Effect of selected catechins on doxorubicin antiproliferative efficacy and hepatotoxicity in vitro

Rudolfová, Petra; Hanušová, Veronika; Skálová, Lenka; Bártíková, Hana; Matoušková, Petra; Boušová, Iva

doi:10.2478/acph-2014-0018

Cited by 14 publications

(9 citation statements)

References 28 publications

(23 reference statements)

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“…The effects of AC on rat ileum and proximal colon viability and functionality were thus assessed, and results highlighted the safe profile of AC, indicating a selective activity against cancer tissues. This sparing activity was already reported for EGCG and EGC in many cancer cells lines [49][50][51][52][53], and although the precise mechanism is still debated, the lower intracellular copper content of non-malignant cells might constitute the key difference [54]. Finally, AC is endowed with antibacterial activity against Gram-negative (Escherichia coli, klebsiella pneumoniae, Proteus mirabilis and Pseudomonas aeruginosa) and Gram-positive bacteria (Staphylococcus aureus and Streptococcus pneumonia) [11,55]; at the same time it is ineffective toward Lactobacilli and Bifidobacteria, the most represented intestinal species.…”

Section: Discussionsupporting

confidence: 78%

Apoptotic-Induced Effects of Acacia Catechu Willd. Extract in Human Colon Cancer Cells

Chiaino

Micucci

Budriesi

et al. 2020

IJMS

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The research for innovative treatments against colon adenocarcinomas is still a great challenge. Acacia catechu Willd. heartwood extract (AC) has health-promoting qualities, especially at the gastrointestinal level. This study characterized AC for its catechins content and investigated the apoptosis-enhancing effect in human colorectal adenocarcinoma HT-29 cells, along with its ability to spare healthy tissue. MTT assay was used to describe the time course, concentration dependence and reversibility of AC-mediated cytotoxicity. Cell cycle analysis and AV-PI and DAPI-staining were performed to evaluate apoptosis, together with ROS formation, mitochondrial membrane potential (MMP) changes and caspase activities. Rat ileum and colon rings were tested for their viability and functionality to explore AC effects on healthy tissue. Quantitative analysis highlighted that AC was rich in (±)-catechin (31.5 ± 0.82 mg/g) and (−)-epicatechin (12.5 ± 0.42 mg/g). AC irreversibly decreased cell viability in a concentration-dependent, but not time-dependent fashion. Cytotoxicity was accompanied by increases in apoptotic cells and ROS, a reduction in MMP and increases in caspase-9 and 3 activities. AC did not affect rat ileum and colon rings' viability and functionality, suggesting a safe profile toward healthy tissue. The present findings outline the potential of AC for colon cancer treatment.3 of 17 at approximately the same content level was observed (Figure 1b). The latter was assumed as a marker of the epimerization as the consequence of the process applied in decoction preparation (thermal treatment). Analysis of decoction preparations stored during very long-term period (at least two years) at room temperature in the dark, did not show loss of (−)-EC and (+)-C, nor epimerization progression, thus suggesting very high chemical stability of the preparation. Int. J. Mol. Sci. 2020, 21, x FOR PEER REVIEW 3 of 17 period (at least two years) at room temperature in the dark, did not show loss of (−)-EC and (+)-C, nor epimerization progression, thus suggesting very high chemical stability of the preparation.

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Section: Discussionsupporting

confidence: 78%

Apoptotic-Induced Effects of Acacia Catechu Willd. Extract in Human Colon Cancer Cells

Chiaino

Micucci

Budriesi

et al. 2020

IJMS

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“…Previous studies confirm the benefits of these compounds in animal models of hepatotoxicity. Rudolfová and colleagues shown that catechin administration during DOX therapy seems to be safe and beneficial, since EGCG does not reduce DOX anticancer efficacy while augment hepatocytes viability could ameliorate DOX hepatotoxicity [51]. Another study revealed that vitamin E and catechin can reduce the toxic effects of DOX in liver by ameliorating the action of key enzymes contributing in free radical metabolism, i.e., superoxide dismutase, glutathione peroxidase (GSHPx), catalase (CAT) and malondialdehyde (MDA) in the hepatic tissues of DOX-treated rats.…”

Section: Discussionmentioning

confidence: 99%

Effect of Acacia hydaspica R. Parker extract on lipid peroxidation, antioxidant status, liver function test and histopathology in doxorubicin treated rats

2019

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Background Doxorubicin (DOX) is an anthracycline agent mostly prescribed for various cancers. However, its treatment is contiguous with toxic effects. Acacia hydaspica prevented drug-induced hepatic-toxicity in animals with anti-oxidative mechanisms. We intended to study the efficacy of A. hydaspica ethyl acetate extract (AHE) for inhibiting DOX- induced liver damage. Methods Normal control group received saline; Drug control group received 3 mg/kg b.w. dose of DOX for 6 weeks (single dose/week, intraperitoneal injection) to study the effect of chronic DOX treatment. In co-treatment groups, 200 and 400 mg/kg b.w AHE was given orally for 6 weeks in concomitant with DOX (3 mg/kg b.w, i.p. injection per week). The standard drug group received silyamrin 100 mg/kg b.w (2 doses/week: 12 doses/6 weeks) in conjunction with DOX (single dose/week). Lipid profile, liver function tests (LFTs), antioxidant enzymes, oxidative stress enzymes and morphological alterations were studied to evaluate the hepatoprotective potential of AHE. Results DOX treatment inhibits body weight gain and upturn liver index. DOX considerably upset serum cholesterol, triglycerides and LDL concentration. On the contrary, it reduced serum HDL amount. DOX induced marked depreciation in serum LFTs, diminish hepatic antioxidant enzymes; however, raised tissue oxidative stress markers accompanied by morphological damages. Co-treatment with AHE dose dependently adjusted DOX-prompted fluctuations in lipid profile, AST, ALP, ALT, total bilirubin, and direct bilirubin concentrations and hepatic weight. Likewise, AHE usage enhanced total protein and hepatic tissue antioxidant enzyme quantities whereas declined oxidative stress markers in hepatic tissue. Correspondingly histopathological examinations aid the biochemical results. The influence of AHE 400 mg/kg b.w dose is analogous to silymarin. Conclusion Acacia hydaspica possibly serve as adjuvant therapy that hampers DOX inveigled liver damage due to the underlying antioxidant mechanism of secondary metabolites.

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“…In order to prevent oxidative damage from anticancer drug tegafur, EGCG was applied to exert its antioxidative effect to restore the expression level of α‐defensin resulting in an immunoprotective effect in human intestinal epithelial cells . Additionally, doxorubicin‐induced cardiotoxicity and hepatotoxicity were attenuated by EGCG via decreased production of ROS and increased protein expression of endogenous antioxidant enzymes and ErbB2 …”

Section: Combinational Effects Of Egcg and Other Anticancer Agentsmentioning

confidence: 99%

Recent Advances in Anticancer Activities and Drug Delivery Systems of Tannins

Cai

Zhang

Chen

et al. 2016

Medicinal Research Reviews

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Tannins, polyphenols in medicinal plants, have been divided into two groups of hydrolysable and condensed tannins, including gallotannins, ellagitannins, and (-)-epigallocatechin-3-gallate (EGCG). Potent anticancer activities have been observed in tannins (especially EGCG) with multiple mechanisms, such as apoptosis, cell cycle arrest, and inhibition of invasion and metastases. Furthermore, the combinational effects of tannins and anticancer drugs have been demonstrated in this review, including chemoprotective, chemosensitive, and antagonizing effects accompanying with anticancer effect. However, the applications of tannins have been hindered due to their poor liposolubility, low bioavailability, off-taste, and shorter half-life time in human body, such as EGCG, gallic acid, and ellagic acid. To tackle these obstacles, novel drug delivery systems have been employed to deliver tannins with the aim of improving their applications, such as gelatin nanoparticles, micelles, nanogold, liposomes, and so on. In this review, the chemical characteristics, anticancer properties, and drug delivery systems of tannins were discussed with an attempt to provide a systemic reference to promote the development of tannins as anticancer agents.

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Effect of selected catechins on doxorubicin antiproliferative efficacy and hepatotoxicity in vitro

Cited by 14 publications

References 28 publications

Apoptotic-Induced Effects of Acacia Catechu Willd. Extract in Human Colon Cancer Cells

Apoptotic-Induced Effects of Acacia Catechu Willd. Extract in Human Colon Cancer Cells

Effect of Acacia hydaspica R. Parker extract on lipid peroxidation, antioxidant status, liver function test and histopathology in doxorubicin treated rats

Recent Advances in Anticancer Activities and Drug Delivery Systems of Tannins

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