Effect of selenium supplementation on the activities of glutathione metabolizing enzymes in human hepatoma Hep G2 cell line

Helmy, Mai M.; S, Ismail; Fayed, Hassan; El-Bassiouni, E. A.

doi:10.1016/s0300-483x(99)00190-0

Cited by 40 publications

(24 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Transferrin is also an essential protein in culture medium where the main action is to transfer iron into the cells (Bjare, 1992). Selenium is an essential trace element and acts in particular in selenoproteins which protect cells against oxidative stress (Helmy et al, 2000).…”

Section: Basal Mediummentioning

confidence: 99%

Optimization of chemically defined cell culture media – Replacing fetal bovine serum in mammalian in vitro methods

Valk

Brunner

Smet³

et al. 2010

Toxicology in Vitro

485

379

View full text Add to dashboard Cite

Section: Basal Mediummentioning

confidence: 99%

Optimization of chemically defined cell culture media – Replacing fetal bovine serum in mammalian in vitro methods

Valk

Brunner

Smet³

et al. 2010

Toxicology in Vitro

485

379

View full text Add to dashboard Cite

“…43 Enzyme activity of GPx is also upregulated by selenium in human cell lines. 44,45 There are only a few published studies on the in vitro effects of selenium on prostate cancer cell lines and most of them described an inhibitory effect of selenium on prostate cancer cells as an acute short-term effect. 46 -49 Oberlay and colleagues recently published their results on the effects of chronic treatment with selenium on prostate cancer cells.…”

Section: Effect Of Genistein On Antioxidant Enzymes Activities In Lncmentioning

confidence: 99%

Genistein, a soy isoflavone, induces glutathione peroxidase in the human prostate cancer cell lines LNCaP and PC‐3

Suzuki

Koike

Matsui

et al. 2002

Intl Journal of Cancer

170

View full text Add to dashboard Cite

Genistein is a major component of soybean isoflavone and has multiple functions resulting in antitumor effects. Prostate cancer is 1 of the targets for the preventive role of genistein. We examined the effect of genistein on human prostate cancer (LNCaP and PC-3) cells. Proliferation of both cell lines was inhibited by genistein treatment in a dose-dependent manner. To obtain the gene expression profile of genistein in LNCaP cells, we performed cDNA microarray analysis. The expression of many genes, including apoptosis inhibitor (survivin), DNA topoisomerase II, cell division cycle 6 (CDC6) and mitogen-activated protein kinase 6 (MAPK 6), was downregulated. Expression levels were increased more than 2-fold in only 4 genes. The glutathione peroxidase (GPx)-1 gene expression level was the most upregulated. Quantitative real-time polymerase chain reaction revealed significant elevation of transcript levels of GPx-1 in both LNCaP and PC-3 cells. Upregulation of gene expression levels accompanied elevation of GPx enzyme activities. In contrast, no significant changes were observed in the gene expression levels and enzyme activities of the other antioxidant enzymes, superoxide dismutase and catalase. GPx activation might be one of the important characteristics of the effects of genistein on prostate cancer cells.

show abstract

“…25 Transferrin is an essential protein that is known to transport iron into cells, where iron plays a role in the hydroxylation of proline during collagen synthesis, 26,27 and selenium is an essential trace element that boosts the activity of glutathione peroxidases in shielding cells from oxidative stress. 28 ITS-supplemented media have been shown to viably support and promote matrix deposition within chondrocyte-seeded agarose constructs. 29,30 The effects of relative concentrations of ITS on cell proliferation have been investigated in 2D monolayer culture of rat neuroblastoma cells 31 and HeLa cells, but the specific effects of ITS concentrations on the functional properties of engineered cartilage have not been characterized.…”

Section: Introductionmentioning

confidence: 99%

Insulin, Ascorbate, and Glucose Have a Much Greater Influence Than Transferrin and Selenous Acid on theIn VitroGrowth of Engineered Cartilage in Chondrogenic Media

Cigáň

Nims

Albro

et al. 2013

Tissue Engineering Part A

View full text Add to dashboard Cite

The primary goal of this study was to characterize the response of chondrocyte-seeded agarose constructs to varying concentrations of several key nutrients in a chondrogenic medium, within the overall context of optimizing the key nutrients and the placement of nutrient channels for successful growth of cartilage tissue constructs large enough to be clinically relevant in the treatment of osteoarthritis (OA). To this end, chondrocyte-agarose constructs (ø4 · 2.34 mm, 30 · 10 6 cells/mL) were subjected to varying supplementation levels of insulin (0· to 30· relative to standard supplementation), transferrin (0· to 30·), selenous acid (0· to 10·), ascorbate (0· to 30· ), and glucose (0· to 3·). The quality of resulting engineered tissue constructs was evaluated by their compressive modulus (E -Y ), tensile modulus (E +Y ), hydraulic permeability (k), and content of sulfated glycosaminoglycans (sGAG) and collagen (COL); DNA content was also quantified. Three control groups from two separate castings of constructs (1· concentrations of all medium constituents) were used. After 42 days of culture, values in each of these controls were, respectively, E -Y = 518 -78, 401 -113, 236 -67 kPa; E +Y = 1420 -430, 1140 -490, 1240 -280 kPa; k = 2.3 -0.8 · 10 -3 , 5.4 -7.0 · 10 -3 , 3.3 -1.3 · 10 -3 mm 4 /N$s; sGAG = 7.8 -0.3, 6.3 -0.4, 4.1 -0.5%/ww; COL = 1.3 -0.2, 1.1 -0.3, 1.4 -0.4%/ww; and DNA = 11.5 -2.2, 12.1 -0.6, 5.2 -2.8 mg/disk. The presence of insulin and ascorbate was essential, but their concentrations may drop as low as 0.3· without detrimental effects on any of the measured properties; excessive supplementation of ascorbate (up to 30·) was detrimental to E -Y , and 30· insulin was detrimental to both E +Y and E -Y . The presence of glucose was similarly essential, and matrix elaboration was significantly dependent on its concentration ( p < 10 -6

show abstract

Effect of selenium supplementation on the activities of glutathione metabolizing enzymes in human hepatoma Hep G2 cell line

Cited by 40 publications

References 15 publications

Optimization of chemically defined cell culture media – Replacing fetal bovine serum in mammalian in vitro methods

Optimization of chemically defined cell culture media – Replacing fetal bovine serum in mammalian in vitro methods

Genistein, a soy isoflavone, induces glutathione peroxidase in the human prostate cancer cell lines LNCaP and PC‐3

Insulin, Ascorbate, and Glucose Have a Much Greater Influence Than Transferrin and Selenous Acid on theIn VitroGrowth of Engineered Cartilage in Chondrogenic Media

Contact Info

Product

Resources

About