Effect of selected compounds on the activity of glutamate dehydrogenase from triticale roots

Kwinta, Joanna; Bartoszewicz, K.; Bielawski, W

doi:10.1007/s11738-002-0052-2

Cited by 2 publications

(1 citation statement)

References 22 publications

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“…The NAD + -dependent deamination and NADH-dependent amination activities of GDH were assayed by determining the concentration change of the coenzyme NADH in the reaction solution (Kumar et al, 2002;Kwinta et al, 2002;Restivo, 2004;Skopelitis et al, 2007) with modifications. Because GDH uses NAD + /NADH preferentially over NADP + /NADPH (Kwinta and Bielawski, 1998), only NAD + /NADH was used as a coenzyme for GDH in the present study.…”

Section: Methodsmentioning

confidence: 99%

Enzymatic Metabolism of Nitrogen in Leaves and Roots of Creeping Bentgrass under Nitrogen Deficiency Conditions

Jiang¹,

Xu²,

Huang³

2011

J. Amer. Soc. Hort. Sci.

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Low nitrogen (N) rates are recommended for creeping bentgrass (Agrostis stolonifera) putting greens to prevent excessive shoot growth and potential nitrate leaching, but low N rates could lead to N deficiency, which induces leaf senescence. This study was conducted to examine the effects of N deficiency on two enzymes involved in organic N metabolism as well as amino acid (AA) and soluble protein (SP) contents in both young and old leaves and roots of creeping bentgrass. Creeping bentgrass plants (cv. Penncross) were grown in a nutrient solution containing either 6 mm nitrate (+N plants) or zero N (−N plants), and each of the two treatments had four replicate pots. Young leaves on upper portions of the stolons and old leaves on lower portions of the stolons were separated and sampled at 14, 21, and 28 days of treatment, and roots were sampled at 28 days. Nitrogen deficiency increased glutamine synthetase (GS) transferase activity in all three tissues and at all three dates and GS biosynthetic activity in young leaves at all three dates. Prolonged N deficiency at 21 and 28 days increased glutamate dehydrogenase (GDH) deamination and amination activities in old leaves. In the roots, N deficiency at 28 days increased GS transferase activity but decreased GDH deamination activity. The N deficiency decreased AA content in all three tissues and at all three dates and SP content in young leaves at all three dates and in old leaves at 21 and 28 days. Decreasing organic N reserves in AA and SP and increasing GS and GDH activities in senescing leaves may be adaptive responses to N deficiency.

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Section: Methodsmentioning

confidence: 99%

Enzymatic Metabolism of Nitrogen in Leaves and Roots of Creeping Bentgrass under Nitrogen Deficiency Conditions

Jiang¹,

Xu²,

Huang³

2011

J. Amer. Soc. Hort. Sci.

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Quantitative Determination of Glycine in Aqueous Solution Using Glutamate Dehydrogenase-Immobilized Glyoxal Agarose Beads

Keskin

2013

Appl Biochem Biotechnol

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In this study, an enzymatic procedure for the determination of glycine (Gly) was developed by using a column containing immobilized glutamate dehydrogenase (GDH) on glyoxal agarose beads. Ammonia is produced from the enzymatic reactions between Gly and GDH with NAD(+) in phosphate buffer medium. The indophenol blue method was used for ammonia detection based on the spectrophotometric measurements of blue-colored product absorbing at 640 nm. The calibration graph is linear in the range of 0.1-10 mM of Gly concentrations. The effect of pH, temperature, and time interval was studied to find column stability, and also the interference effects of other amino acids was investigated. The interaction between GDH and glyoxal agarose beads was analyzed by Fourier transform infrared (FTIR) spectroscopy. The morphology of the immobilized and non-immobilized agarose beads were characterized by atomic force microscopy (AFM).

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Effect of selected compounds on the activity of glutamate dehydrogenase from triticale roots

Cited by 2 publications

References 22 publications

Enzymatic Metabolism of Nitrogen in Leaves and Roots of Creeping Bentgrass under Nitrogen Deficiency Conditions

Enzymatic Metabolism of Nitrogen in Leaves and Roots of Creeping Bentgrass under Nitrogen Deficiency Conditions

Quantitative Determination of Glycine in Aqueous Solution Using Glutamate Dehydrogenase-Immobilized Glyoxal Agarose Beads

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