Effect of seminal plasma on functional integrity of rabbit sperm membranes during storage at 4ºC or freezing.

Aksoy, Melih; Lehimcioğlu, Necdet Cankat; Akman, Orhan

doi:10.4995/wrs.2008.642

Cited by 15 publications

(16 citation statements)

References 16 publications

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“…Sperm cryopreservation remains suboptimal in this species (Castellini et al 2006), and AI with cryopreserved rabbit sperm is actually only utilized for experimental or genetic resource bank purposes, because of the lower fertility and ⁄ or prolificacy achieved with the use of frozen-thawed sperm than fresh sperm (Moce´and Vicente 2009). The fertility loss with the use of cooledstored rabbit semen older than 24-48 h may be the consequence of deterioration in semen quality observed over storage time (Roca et al 2000;Lo´pez-Gatius et al 2005;Aksoy et al 2008;Iaffaldano et al 2010). This is consistent with researches showing a positive relationship between rabbit in vitro semen characteristics and in vivo reproductive performances (Brun et al 2002;Lavara et al 2005).…”

Section: Introductionsupporting

confidence: 86%

Effect of Chilling Temperature on the Long‐Term Survival of Rabbit Spermatozoa held Either in a Tris‐Based or a Jellified Extender

Rosato

Iaffaldano

2010

Reprod Domestic Animals

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As the preservation of the fertilizing capacity of rabbit spermatozoa for several days after semen collection remains a major target for the artificial insemination programs of rabbit breeding, a study was conducted to compare the efficacy of 5 or 15°C as holding temperature in lengthening the preservability of rabbit semen quality during 192 h of storage both in a solid (Cunigel) and a liquid (Tris-Citric acid-Glucose; TCG) extender. Six pooled semen samples (two ejaculates/male; two-three males/pool) were taken and made four aliquots: two aliquots were tenfold diluted with the TCG extender, whereas the other two were tenfold diluted with the Cunigel extender. One aliquot per diluent was stored at 5°C and the second one at 15°C. Sperm motility (light microscope), viability (SyBr-PI staining), plasma membrane functional integrity (Hypo-osmotic swelling test) and acrosome integrity (PSA-FITC staining) were recorded at 0, 48, 120 and 192 h of storage. In liquid-stored spermatozoa, mass motility and viability were significantly higher (p ≤ 0.05) in samples stored at 5°C than at 15°C at all the storage times; at 5°C resulted also higher (p ≤ 0.05) the percentages of both forward motility at 48 h and sperm functional integrity at 120 and 192 h of storage, whereas chilling temperature did not affect acrosome integrity. With the Cunigel extender, all the semen qualitative parameters were significantly higher in sample stored at 5 than 15°C over storage time (p ≤ 0.05); only acrosome integrity at 192 h was not different according to the chilling temperatures. In conclusion, 5°C were better than 15°C for the long-term storage of rabbit semen both in the TCG and Cunigel extender.

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Section: Introductionsupporting

confidence: 86%

Effect of Chilling Temperature on the Long‐Term Survival of Rabbit Spermatozoa held Either in a Tris‐Based or a Jellified Extender

Rosato

Iaffaldano

2010

Reprod Domestic Animals

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“…The effect of SP on the survival of ejaculated spermatozoa after cryopreservation has been studied using different plasma removal techniques, such as semen filtration (Graham and Graham 1990; Cisale et al. 2001; Aksoy et al. 2008), dialysis (Schmehl et al.…”

Section: Effect Of Seminal Plasma On Sperm Function After Cryopreservmentioning

confidence: 99%

Seminal Plasma Proteins and Sperm Resistance to Stress

Muiño‐Blanco

Pérez‐Pé

Cebrián‐Pérez

2008

Reprod Domestic Animals

135

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The role of seminal plasma (SP) in mammalian sperm function remains largely a matter of speculation as both inhibitory and stimulating effects have been found. Specific components of SP, particularly proteins, are adsorbed onto the surface of ejaculated sperm as they pass through the male and female reproductive tracts. These sperm coating components seem to have the important function of maintaining the stability of the membrane up to the process of capacitation (decapacitation factors). Therefore, they must be removed, modified or masked before the spermatozoa undergo the acrosome reaction, an essential process for successful fertilization. It is well known that low temperatures alter the function of spermatozoa. Cold shock results in the destabilization of sperm membranes and impairment of sperm function, and it is also well known that ram spermatozoa are more sensitive to cold-shock stress than those of other species. The addition of SP proteins to spermatozoa before and/or after cooling is able to minimize cryoinjury effects. The major proteins in ram SP which are able to protect and repair the cold-shock damage to sperm contain fibronectin-II domains. The significance of this domain and the role of these proteins in sperm capacitation and gamete interaction are discussed.

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“…Unfortunately, storage of rabbit semen for longer than 24 to 48 h causes deterioration of semen quality with a decrease in fertility (Roca et al, 2000;López-Gatius et al, 2005;Aksoy et al, 2008;Rosato and Iaffaldano, 2011). Therefore, extending the interval of liquid semen storage beyond 48 h or freezing the semen, remains one of the major goals in the meat rabbit industry.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Different Extenders on the Preservability of Rabbit Semen Stored at 5°C for 72 Hours

Iorio¹,

Manchisi²,

Rocco³

et al. 2014

Italian Journal of Animal Science

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This study was designed to compare Cortalap ® extender with tris-citric acid-glucose (TCG), Lepus ® and Merk III ® , to assess the in vitro preservability of rabbit spermatozoa stored for 72 h at 5°C. The best extender identified in vitro was then compared with fresh semen to determine fertility and prolificacy rates in vivo. Eight semen pools were split into four subsamples and each of them was diluted to a ratio of 1:10 (volume:volume) with four different extenders (Lepus ® , Cortalap ® , TCG and Merk III ® ) and stored at 5°C for 72 h. Sperm motility, viability, sperm membrane and acrosome integrity were evaluated in both fresh and chilled semen at 4, 24, 48 and 72 h of storage. The results showed that Cortalap ® was the best extender to preserve the quality of rabbit semen in vitro over 72 h in comparison with the other extenders (P<0.05). Then, Cortalap ® was used for an artificial insemination (AI) trial to be compared with fresh semen. Two groups of does (n=30 each) were inseminated with fresh and chilled semen. The fertility and prolificacy rates for the does inseminated with chilled semen were significantly lower compared with those inseminated with fresh semen (P<0.05). In conclusion, although Cortalap ® proved to be the best extender to preserve semen quality in vitro after 72 h at 5°C, the in vivo results showed that its use is not recommended in AI programmes.

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Effect of seminal plasma on functional integrity of rabbit sperm membranes during storage at 4ºC or freezing.

Cited by 15 publications

References 16 publications

Effect of Chilling Temperature on the Long‐Term Survival of Rabbit Spermatozoa held Either in a Tris‐Based or a Jellified Extender

Effect of Chilling Temperature on the Long‐Term Survival of Rabbit Spermatozoa held Either in a Tris‐Based or a Jellified Extender

Seminal Plasma Proteins and Sperm Resistance to Stress

Comparison of Different Extenders on the Preservability of Rabbit Semen Stored at 5°C for 72 Hours

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