Effect of Sericin and Curcumin Mixture on Hematological Alternations Associated with Carrageenan Induced Paw Edema in Mice

Abstract

Inflammatory diseases can be life threatening, if persist for a longer time. In the current study, we aimed to investigate the effect of sericin and curcumin either alone or together on the reversal of hematological changes induced by carrageenan in the mice model. Mice were induced edema via λ-carrageenan (50µl/ mice) and different concentrations of sericin (0.03 mg/ml, 0.06 mg/ml and 0.09 mg/ml) and curcumin (1%, 2%, and 3%) were administered to the respective groups, then blood was collected for hematological analysis to observe the mean count of erythrocytes, hemoglobin, platelets, white blood cells (neutrophils, monocytes, eosinophils and basophils) in control and experimental groups. Based on the preliminary results a mixture of best concentration of sericin and curcumin was successfully achieved and was used in further studies. In the group that was given carrageenan alone, the number of erythrocytes, hemoglobin and platelets were decreased, whereas count of white blood cells was increased significantly. The effect of sericin and curcumin was dose dependent. The highest concentrations of sericin (0.09 mg/ml) and curcumin (3%) reversed the blood parameters close to the normal. The hematological studies for this mixture formulation of sericin 0.09 mg/ml sericin and 3% curcumin were reported for the first time and they significantly (p< 0.05) balanced all blood cells.Novelty Statement | This research article provides a novel mechanistic study to control inflammatory pro-cesses through topical application of natural non-toxic substances such as sericin and curcumin.