Effect of sensory stimulation on the uptake and incorporation of radioactive lysine into protein of mouse brain and liver

Polyneuropathies and CNS Protein Metabolism. Ii. Changes in the Incorporation Rate of Leucine During Acrylamide Intoxication

“…The in uiuo incorporation rates of leucine into proteins of the spinal cord, of brain stem and of heart muscle were measured using a pulse of tritiated leucine by intraperitoneal injection after which a rather uniform labelling would be expected throughout the organism (Figure 1, Table 1) (Rees, Brogan, Entingh, Dunn, Shinkman, Damstra-Entingh, Wilson & Glassman, 1974). A relatively short incorporation period (20 min) was chosen for two reasons: (a) uptake and metabolism of the radioactive precursor in the tissue was fast (Schotman, Gispen, Jansz & De Wied, 1972;Schotman, Gipon & Gispen, 1974) and (b) incorporation was taken as measure of synthesis rather than turn-over rate of proteins.…”

Influence of triiodothyronine on the amino acid uptake of brain and spinal cord in normal and spinal hemisected adult rats

“…This depression was produced by increases in the soluble fraction radioactivity. The increased soluble fraction in early time intervals may be related to stress of injection in normal animals [Rees et al, 1974;Rees and Dunn, 1977;Semiginovsky et al, 19741 or may be related to the ability of T3 to facilitate amino acid transport [Goldfine et al, 1975a, b;19761 either in relation to the peripheral uptake of precursor or its entry into brain. The decrease in relative radioactivity in normals in early postinjection intervals is in agreement with autoradiographic data from normal sensory ganglion cells in T3-treated rats [Cook and Kiernan, 19761. In the present data, brain radioactivity returned to approximately normal untreated levels at 7-14 days as in autoradiographic studies [Cook and Kiernan, 19761. The results from T3 treatment in normal animals suggest that T3 can affect and stimulate amino acid incorporation into protein in the adult rat central nervous system from a peripheral injection.…”

Incorporation of [3H] lysine into the brain and spinal cord of the Cebus (Cebus appella) and Rhesus (Macacca mulata) monkeys

“…Of particular interest has been the protein synthesis of the CNS as reflected in the uptake of radioactive amino acid precursors. There has been considerable research on the incorporation of amino acids into protein in the brain and spinal cord compared t o that of other body tissue in mice (Semiginovsky and Jakoubek, 1971;Rees e t al., 1974) and in rats (Roberts e t al., 1970;Waelsch, 1957;Pakkenberg et al, 1965). In general, whole brain homogenates are used in these studies with little regard t o the heterogeneity of the brain as a n organ with a preponderance of various functional areas.…”