Effect of selenium on characteristics of rape chloroplasts modified by cadmium

Filek, Maria; Gzyl-Malcher, Barbara; Zembala, Maria; Bednarska, E; Laggner, Peter; Kriechbaum, Manfred

doi:10.1016/j.jplph.2009.07.003

Cited by 99 publications

(35 citation statements)

References 39 publications

(46 reference statements)

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“…The hydrogen peroxide content showed no effect of Se at low concentration (1 ppm) and also remained low at 2 ppm, which is in line with the effects of Se application (2 μM) on rapeseed (Brassica napus) seedlings [48]. At higher Se levels (4 and 6 ppm), increase in hydrogen peroxide occurred indicating evocation of oxidative stress.…”

Section: Se Effectssupporting

confidence: 78%

Exogenous Proline Application Reduces Phytotoxic Effects of Selenium by Minimising Oxidative stress and Improves Growth in Bean (Phaseolus vulgaris L.) Seedlings

Aggarwal

Sharma

Kaur

et al. 2010

Biol Trace Elem Res

102

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Bean (Phaseolus vulgaris L.) seedlings were subjected to varying selenium levels (1, 2, 4, and 6 ppm) in a hydroponic culture. The germination reached 100% in 48 h in all Se levels except 6 ppm, where it took 72 h. The root and shoot growth was stimulated at 1 and 2 ppm Se levels that was commensurate with increase in chlorophyll content, leaf water content, and cellular respiration. At 4 and 6 ppm Se levels, the growth was inhibited appreciably, which was associated with increase in stress injury measured as damage to membranes and decrease in cellular respiration, chlorophyll, and leaf water content. The oxidative injury as elevation of lipid peroxidation was larger compared to hydrogen peroxide accompanied by reduced levels of enzymatic (superoxide dismutase, catalase, ascorbate peroxidase, and glutathione reductase) and non-enzymatic (ascorbic acid and glutathione) antioxidants. Proline content was significantly higher at 1 and 2 ppm Se but diminished considerably at 4 and 6 ppm levels concomitant with the reduced growth. Exogenous application of proline (50 µM) resulted in substantiation of its endogenous levels that antagonised the toxic effects of Se by improving the growth of seedlings. The stress injury was reduced significantly with simultaneous increase in enzymatic and non-enzymatic antioxidants. Especially the components of ascorbate-glutathione cycle showed larger stimulation with proline application. The role of proline in mitigating the toxic effects of Se is discussed.

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Section: Se Effectssupporting

confidence: 78%

Exogenous Proline Application Reduces Phytotoxic Effects of Selenium by Minimising Oxidative stress and Improves Growth in Bean (Phaseolus vulgaris L.) Seedlings

Aggarwal

Sharma

Kaur

et al. 2010

Biol Trace Elem Res

102

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“…However, at high doses, Se acts as pro-oxidant and causes oxidative stress in plants, for e.g., in Pb stressed roots of Vicia faba 1.5 μM Se decreased ROS but 6 μM increased ROS accumulation and decreased cell viability (Mroczek-Zdyrska and Wójcik, 2012). Filek et al (2010) reported positive effect of 2 μM Se in maintaining the structure and fluidity of chloroplast membrane in Cd-stressed rape seedlings. Similar effect of low dose of Se on plastid membranes was also reported in Cd-stressed wheat seedlings (Filek et al, 2009), which could be attributed to reactivation of membrane enzymes or transportation of metabolites in chloroplast upon Se application.…”

Section: Beneficial Effects Of Selenium In Plantsmentioning

confidence: 99%

An Overview of Selenium Uptake, Metabolism, and Toxicity in Plants

2017

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Selenium (Se) is an essential micronutrient for humans and animals, but lead to toxicity when taken in excessive amounts. Plants are the main source of dietary Se, but essentiality of Se for plants is still controversial. However, Se at low doses protects the plants from variety of abiotic stresses such as cold, drought, desiccation, and metal stress. In animals, Se acts as an antioxidant and helps in reproduction, immune responses, thyroid hormone metabolism. Selenium is chemically similar to sulfur, hence taken up inside the plants via sulfur transporters present inside root plasma membrane, metabolized via sulfur assimilatory pathway, and volatilized into atmosphere. Selenium induced oxidative stress, distorted protein structure and function, are the main causes of Se toxicity in plants at high doses. Plants can play vital role in overcoming Se deficiency and Se toxicity in different regions of the world, hence, detailed mechanism of Se metabolism inside the plants is necessary for designing effective Se phytoremediation and biofortification strategies.

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“…At the molecular level, it was proved that the short-term exposure of plants to relatively large ozone concentrations (0.15–0.6 ppm) results in a significant reduction in the amount of galactolipids in the chloroplasts membranes and in a decrease of the fatty acid chains unsaturation (Harwood 1998). It was found (Filek et al 2010a) that the aspect ratio of MGDG to DGDG in chloroplast envelopes of rape exposed to oxidative stress was changed. ROS excess generated under oxidative stress may cause lipid peroxidation, the measure of which may be an increase in the concentration of malondialdehyde (MDA) (Moore and Roberts 1998).…”

Section: Introductionmentioning

confidence: 99%

Physicochemical Aspects of Reaction of Ozone with Galactolipid and Galactolipid–Tocopherol Layers

2014

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The impact of reaction of galactolipids with ozone on the physicochemical properties of their monolayers was examined. In Megli and Russo (Biochim Biophys Acta, 1778:143–152, 2008), Cwiklik and Jungwirth (Chem Phys Lett, 486:99–103, 2010), Jurkiewicz et al. (Biochim Biophys Acta, 1818:2388–2402, 2012), Khabiri et al. (Chem Phys Lett, 519:93–99, 2012), and Conte et al. (Biochim Biophys Acta, 1828:510–517, 2013), the properties of layers formed from model mixtures composed of chosen lipids and selected oxidation products were studied, whereas in this work, question was raised as to how the oxidation reactions taking place in situ affect the physical properties of the galactolipid layers. So, set experiment should take into account the effect of all reaction products. The mechanical characteristics of monolayers of monogalactosyldiacyl-glycerol (MGDG) and digalactosyldiacylglycerol (DGDG) were determined by Langmuir trough technique, and the electrical properties of liposomes formed from these lipids by measuring their electrophoretic mobility. Considerable loss of galactolipid molecules forming monolayers was found at ozone concentrations (in aqueous medium) higher than 0.1 ppm with a stronger effect measured for MGDG. That goes along with the greater amounts of MDA found in the extracts of oxidized MGDG films compared with DGDG. Based on this, it was concluded that an additional galactose group present in DGDG molecules acts protectively under oxidative conditions. The surface tension of the solutions (of small volume) contacting the oxidized galactolipids films was significantly reduced, indicating the presence of soluble in polar media, surface active reaction products. The presence of α-tocopherol in mixtures with tested galactolipids at a molar ratio of lipid to tocopherol equal to 1.7:1 caused some inhibition of lipid oxidation, reducing the decrease of amount of lipid particles forming the monolayer. Here, also protective effect of α-tocopherol was greater for the MGDG compared to DGDG.

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Effect of selenium on characteristics of rape chloroplasts modified by cadmium

Cited by 99 publications

References 39 publications

Exogenous Proline Application Reduces Phytotoxic Effects of Selenium by Minimising Oxidative stress and Improves Growth in Bean (Phaseolus vulgaris L.) Seedlings

Exogenous Proline Application Reduces Phytotoxic Effects of Selenium by Minimising Oxidative stress and Improves Growth in Bean (Phaseolus vulgaris L.) Seedlings

An Overview of Selenium Uptake, Metabolism, and Toxicity in Plants

Physicochemical Aspects of Reaction of Ozone with Galactolipid and Galactolipid–Tocopherol Layers

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