Effect of Self- and Inter-Cultivar Grafting on Growth and Nutrient Content in Sweet Basil (Ocimum basilicum L.)

Abstract

Vegetable grafting has been applied to fruiting crops, yet only to a limited extent in leafy greens and herbs which may also benefit from grafting. In this study, we examined the effect of reciprocal grafting two sweet basil (Ocimum basilicum L.) cultivars of differing vigor on plant growth and leaf mineral nutrient concentration to test whether differences in growth exist due to grafting and whether such differences are due to altered plant nutrient status in two trials. Two cultivars ‘Nufar’ (NU), a high vigor cultivar, and ‘Dolce Fresca’ (DF), a cultivar developed for compact growth, were selected. Four grafted treatments (scion/rootstock) were created by self-grafting (NU/NU and DF/DF) and reciprocal inter-cultivar grafting (DF/NU and NU/DF). Un-grafted plants (ug-NU and ug-DF) served as controls. Following grafting, plants were grown for 26 or 21 days in a greenhouse. DF rootstocks decreased NU shoot dry mass (19–29%) and stem length (12%) compared to ug-NU in both trials, while dry root mass was reduced (28%) in the second trial. In contrast, NU rootstocks did not affect DF growth in the first trial but significantly decreased dry shoot (18%) and root (31%) mass, compared to ug-DF in the second. Concentration of most inorganic nutrients examined was affected by both rootstock and scion genotype. For NU scions, DF rootstocks resulted in significantly higher (5–29%) levels of nitrogen, phosphorus, potassium, calcium, magnesium, sulfur, copper, and zinc in leaf tissue than ug-NU. For DF scions, NU rootstocks resulted in significantly higher (7–9%) levels of potassium and phosphorus but significantly lower (11–23%) levels of magnesium, sulfur, boron, copper, and zinc when compared to ug-DF. Results of this study show that inter-cultivar grafting sweet basil using a more vigorous cultivar as a rootstock did not enhance the growth of a less vigorous scion and reduced concentrations of certain nutrients. However, grafting a vigorous scion to a less vigorous rootstock reduced shoot growth but generally increased nutrient concentrations. This suggests that changes in growth in grafted basil are not due to altered nutrient status. Further research is needed to determine specific physiological processes influencing grafted basil growth.

Grafting in Capsicum peppers as a strategy to mitigate the effects of climate change on yield and quality factors

“…By contrast, as mentioned before, other genotypes including accessions from other species like C. chinense U26484 and C. baccatum U27610, showed a similar performance as rootstocks than 'Herminio' itself. Although other authors have not reported significant decreases in the yield of self-grafted varieties (Hollick and Kubota, 2022), our results suggest that, in the case of some varieties of peppers, self-grafting may provoke injuries in the rootstock-scion connection, while paradoxically other materials used as rootstocks may show better compatibility by providing a higher efficiency in restoring the phloem and xylem and then promoting a better mobilization of water and nutrients (Ropokis et al, 2019).…”

Physiological, Metabolic, and Transcriptomic Analyses Reveal the Effect of Sweet Corn Plants Treated with a New Biostimulant on the Growth Enhancement, Focusing on Enhancing Gibberellin Synthesis and Signal Transduction

“…The total antioxidant activity was measured according to Ertani et al The content of the hormones was measured according to Yan et al The N, P, and K contents were measured according to Kubota and Hollick …”

“…The total antioxidant activity was measured according to Ertani et al 10 The content of the hormones was measured according to Yan et al 11 The N, P, and K contents were measured according to Kubota and Hollick. 12 RNA Isolation and Transcriptome Sequencing. Total RNA was extracted from tissues using TRIzol reagent, and genomic DNA was removed using DNase I (Takara) according to the manufacturer's (Invitrogen) instructions.…”