Effect of Secretin on the Secretion of Acid and Pepsin by the Totally Isolated Canine Stomach

Kolodej²

1977

Self Cite

Totally isolated whole canine stomachs, perfused ex vivo with homologous blood, were used for recording of myoelectrical and mechanical activity prior to and following the injection of secretin. Secretin was given as flash injections (0.5–32 U) or constant infusions (60 U/h). Background stimulation of stomachs was obtained by intragastric instillation of fluid, by the infusion of pentagastrin or bethanechol chloride, and by electrical stimulation of the Latarjet branch of the vagus nerve. Hormones were injected into the gastric arterial circulation. Secretin had no effect on the frequency of electrical control activity. Secretin inhibited electrical response activity when injected against the background of electrical vagus stimulation. Secretin also inhibited or decreased mechanical activity of the gastric antrum, recorded at the site of the antral strain gauge.

Section: Perfused Stomachsmentioning

confidence: 70%

“…1). We used pure synthetic secretin, obtained from Schwarz/Mann (Orangeburg, USA) (19). In a previous study o f isolated canine stomachs, we used 60 U/h o f secretin infused directly into the gastric arterial system.…”

Section: Perfused Stomachsmentioning

confidence: 99%

Effect of Secretin on Myoelectrical and Mechanical Activity of the Isolated Canine Stomach Perfused <i>ex vivo</i>

Kolodej²

1977

Self Cite

“…The perfusion system used in our laboratory for study of the iso lated whole canine stomach has been described previously, and illustrated in detail (10)(11)(12)(13). The stomach is dissected, immediately placed in the perfusion chamber, and connected to the perfusion circuit by way of the preceliac segment of the aorta and the distal portion of the portal vein.…”

Section: Methodsmentioning

confidence: 99%

Effect of Intra-Arterial Infusion of Glucose on Secretory Activity of Isolated Canine Stomach

Kolodej²

1977

Self Cite

Totally isolated stomachs, perfused extracorporeally with homologous blood of living oxygenator dogs, were used for the study of gastric secretion. Pentagastrin or histamine were infused into the gastric artery alone or combined with a 30% solution of glucose. Hyperglycemia was associated with significant inhibition of gastric secretion. Volume, output of HC1 and concentration of H⁺ in gastric secretion were decreased. Hyperglycemia induced also a significant increase in osmotic pressure of gastric secretion and gastric blood. Paired values of H⁺ and osmotic pressure of gastric secretion were analyzed by the method of least squares, using a computer. The regression line was given by the equation: osmotic pressure (mosm/kg) = 311.2 – 0.167 × [H⁺] mEq/liter.

“…We found previously that an identical dose of secretin induced a significant reduction of concentra tion and output of HC1 secretion, and a significant increase of concentration and output of pepsin secre tion, in the isolated canine stomach stimulated by pentagastrin (14). Therefore, we did not repeat the analysis of secretion for HC1 and pepsin, but limited our tests to the determination of mucinous compo nents and amino acids.…”

Section: Analysis O F Gastric Secretionmentioning

confidence: 99%

“…Gastric mucus secretion can be stimulated by secretin, in man (7) and in cats (8,9). Secretin is a known inhibitor of gastric acid secretion and an effective stimulant of gastric pepsin, both in vivo (13) and under ex vivo conditions (14). The effect of secretin on mucus secretion by the ex vivo perfused iso lated canine stomach is not known.…”

mentioning

confidence: 99%

Effect of Secretin on Mucinous Secretion by the Isolated Canine Stomach Perfused Extracorporeally

Pachkowski²,

Kolodej³

1978

Self Cite

Principal glycoproteins, uronic acid and amino acids were determined in canine gastric secretion, obtained from isolated canine stomachs perfused extracorporeally with homologous blood. Gastric secretion was collected during intraarterial infusion of pentagastrin alone for 3 h, and of secretin + pentagastrin for the next 3 h. Secretin produced a significant increase in the output of D-galactose, D-glucosamine, L-fucose, D-glucose, total sugars, total sulfate and uronic acid. Secretin had no significant effect on the output of most of amino acids of gastric secretion.