic herds of goat with the use of a simple, fast, and efficient method of cloning will increase.…”

Section: Introductionmentioning

confidence: 99%

“…Although the first successful report of goat cloning turns back to 1999 (Baguisi et al, 1999), there are obstacles in the field of goat in vitro cloning and embryo culture that hamper 1 the broad application of this valuable species for transgenic purposes: (1) goat SCNT still practiced with the labor-intensive and time-consuming zona-intact method (Baldassarre et al, 2004a); (2) the majority of goat SCNT studies have used ovulated or OPU-derived oocytes, which make goat cloning expensive and demanding (Baldassarre and Karatzas, 2004;and (3) in most reports, reconstructed goat embryos have been either cultured in vitro as short as possible (up to the eight-cell stage) (Baguisi et al, 1999;Behboodi et al, 2004;Chen et al, 2007;Keefer et al, 2001;Lan et al, 2006;Melican et al, 2005;Ohkoshi et al, 2003;Reggio et al, 2001) or temporarily incubated in an intermediate oviduct (Behboodi et al, 2004;Xu et al, 2008;Zou et al, 2001) to avoid the abnormal/poor embryo development that has been associated with in vitro culture of goat embryos. Of note, the two teams who transferred in vitro developed morulae-blastocyst have experienced disappointing results.…”

Section: Introductionmentioning

confidence: 99%

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Development of an Optimized Zona-Free Method of Somatic Cell Nuclear Transfer in the Goat

Nasr-Esfahani

Hosseini

Hajian

et al. 2011

Cellular Reprogramming

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The purpose of this study was to develop an improved zona-free method of goat somatic cell nuclear transfer (SCNT) that has both ease of operation and efficiency. The main steps involved were: (1) optimization of in vitro oocyte maturation, (2) parthenogenetic activation of zona-free oocytes, (3) SCNT of zona-free anaphase II-telophase II (AII-TII) oocytes that subverted the need for long term UV-exposure of the oocytes, and (4) in vitro culture of groups of cloned embryos in wells in a highly efficient continuous serum-free embryo medium to the blastocyst stage before transfer to the recipients. Percentages of transgenic blastocyst production were 22.3 and 33.1% for adult and fetal cell lines, respectively. After transfer of cloned and transgenic blastocysts, 28.6 and 36.4% of the recipients were confirmed pregnant and 75 and 33.3% of the pregnancies resulted in the delivery of viable offspring, respectively. To our knowledge, this is the first report of successful live and survived birth of cloned and transgenic offspring through a whole procedure of in vitro oocyte maturation and embryo development to the blastocyst stage, and in this study the in vitro efficiencies of cloned and transgenic embryo production were higher than the available reports.

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Section: In Vitro Maturationmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Development of an Optimized Zona-Free Method of Somatic Cell Nuclear Transfer in the Goat

Nasr-Esfahani

Hosseini

Hajian

et al. 2011

Cellular Reprogramming

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“…The goat (Capra hircus) is an ideal species for production of transgenic animals (Baguisi et al, 1999;Baldassarre et al, 2004;Behboodi et al, 2004;Lan et al, 2006;Melican et al, 2005;Nasr-Esfahani et al, 2011;Reggio et al, 2001). Different culture media have been used for in-vitro culture of the reconstructed goat embryos, including Charles Rosenkrans medium (CR1) (Chen et al, 2007;Guo et al, 2002;Lan et al, 2006), tissue culture medium (TCM)-199 (Baguisi et al, 1999) and synthetic oviductal fluid (SOF) (Melican et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

“…Different culture media have been used for in-vitro culture of the reconstructed goat embryos, including Charles Rosenkrans medium (CR1) (Chen et al, 2007;Guo et al, 2002;Lan et al, 2006), tissue culture medium (TCM)-199 (Baguisi et al, 1999) and synthetic oviductal fluid (SOF) (Melican et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Contrasting effects of G1.2/G2.2 and SOF1/SOF2 embryo culture media on pre- and post-implantation development of non-transgenic and transgenic cloned goat embryos

Hosseini

Hajian

Ostadhosseini

et al. 2015

Reproductive BioMedicine Online

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Developmental expression of pluripotency determining factors in caprine embryos: Novel pattern of NANOG protein localization in the nucleolus

Pant

Schiffmacher

et al. 2006

Molecular Reproduction Devel

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Transcription factors, POU5F1/OCT4 and NANOG, whose expression is restricted to the inner cell mass (ICM) in mouse and human blastocysts, are used to characterize undifferentiated embryonic stem cells (ESC) in vitro. However, POU5F1 may not be a useful marker in domestic animals due to its expression in both ICM and trophectoderm (TE), while NANOG mRNA and protein expression have only been described fully in mice. In an effort to identify ESC markers for domestic animals, expression patterns of NANOG, POU5F1, and the cell surface markers (SSEA1, SSEA4, TRA-1-60, TRA-1-81) were examined in preimplantation goat embryos, a species that has proven to be a superior choice for the production of transgenic proteins in milk (biopharming). Our results indicate that while goat embryos express POU5F1, SSEA1, and SSEA4 proteins, their expression is not strictly restricted to the ICM. In a unique staining pattern, NANOG protein was localized to the nucleoplasm and nucleoli in ICM cells, but was localized strictly to nucleoli in TE. This pattern may reflect down-regulation of protein by sequestration/degradation utilizing a nucleolar mechanism known to operate in stem cells. Furthermore, NANOG mRNA in TE was also significantly down-regulated as compared with that in ICM. Taken together, this novel expression pattern of NANOG in goat preimplantation embryos suggests that NANOG could serve as marker of pluripotency in goats and may be useful in derivation and characterization of caprine ESC. This study is the first to characterize both NANOG mRNA and protein expression in any species other than the mouse.

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Effect of serum concentration, method of trypsinization and fusion/activation utilizing transfected fetal cells to generate transgenic dairy goats by somatic cell nuclear transfer

Cited by 36 publications

References 37 publications

Development of an Optimized Zona-Free Method of Somatic Cell Nuclear Transfer in the Goat

Development of an Optimized Zona-Free Method of Somatic Cell Nuclear Transfer in the Goat

Contrasting effects of G1.2/G2.2 and SOF1/SOF2 embryo culture media on pre- and post-implantation development of non-transgenic and transgenic cloned goat embryos

Developmental expression of pluripotency determining factors in caprine embryos: Novel pattern of NANOG protein localization in the nucleolus

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