Effect of Semen Diluents, Dilution Rates and Storage Periods on
Live and Abnormal Spermatozoa of Pearl Guinea Fowls

Hudson, G.H.; Omprakash, A.V.; Premavalli, K.

doi:10.3923/ajava.2016.411.416

Cited by 12 publications

(14 citation statements)

References 15 publications

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“…Although 24 h storage of chicken semen caused significant decrease in the number of live normal sperm, regardless of extender, it was significantly lower than in neat, undiluted semen samples, what may indicate a beneficial effect of extender additives tested in this experiment. Similar as in our experiment, many researchers also discussed that during poultry semen storage, the number of live properly formed sperm decreases and deformed or dead sperm increases, regardless of the type of diluent and the antioxidant added ( Barber et al., 2005 , Hudson et al., 2016 ). Although antioxidants do not prevent completely the adverse changes caused by storage, they significantly reduce their effects.…”

Section: Discussionsupporting

confidence: 85%

“…In case of poultry reproduction, artificial insemination that allows to decrease the number of males in the flock, certainly contributes to this solution. The semen intended for insemination must be of very good quality, which depends, among others, on genetic origin ( Siudzińska and Łukaszewicz, 2008a , Tabatabatei et al., 2009 , Kowalczyk and Łukaszewicz, 2012 , Ameen et al., 2014 ; Kotłowska et al., 2005), content of nutrients, especially antioxidants ( Dimitrov et al., 2007 , Jerysz and Łukaszewicz, 2013 ) that affect tissue activity during spermatogenesis ( Surai et al., 1998 , Barber et al., 2005 ), type of semen extenders selected for the species ( Marzoni et al., 2003 , Hudson et al., 2016 , Kowalczyk et al., 2017 ), time elapsing from semen collection, and storage conditions before insemination ( Siudzińska and Łukaszewicz, 2008b ).…”

Section: Introductionmentioning

confidence: 99%

“…The most desired and valuable is semen containing as many live, viable sperm with proper morphological structure as possible, because only such cells have the highest potential fertilizing ability. However, it is well known that freshly collected, neat avian semen stored in vitro very quickly loses its viability and motility ( Jafar et al., 2013 , Das et al., 2015 ) and as the consequence, the fertilizing ability ( Hudson et al., 2016 ). There is a growing interest in developing an efficient environment for storing semen in a liquid stage for prolonged time without significant decrease in its quality and fertilizing potency.…”

Section: Introductionmentioning

confidence: 99%

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Effect of semen extenders on viability of ISA Brown and Hubbard Flex roosters' sperm stored for 24 h

2020

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Artificial insemination is used in almost 95% of turkey reproductive flocks and is becoming more important in chickens, particularly broiler breeders, as well as in assisted reproduction of wild birds kept in breeding centers. Diluted semen is recommended for artificial insemination. Pooled semen samples collected twice a week by dorso-abdominal massage from 2 chicken lines: laying—ISA Brown ( ISA-B ) and meat type—Hubbard Flex ( H-F ) were divided into 5 parts: neat semen and diluted in 1:2 ratio with 4 extenders: basic EK; EK + 1 μg/mL organic selenium and 8 μg/mL vitamin E; EK + 10 mg/mL of royal jelly; and EK + 0.25 g/mL of lyophilized bovine colostrum. Diluted semen samples were evaluated after 15 min and then 24 h storage at 4°C. Sperm concentration, motility, motility parameters (with Sperm Class Analyzer), and morphology were evaluated in the neat semen, whereas in diluted and stored samples, the last 3 traits were determined. In case of both lines, dilution did not affect ( P > 0.05) the number of live normal cells (78.0–81.1% in ISA Brown and 73.8–68.7% in Hubbard Flex) in relation to neat semen; however, bovine colostrum addition increased ( P < 0.05) the percentage of bulb head sperm (5.7 vs. 10.0% and 12.1 vs. 17.6%, for ISA and Hubbard, respectively) and decreased sperm motility (67.4 vs. 92.9% and 67.3 vs. 98.5% for ISA and Hubbard). The 24 h storage of neat semen and semen diluted with colostrum caused ( P < 0.05) the unfavorable changes in all evaluated traits and both chicken lines, whereas semen dilution with remaining extenders decreased the percentage of live normal cells (by 18.8–23.4% ISA and by 20.9–25.5% Hubbard) but did not affect sperm motility (81.5–87.6% for ISA and 81.1–96.6% for Hubbard). Sperm motility and motility parameters depended both on the extender and chicken line.

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Section: Discussionsupporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of semen extenders on viability of ISA Brown and Hubbard Flex roosters' sperm stored for 24 h

2020

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“…In this study, decrease in motility, live cells, morphologically normal spermatozoa and an increase in dead spermatozoa, spermatozoa with damaged acrosomes and coiled tails were observed with increase in storage period. This corroborates the findings of Hudson et al (2016) who reported significant increase in percentage dead and abnormal spermatozoa with increasing storage time. However, semen extended with EYP+PBS had higher values than the EY+PBS group in this regard.…”

Section: Discussionsupporting

confidence: 92%

Evaluation of Egg Yolk Plasma as Replacement for Whole Egg Yolk in Chicken Semen Extender

Ola

Faleye

Adeyemi

et al. 2020

Tavukçuluk Araştırma Dergisi

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Poultry semen has high spermatozoa concentration and needs to be extended (diluted) for efficient artificial insemination; extenders containing egg yolk often have various limitations to their use. This study assessed chicken egg yolk plasma (EYP) as replacement for chicken whole egg yolk (EY) in semen extenders. The preservative potential under room and cold storage and its influence on fertility and hatchability in breeder chicken flock was also assessed. Ten broiler breeder cocks and one hundred hens were used for the study. Semen ejaculates from the cocks were pooled and divided into five portions. One portion each was extended with egg yolk plasma (EYP); phosphate buffer saline (PBS); egg yolk (EY) +PBS or EYP +PBS while the fifth unextended portion termed fresh undiluted semen (FUS) served as the control. The ratio of EYP and EY to PBS was 1:4. The semen samples were evaluated at 1, 2 and 3 hours after extension under room temperature and at 24, 48 and 72 hours after cold storage (4℃). The hens were inseminated with the freshly extended semen or with cold preserved semen, each for three weeks. Fertility and hatchability of the eggs were recorded on 18th and 21st day, respectively post setting. At room and cold storage temperatures, the quality parameters of the semen significantly reduced as the holding time increased. The EYP groups had the highest spermatozoa motility of 55.33% at 72 hours of cold storage. Freshly extended semen with EYP+ PBS had higher quality and fertilizing potentials which resulted in increased egg fertility (87.49%) and hatchability (84.95%). Cold preserved EYP + PBS semen resulted in significantly higher fertility (75.66%) and hatchability (90.90%). It was concluded that egg yolk plasma could conveniently replace whole egg yolk in chicken semen extender resulting in improved sperm viability and egg hatchability.

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“…Insufficient practical application of semen cryopreservation and artificial insemination of birds, which is partly due to the low efficiency of existing methods of freezing ejaculates, is a constraint on the development of the poultry industry (Dimitrov, Atanasov, Surai, Denev, 2007;Donoghue, Wishart, 2000;Douard, Hermier, Magistrini, Blesbois, 2003). In the process of freezing and thawing of ejaculates of birds a sharp decrease in physiological characteristics of spermatozoa is observed that is explained by a high level of damage of plasma membranes of sexual cells of birds (Fattah, Sharafi, Masoudi, Shahverdi, Najafi, 2017;Froman, 2003;Hudson, Omprakash, Premavalli, 2016). First of all, it indicates the imperfection of existing technological approaches and applied diluents for bird ejaculates (Iaffaldano, Meluzzi, 2003;Kotlowska, Dietrich, Wojtczak, Karol, Ciereszko, 2007;Kucera, Heidinger, 2018).…”

Section: Introductionmentioning

confidence: 99%

Modern Technology of Poultry Semen Cryopreservation

Tkachev¹,

Tkachevа²,

Gazzavi-Rogozina³

et al. 2020

Modern S&T Equipments and Problems in Agriculture

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This article presents results of comparison of efficiency of cryopreservation of sperm of a poultry on Belgorod and German technology and physiological features of sperm of males of birds of different kinds. Ejaculates from experimental birds were received 3 times a week by spin peritoneum massage. Efficiency of Belgorod technology of semen cryopreservation, which was modified by us for semen of birds, provided better physiological characteristics, separated ejaculates of poultry after thawing, than German technology. The mobility of rooster sperm after decontamination was 4.24 % higher than that of the German technology with an increase in membrane conservation by 8.6 %. Cryoresistance of quail semen was also higher at application of Belgorod technology by 3.24 % on mobility and by 3,26 % on membrane-stabilizing properties. The efficiency of guinea fowls semen cryopreservation according to Belgorod technology in lined granules was 4.44% higher than the German analogue in terms of sperm motility, 8.87% higher in terms of integrity of plasma membranes of germ cells. Physiological characteristics of thawed turkey semen, which was prepared according to the Belgorod technology, exceeded the German analogue by 7.04% in terms of mobility and by 2.65% in the integrity of the membranes of sperm cells.

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Effect of Semen Diluents, Dilution Rates and Storage Periods on Live and Abnormal Spermatozoa of Pearl Guinea Fowls

Cited by 12 publications

References 15 publications

Effect of semen extenders on viability of ISA Brown and Hubbard Flex roosters' sperm stored for 24 h

Effect of semen extenders on viability of ISA Brown and Hubbard Flex roosters' sperm stored for 24 h

Evaluation of Egg Yolk Plasma as Replacement for Whole Egg Yolk in Chicken Semen Extender

Modern Technology of Poultry Semen Cryopreservation

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