Effect of semen collection frequency on the semen characteristics of dromedary camels

Al-Bulushi, Samir; Manjunatha, B.M.; Bathgate, R.; Rickard, J.P.; Graaf, S.P. de

doi:10.1016/j.anireprosci.2018.08.022

Cited by 18 publications

(17 citation statements)

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“…The effect of semen collection frequency on the semen quality has already studied in farm animals; such as bucks (Oyeyemi et al, 2000), rams (Aguirre et al, 2007;Yotov et al, 2011), boars (Pruneda et al, 2005), bulls (Rashid et al, 2015) and camels (Al-Bulushi et al, 2018), these studies have limited to evaluate the effect of semen collection frequency on the semen characteristic. Our study has been planned to observe the effect of semen collection frequency on the success of the buck semen dilution.…”

Section: Discussionmentioning

confidence: 99%

The influence of semen collection frequency on deleterious the interaction between the enzymes of the bulbourethral gland and egg yolk during the dilution of buck semen

Dhaher

Aziz

2021

SVU-International Journal of Veterinary Sciences

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The study aimed to evaluate the influence of semen collection frequency at different protocols on deleterious the interaction between the enzymes of the bulbourethral gland and egg yolk during the dilution and of buck semen. Five bucks aged 2.5-3 years were included in this study. Four different protocols of semen collection frequency were applied; once a week, twice a week at an interval of two days, thrice a week at an interval of one day, and twice daily at an interval of one hour. Buck semen ejaculates were evaluated using computer assisted sperm analysis that includes the percentage of motile sperm (MS), percentage of progressive motile sperm (PMS), velocity curvilinear (VCL), velocity average path (VAP), velocity straight line (VSL), linearity (LIN), straightness (STR), wobble (WOB), amplitude of lateral head movement (ALH), and beat cross frequency (BCF). Results showed that sperm motility parameters had decreased significantly after dilution. Sperm motility parameters (VCL, VSL, VAP and ALH) of the second ejaculate were higher significantly (P<0.05) after dilution, when ejaculates collected twice and thrice a week and twice a day. Conversely, motility parameters of third ejaculates were less than those of first and second ejaculates when ejaculates collected thrice a week. In conclusion, there are effects of semen collection frequency on the ability of buck semen dilution, and the best results were when ejaculates had collected twice a day at an interval of one hour, accordingly we recommended this protocol for preparation of buck semen for artificial insemination.

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Section: Discussionmentioning

confidence: 99%

The influence of semen collection frequency on deleterious the interaction between the enzymes of the bulbourethral gland and egg yolk during the dilution of buck semen

Dhaher

Aziz

2021

SVU-International Journal of Veterinary Sciences

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“…Semen was collected at weekly intervals as described previously [21] using a bovine artificial vagina (AV; 30 cm length and 5 cm internal diameter). A smooth latex liner was mounted and fixed at both ends of the AV and a transparent graduated glass water-jacketed semen collection vessel was attached to the apex of the internal latex liner.…”

Section: Methodsmentioning

confidence: 99%

“…Liquification status was assessed by pipetting at 5 min intervals. The average liquefaction of semen occurred 17.5 ± 0.6 min after 1:1 dilution with extender [21]. After complete liquefaction, sperm concentration was determined using a Makler Counting Chamber (Sefi-Medical Instruments, Haifa, Israel) [22].…”

Section: Methodsmentioning

confidence: 99%

“…Motion characteristics of spermatozoa were evaluated using computer assisted sperm assessment (CASA; CEROS, Version12, Hamilton Thorne Biosciences, USA) as described previously [21]. The following CASA parameters were used; frame rate = 60 Hz; number of frames acquired = 45; minimum contrast = 55; minimum cell size (pixels) = 6; medium average path velocity cut-off = 30 μm/s; medium threshold straightness = 60%; slow average path velocity cut-off = 10 μm/s; slow straight line velocity cut-off = 5 μm/s; static average path velocity cut-off = 4 μm/s; static straight line velocity cut-off = 1 μm/s; non-motile head size (pixels) = 0.5 to 4.8; non-motile head intensity = 0.25 to 1.8; magnification = 1.87x; video frequency = 60; illumination intensity = 2300; temperature = 37°C.…”

Section: Methodsmentioning

confidence: 99%

“…Five randomly selected microscopic fields were scanned and approximately 500 spermatozoa counted. The total motility (TM), progressive motility (PM), average path velocity (VAP), progressive velocity (VSL) and track speed (VCL), lateral head amplitude (ALH), beat cross frequency (BCF), straightness (STR) and linearity (LIN) of spermatozoa were analysed with camel settings [21].…”

Section: Methodsmentioning

confidence: 99%

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Artificial insemination with fresh, liquid stored and frozen thawed semen in dromedary camels

et al. 2019

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This study was conducted to evaluate various factors affecting fertility following insemination of dromedary camels. In experiment 1, camels were either bred by natural mating (NM) or inseminated in the body of uterus with whole, split (50:50) or 1 mL of undiluted ejaculate. In experiment 2, camels were inseminated with fresh diluted semen either in the body of the uterus or tip of the uterine horn and at either the time of ovulation induction (0 h), 24 or 30 h later. In experiment 3, camels were inseminated at the tip of the uterine horn with different doses of fresh diluted semen (75, 150 or 300 x 106 motile spermatozoa) or with 150 x 106 motile spermatozoa diluted with different extenders (Green buffer, Optixcell or Triladyl). In experiment 4, camels were inseminated in the tip of the uterine horn with diluted (Triladyl or Optixcell) liquid-stored semen or diluted (Triladyl) frozen-thawed semen consisting of either 300 or 500 x 106 motile spermatozoa. The pregnancy rate in camels bred by NM was similar to camels inseminated with whole undiluted ejaculates whereas insemination with 1 mL undiluted ejaculate resulted in lower pregnancy compared to whole and split undiluted ejaculates (P < 0.05). Deposition of semen in the uterine body resulted in lower pregnancy rates compared to deposition in the tip of the horn (35.3% versus 72.2%, P < 0.05) but insemination at the time of ovulation induction and 24 h later resulted in higher pregnancy rate to camels inseminated at 30 h after induction (68.4 and 70.0% versus 23.5%; P < 0.05). Artificial insemination with 75 x 106 motile spermatozoa resulted in lower pregnancy rates compared to 150 and 300 x 106 motile spermatozoa doses (40.9% versus 65.2 and 70.0%, respectively) and pregnancy rate was not affected by extenders. Insemination of chilled motile spermatozoa stored in either Triladyl or Optixcell resulted in similar pregnancy rates, regardless of insemination dose, although an upward trend with increasing sperm number was apparent (Triladyl; 11.1% versus 21.1% and Optixcell; 5.9% versus 12.5%, for 300 x 106 and 500 x 106 groups, respectively; P > 0.05). No pregnancies were obtained with frozen thawed semen. In conclusion, this study demonstrated that the success of camel AI is highly dependent on sperm dose, location of semen deposition, timing of insemination and semen type. Further studies are required to determine the reason for the compromised fertility of preserved semen despite apparent high in vitro quality.

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Reproduction Management and Artificial Insemination in Dromedary Camel

Gherissi

Lamraoui

2021

Sustainable Agriculture Reviews

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Effect of semen collection frequency on the semen characteristics of dromedary camels

Cited by 18 publications

References 18 publications

The influence of semen collection frequency on deleterious the interaction between the enzymes of the bulbourethral gland and egg yolk during the dilution of buck semen

The influence of semen collection frequency on deleterious the interaction between the enzymes of the bulbourethral gland and egg yolk during the dilution of buck semen

Artificial insemination with fresh, liquid stored and frozen thawed semen in dromedary camels

Reproduction Management and Artificial Insemination in Dromedary Camel

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