To cite this paper: Sallam, E.M. and M.M. Anwar, 2017. Antioxidant activity of some extracts from gamma irradiated purslane (Portulaca oleracea) plant.
AbstractPurslane plant is one of the most used medicinal plant listed in the World Health Organization. This paper aims to evaluate the effect of some solvent and different doses of irradiation on antioxidants level and its activity. The extraction of antioxidants was performed by methanol, ethanol and distilled water. Antioxidant activities were determined by Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, ferric reducing power (FRAP) and β-carotene bleaching (βCB). Results indicated that extraction by methanol (50%) had higher total phenolic contents (TPC) than other extracts. As well as, the total antioxidant contents and its activities increased with increasing the irradiation dose. Extraction of irradiated purslane plant at 9 kGy by methanol (50%) considered the effective one to obtained natural phenolic acid compounds; which were identified by HPLC to twenty four components. Moreover, methanol extracts were tested for their antioxidant activity on sunflower oil. The obtained data showed that all tested parameters had higher in comparison to control. Methanol extracts were also tested for their antimicrobial activity, which showed higher inhibition activity of all micro-organism (Pseudomonas aeruginosa, Salmonella typhimurium, Escherichia coli, Staphylococcus aureus, Klebsiella Pneumonia, Bacillus cereus).