Effect of serogroup, surface material and disinfectant on biofilm formation by avian pathogenic Escherichia coli

Pseudomonas grimontii biofilm protects food contact surfaces from Escherichia coli colonization

“…Besides the particular bacterial species or strain, biofilm onset and resistance in food processing environments may also be affected by the properties of the contact surface (Lemos, Gomes, Mergulhão, Melo, & Simões, 2015;Moreira, Gomes, Simões, Melo, & Mergulhão, 2015;Oosterik, Tuntufye, Butaye, & Goddeeris, 2014). In food plants, it is common to find critical zones, such as crevices, corners, joints and valves, with low fluid velocities, making these zones suitable places for biofilm development (Lemos et al, 2015).…”

Virulence Potential, Biofilm Formation, and Disinfectants Control of Escherichia coli from Raw Milk Bulk Tanks in the Southeast of Brazil

“…The outcomes were then classified into two groups: biofilm formers and non-biofilm formers. This classification was achieved by computing the cutoff point as follows: the mean OD540 value of the negative control strain, augmented by three times the standard deviation, was calculated across the three replicates per plate to establish the cutoff OD (ODc) specific to each plate [7]. For the tests involving stainless steel and silicone, we utilized coupons that were meticulously washed, dried, and sterilized via autoclaving.…”

“…This arrangement was established in triplicate, and each well received a 300 µL aliquot from a 10 8 CFU dilution. Subsequently, the plate was incubated at 35 • C for 48 h, as per the method outlined by Oosterik and colleagues [7]. After the incubation period, the coupons were transferred to a new plate.…”