Effect of selenium nanoparticles on biological and morphofunctional parameters of barley seeds (Hordéum vulgáre L.)

Abstract

The purpose of this work was to study the effect of selenium nanoparticles (Se NPs) on the biological and morphofunctional parameters of barley seeds (Hordéum vulgáre L.) We used seeds of Hordéum vulgáre L. with reduced morphofunctional characteristics. For the experiment, Se NPs were synthesized and stabilized with didecyldimethylammonium chloride. It was found that Se NPs have a spherical shape and a diameter of about 50 nm. According to dynamic light scattering data, the average hydrodynamic radius of the particles was 28 ± 8 nm. It is observed that the nanoparticles have a positive ζ-potential (+ 27.3 mV). For the experiment, we treated Hordéum vulgáre L. seeds with Se NPs (1, 5, 10 and 20 mg/L). The experiment showed that treatment of Hordéum vulgáre L. seeds with Se NPs has the best effect on the length of roots and sprout at concentration of 5 mg/L and on the number and thickness of roots at 10 mg/L. Germinability and germination energy of Hordéum vulgáre L. seeds were higher in group treated with 5 mg/L Se NPs. Analysis of macrophotographs of samples, histological sections of roots and 3D visualization of seeds by microcomputing tomography confirmed the best effect at 5 mg/L Se NPs. Moreover, no local destructions were detected at concentrations > 5 mg/L, which is most likely due to the inhibition of regulatory and catalytic processes in the germinating seeds. the treatment of Hordéum vulgáre L. seeds with > 5 mg/L Se NPs caused significant stress, coupled with intensive formation of reactive oxygen species, leading to a reorientation of root system growth towards thickening. Based on the results obtained, it was concluded that Se NPs at concentrations > 5 mg/L had a toxic effect. The treatment of barley seeds with 5% Se NPs showed maximum efficiency in the experiment, which allows us to further consider Se NPs as a stimulator for the growth and development of crop seeds under stress and reduced morphofunctional characteristics.

Effect of MnxOy Nanoparticles Stabilized with Methionine on Germination of Barley Seeds (Hordeum vulgare L.)

“…Another important area of application of nanomaterials, in particular, manganese nanoparticles and their oxides, is agriculture [31][32][33]. A large number of research papers are devoted to the study of the influence of nanoscale oxide forms on the morphofunctional characteristics of agricultural plants [34][35][36][37][38][39][40][41][42][43][44][45][46][47][48]. For example, the work of Margenot et al [49] shows that the addition of copper oxide (CuO) nanoparticles increases the diameter of the roots of lettuce seedlings by 52% and the diameter of carrot seedlings by 26%.…”

Impact of seed priming with Selenium nanoparticles on germination and seedlings growth of tomato

“…Moreover, in heat-stressed tomato plants, the concentrations of Se NPs with the best results on growth parameters were found to be 5 and 10 ppm, while 20 ppm showed signs of phytotoxicity 30 . In Barley ( Hordeum vulgare ) nanoprimed seeds with Se NPs, the best treatment for the germination quality in Petri dishes was also 5 ppm, while 10 ppm resulted in the highest number and thickness of roots 46 . In accordance with these studies, we found similar concentrations of Se NPs to have the best results in our nanopriming experiment for tomato seeds.…”

The Clothes Matter—Exploiting Agronomical Functions of Trichogenic Selenium Nanoparticles Sharing Activities with Biological Systems Wherein (Were) Formed

“…The application of Trichoderma strains on seeds promotes germination and seedling development [66,174,175]. The chemically synthetized SeNPs, stabilized with didecyldimethylammonium chloride, improved the germinability and germination of barley seeds (Hordéum vulgáre L.) with impaired morphofunctional characteristics due to 10 years of storage [176]. The trichogenic SeNPs should have a better effect as biostimulants of seed germination due to the combined effect of SeNPs and the bio-corona consisting of proteins and metabolites from biostimulant Trichoderma strains.…”