Effect of serum components on the physico-chemical properties of cationic lipid/oligonucleotide complexes and on their interactions with cells

Zelphati, Olivier; Uyechi, L S; Barron, Lee G.; Szoka, Francis C.

doi:10.1016/s0005-2760(97)00169-0

Cited by 305 publications

(219 citation statements)

References 32 publications

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“…plex and substrate. Similarly, complexes were expected to adsorb to serum-modified substrates due to their known interaction with serum components (Moret et al, 2001;Zelphati et al, 1998). We propose that the molecular-scale design of the DNA complex and the substrate can be employed to regulate the molecular interactions (e.g., electrostatic, van der Waals, hydrophobic) and will lead to optimal transfection by a substrate-mediated approach.…”

Section: Discussionmentioning

confidence: 99%

Gene delivery through cell culture substrate adsorbed DNA complexes

Bengali

Pannier

Segura

et al. 2005

Biotech & Bioengineering

130

187

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Efficient gene delivery is a fundamental goal of biotechnology and has numerous applications in both basic and applied science. Substrate-mediated delivery and reverse transfection enhance gene transfer by increasing the concentration of DNA in the cellular microenvironment through immobilizing a plasmid to a cell culture substrate prior to cell seeding. In this report, we examine gene delivery of plasmids that were complexed with cationic polymers (polyplexes) or lipids (lipoplexes) and subsequently immobilized to cell culture or biomaterial substrates by adsorption. Polyplexes and lipoplexes were adsorbed to either tissue culture polystyrene or serumadsorbed tissue culture polystyrene. The quantity of DNA immobilized increased with time of exposure, and the deposition rate and final amount deposited depended upon the properties of the substrate and complex. For polyplexes, serum modification enhanced reporter gene expression up to 1500-fold relative to unmodified substrates and yielded equivalent or greater expression compared to bolus delivery. For lipoplexes, serum modification significantly increased the number of transfected cells relative to unmodified substrates yet provided similar levels of expression. Immobilized complexes transfect primary cells with improved cellular viability relative to bolus delivery. Finally, this substrate-mediated delivery approach was extended to a widely used biomaterial, poly(lactide-coglycolide). Immobilization of DNA complexes to tissue culture polystyrene substrates can be a useful tool for enhancing gene delivery for in vitro studies. Additionally, adapting this system to biomaterials may facilitate application to fields such as tissue engineering.

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Section: Discussionmentioning

confidence: 99%

Gene delivery through cell culture substrate adsorbed DNA complexes

Bengali

Pannier

Segura

et al. 2005

Biotech & Bioengineering

130

187

View full text Add to dashboard Cite

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“…Serum-induced inhibition of lipoplex-mediated transfection has been often reported. [25][26][27][28] However, the transfection ability of the SucPG-complex did not decrease even at a serum concentration of 40%. In contrast, the transfection activity of the plain lipoplex decreased slightly as serum concentration of the medium increased up to 20%, although its serum sensitivity was not significant.…”

Section: Figure 5 Luciferase Activity Of Hela Cells Treated With Tranmentioning

confidence: 92%

Novel gene delivery systems: complexes of fusigenic polymer-modified liposomes and lipoplexes

et al. 2001

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We have previously developed the succinylated poly(glycidol)-modified liposome which becomes fusigenic under weakly acidic condition. In this report, we describe that complexation of this pH-sensitive, fusigenic liposome with a lipoplex consisting of 3␤-(N-(NЈ,NЈ-dimethylaminoethane) carbamoyl)cholesterol, dioleoylphosphatidylethanolamine and plasmid DNA gives efficient gene delivery systems. In this study, we prepared the complexes, which are termed SucPG-complexes, with a positively or negatively charged surface by mixing the lipoplex with varying amounts of the SucPG-modified liposomes. The positively charged SucPGcomplexes either bearing or not bearing a cell-specific

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“…After intravenous injection of lipoplex, plasmid DNA would be dissociated from the cationic liposomes in the blood circulation by the interaction with biological components, such as blood constituents and proteoglycans. 25,27,28 Free DNA dissociated from cationic liposomes is susceptible to degradation by nucleases in the blood and the degradation products are excreted into the urine via the kidney and distributed throughout the body. Kawabata et al 29 have demonstrated these processes after i.v.…”

Section: Increases In the Liver And Spleen After Lipoplex Injection Amentioning

confidence: 99%

The role of tissue macrophages in the induction of proinflammatory cytokine production following intravenous injection of lipoplexes

et al. 2002

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Recent studies have demonstrated that intravenous administration of a plasmid DNA-cationic liposome complex (lipoplex) induced significant proinflammatory cytokine production in blood and inhibited transgene expression in pulmonary endothelial cells. In this study, we examined the effects of gadolinium chloride (GdCl 3 ) pretreatment on the biodistribution and induction of proinflammatory cytokine production and transgene expression after intravenous injection of a lipoplex in mice. GdCl 3 is known to transiently deplete liver Kupffer cells and spleen macrophages after intravenous administration. Intravenous administration of a lipoplex triggers high levels of proinflammatory cytokine production, such as TNF-␣, IFN-␥ and IL-12 in serum and

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Effect of serum components on the physico-chemical properties of cationic lipid/oligonucleotide complexes and on their interactions with cells

Cited by 305 publications

References 32 publications

Gene delivery through cell culture substrate adsorbed DNA complexes

Gene delivery through cell culture substrate adsorbed DNA complexes

Novel gene delivery systems: complexes of fusigenic polymer-modified liposomes and lipoplexes

The role of tissue macrophages in the induction of proinflammatory cytokine production following intravenous injection of lipoplexes

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