Effect of Seed Biopriming on Germination & Seedling Attributes in Chickpea (Cicer arietinum L.)

Abstract

The Study was conducted during Zaid season of 2022-2023 with IPC-05-59 genotype of chickpea (Cicer arietinum L.) Find out the Effect of seed biopriming on germination & seedling attributes in chickpea (Cicer arietinum L). The design of experiment was Completely Randomized Design comprising treatments [viz.T0-uninoculatedT1-Bacillus sp, T2- Mesorhizobium sp., T3- Pseudomonas sp, T4- Bacillus sp(0.5)+ Mesorhizobium sp.(0.5),T5- Pseudomonas sp.(0.5)+ Mesorhizobium sp.(0.5), T6- Bacillus sp.(0.5)+Pseudomonas sp(0.5).+ Mesorhizobium sp.(0.5), T7- Bacillus sp,T8- Mesorhizobium sp, T9- Pseudomonas sp, T10- Bacillus sp.(0.5) + Mesorhizobium sp(0.5),T11-Pseudomonas sp. (0.5)+ Mesorhizobiumsp(0.5),T12- Bacillus sp.(0.5) + Pseudomonas sp. (0.5) + Mesorhizobium sp. (0.5) . The treated seeds along with control were evaluated for their morpho-physiological, growth, yield, root parameters and biochemical parameters under laboratory conditions, The study revealed that seeds biopriming techniques with Bacillus sp.(0.5)+ Pseudomonas sp(0.5).+ Mesorhizobium sp.(0.5) concentration @ 1.0ml for 1 hours T6 recorded significantly higher germination percent (93%), rate of germination (5.38), mean germination time (11.16), root length (11.73cm),shoot length(13.27cm),seedling length(25.00cm), fresh weight (7.91gm),dry weight (1.62gm), vigor index-I (2324.91), vigor index – II (135.82), seed density (1.27), electrical conductivity (0.817), seed metabolic efficiency (1.018). which was followed by seedprimingwithT12- Bacillus sp (0.5) +Pseudomonas sp (0.5) +Mesorhizobium sp (0.5) @ 1.5ml for 2 hours and minimum was recorded in T0 (control).