Effect of selected sampling media, flow rate, and time on the sampling efficiency of a liquid impinger packed with glass beads for the collection of airborne viruses

Chen, Yen‐Chi; Wang, I-Jen; Cheng, Chih-Ching; Wu, Yu-Chiao; Bai, Chun-Hsuan; Yu, Kuo Pin

doi:10.1007/s10453-020-09683-3

Cited by 8 publications

(7 citation statements)

References 37 publications

(36 reference statements)

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“…Unfortunately, we were not able to determine the viability of the virus for this study. Various factors influence the recovery of viable viruses, including collection methods, flow rates, sampling times, and sampling media [51,52]. At the time we designed the study, the viral resuspension rates were unknown, but expected to be low.…”

Section: Discussionmentioning

confidence: 99%

Resuspension of Seeded Particles Containing Live Influenza A Virus in a Full-Scale Laboratory

et al. 2023

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Many respiratory viruses, including influenza and SARS-CoV-2, are transmitted via the emission and inhalation of infectious respiratory aerosols in indoor environments. Resuspended particles from indoor surfaces and clothing can be a major source of airborne microbiological contaminants in indoor environments; however, it is unknown whether resuspended viruses contribute substantially to disease transmission. In this study, we investigated the resuspension via human walking activity of influenza A virus H3N2 laboratory strain, which was generated through a nebulizer into a sealed, unventilated biosafety level 2 (BSL-2) laboratory. The mean airborne viral concentrations following the resuspension events (3.7×103 viral RNA copies m−3) were two orders of magnitude lower than those following direct emission via the nebulizer (1.1×105 viral RNA copies m−3). The calculated resuspension emission factor (normalized ratio of the airborne mass to mass available for resuspension on the surface) of 10−3 was similar to reported values for 1–2 μm particles. Thus, depending on the infectious dose and viability of the virus, resuspension of settled respiratory viruses could lead to transmission, but the risk appears to be much lower than for direct respiratory emissions. To our knowledge, this is the first full-scale experimental study designed to quantify virus resuspension.

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Section: Discussionmentioning

confidence: 99%

Resuspension of Seeded Particles Containing Live Influenza A Virus in a Full-Scale Laboratory

et al. 2023

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“…As the key influencing factor of sampling aerosols, sampling flow rate has a significant effect on the total volume of sampling aerosol, sampling efficiency and biological sampling stress 52 , 53 . In our study, improvement of the total particle sampling efficiency of the AGI-30 and the BioSampler presented by the evaluation of AtoA sampling efficiency is achieved by increasing the sampling flow rate, but at the cost of increasing deposition on the inner surface of aerosol sampler as described in evaluation of AtoH sampling efficiency.…”

Section: Discussionmentioning

confidence: 99%

An experimental method for efficiently evaluating the size-resolved sampling efficiency of liquid-absorption aerosol samplers

Guo

Zheng

Qin

et al. 2022

Sci Rep

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Aerosol samplers are critical tools for studying indoor and outdoor aerosols. Development and evaluation of samplers is often labor-intensive and time-consuming due to the need to use monodisperse aerosols spanning a range of sizes. This study develops a rapid experimental methodology using polydisperse solid aerosols to evaluate size-resolved aerosol-to-aerosol (AtoA) and aerosol-to-hydrosol (AtoH) sampling efficiencies. Arizona Test Dust (diameter 0.5–20 µm) was generated and dispersed into an aerosol test chamber and two candidate samplers were tested. For the AtoA test, aerosols upstream and downstream of a sampler were measured using an online aerodynamic particle sizer. For the AtoH test, aerosols collected in sampling medium were mixed with a reference sample and then measured by the laser diffraction method. The experimental methodology were validated as an impressive time-saving procedure, with reasonable spatial uniformity and time stability of aerosols in the test chamber and an acceptable accuracy of absolute mass quantification of collected particles. Evaluation results showed that the AGI-30 and the BioSampler sampler had similar size-resolved sampling efficiencies and that efficiencies decreased with decreasing sampling flow rate. The combined evaluation of AtoA and AtoH efficiency provided more comprehensive performance indicators than either test alone. The experimental methodology presented here can facilitate the design and choice of aerosol sampler.

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“…The original liquid impinger design was proposed as early as 1947, which led to the development of the classic all-glass impinger (AGI), which operated at 12.5 L/min and collected particles into 20 mL of liquid [80]. The use of liquid media overcame overloading and enumeration problems [81], but the liquid media would dry out due to evaporation [82].…”

Section: Methods Of Samplingmentioning

confidence: 99%

Navigating the Aerosolized Frontier: A Comprehensive Review of Bioaerosol Research Post-COVID-19

Zhang,

Dai,

Gebrezgiabhier

et al. 2024

Atmosphere

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In the wake of the COVID-19 pandemic, the scientific community has been galvanized to unravel the enigmatic role of bioaerosols in the transmission of infectious agents. This literature review, anchored in the extensive Web of Science Core Collection database covering the period from 1990 to 2023, utilizes a bibliometric approach to chart the dynamic landscape of bioaerosol research. It meticulously documents the paradigm shifts and burgeoning areas of inquiry that have emerged in the aftermath of the pandemic. This review meticulously maps out the sources and detection strategies of pathogens in a variety of ecosystems. It clearly shows that impaction and filtration sampling methods, followed by colony counting and PCR-based detection techniques, were predominantly used in the scientific works within the previous three decades. It synthesizes the progress and limitations inherent in a range of models for predicting aerosol-mediated pathogen spread and provides a comparative analysis of eDNA technology and traditional analytical techniques for bioaerosols. The accuracy of these detection methods and forecasting models is paramount for the early recognition of transmission risks, which, in turn, paves the way for prompt and effective disease mitigation strategies. By providing a thorough analysis of the historical progression and current state of bioaerosol research, this review illuminates the path ahead, identifying the critical research needs that will drive the field’s advancement in the years to come.

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Effect of selected sampling media, flow rate, and time on the sampling efficiency of a liquid impinger packed with glass beads for the collection of airborne viruses

Cited by 8 publications

References 37 publications

Resuspension of Seeded Particles Containing Live Influenza A Virus in a Full-Scale Laboratory

Resuspension of Seeded Particles Containing Live Influenza A Virus in a Full-Scale Laboratory

An experimental method for efficiently evaluating the size-resolved sampling efficiency of liquid-absorption aerosol samplers

Navigating the Aerosolized Frontier: A Comprehensive Review of Bioaerosol Research Post-COVID-19

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