Effect of semen thaw method on conception rate in four large commercial dairy heifer herds

Kaproth, M.; Rycroft, H.; Gilbert, G.R.; Abdel-Azim, G.; Putnam, B.F.; Schnell, Susanne; Everett, R.W.; Parks, JE

doi:10.1016/j.theriogenology.2004.11.001

Cited by 17 publications

(16 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A significant increase in the conception rate (35 vs. 27%) was reported when thawing occurred in warm-water (33-35°C) as opposed to air (Dejarnette and Marshall, 2005). Kaproth et al (2005) also reported a significant increase (66.1 vs. 62.4%) in the fertility rate when thawing is done in warm-water 35°C for 30 seconds compared to pocket-thaw.…”

Section: Discussionmentioning

confidence: 89%

Survey of Artificial Insemination Practices in Algeria

Souames¹

2015

Res. j. vet. pract.

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 89%

Survey of Artificial Insemination Practices in Algeria

Souames¹

2015

Res. j. vet. pract.

View full text Add to dashboard Cite

“…Notably, as Samples A and B differ in both their handling and their source animal, we will not seek to draw biological conclusions from any further observed distinctions between them, and place emphasis wholly on the comparative methodology presented in this work that is exemplified by the consideration of these two samples. After collection, the semen was first diluted in OptiXcell extender (IMV t Technologies, L'Aigle, France), and then frozen according to the standard procedures followed at Genex [36]. The thawing process is similar to our previous procedures for thawing frozen semen straws [37].…”

Section: Sample Preparationmentioning

confidence: 99%

Computer-assisted beat-pattern analysis and the flagellar waveforms of bovine spermatozoa

Walker

Phuyal

Ishimoto

et al. 2020

Preprint

View full text Add to dashboard Cite

Plagued by hurdles in information extraction, handling, and processing, computer-assisted sperm analysis (CASA) systems have typically neglected the complex flagellar waveforms of spermatozoa, despite their defining role in cell motility. Recent developments in imaging techniques and data processing have produced significantly-improved methods of waveform digitisation. Here, we utilise these improvements to demonstrate that near-complete flagellar capture is realisable on the scale of hundreds of cells, and, further, that meaningful statistical comparisons of flagellar waveforms may be readily performed with widely-available tools. Representing the advent of high-fidelity computer-assisted beat-pattern analysis (CABA), we show how such a statistical approach can distinguish between samples using complex flagellar beating patterns rather than crude summary statistics. Dimensionality-reduction techniques applied to entire samples also reveal qualitatively-distinct components of the beat, and a novel data-driven methodology for the generation of representative synthetic waveform data is proposed.

show abstract

“…The straws had been diluted in milk extender according to the standard procedures followed at Genex Cooperative, Inc., which are described by Kaproth et al (2005). Straws from at least three separate ejaculates from each bull were used.…”

Section: Sperm Processingmentioning

confidence: 99%

Cryopreservation increases coating of bull sperm by seminal plasma binder of sperm proteins BSP1, BSP3, and BSP5

Ardón

Suárez

2013

Reproduction

View full text Add to dashboard Cite

Artificial insemination with frozen semen allows affordable, worldwide dissemination of gametes with superior genetics. Nevertheless, sperm are damaged by the cryopreservation process. Elucidating the molecular effects of cryopreservation on sperm could suggest methods for improving fertility of frozen/thawed semen. This study was undertaken to examine the effect of cryopreservation on the coating of sperm by binder of sperm (BSP) proteins in seminal plasma. BSP proteins are secreted by the seminal vesicles and coat the surface of sperm by partially intercalating into the outer leaflet of the sperm plasma membrane. The BSP proteins are known to play roles in the formation of the oviductal sperm storage reservoir and in sperm capacitation. We investigated the effects of cryopreservation on the sperm BSP protein coat using Bovipure to separate live sperm from extended semen and then assaying the amounts of BSP proteins on sperm using quantitative western blotting with custom-made antibodies against unique sequences of each BSP protein. Greater amounts of all three BSP proteins (BSP1, BSP3, and BSP5) were detected on frozen/thawed sperm than on fresh sperm. Furthermore, the reduction of BSP3 from 15 to 13 kDa in mass, which occurs during incubation of sperm under mild capacitating conditions, was enhanced by cryopreservation. We concluded that freezing alters the BSP protein coating on sperm, which could account in part for reduced fertility of cryopreserved semen samples.

show abstract

Effect of semen thaw method on conception rate in four large commercial dairy heifer herds

Cited by 17 publications

References 12 publications

Survey of Artificial Insemination Practices in Algeria

Survey of Artificial Insemination Practices in Algeria

Computer-assisted beat-pattern analysis and the flagellar waveforms of bovine spermatozoa

Cryopreservation increases coating of bull sperm by seminal plasma binder of sperm proteins BSP1, BSP3, and BSP5

Contact Info

Product

Resources

About