Effect of Secang Wood (Ceasalpinia Sappan L.) Extract on Morphology of Spermatozoa, Sperm Count, and Reversible Process in Male Rats

Abstract

Background: Limited choice of contraception for males is one of the reasons why their participation is low. Secang wood is considered as an alternative contraception that has an influence in the morphology and number of spermatozoa, and reversible in nature.Objective: To examine the effect of extracts of secang wood in the morphology of spermatozoa, sperm count and reversible process in male mice.Methods: This was a quasy experimental study with randomized post test only control group design. There were 32 male rats (Mus Muscullus L) recruited in this study. Four groups were involved, namely: 1) Ethanol group, 2) Chloroform fraction, 3) Water fraction, and 4) Control group. Each group consisted of 8 mice. There were two treatments in this study: 1) each group was given secang extract with dose 50 mg/25 gram of weight, 2) The observation period was 10 days after the treatment, and 4 mice in each group was dissected to see the morphology and sperm count, while the other 4 mice were allowed to live and maintained until 20 days to see reversible morphology and sperm count Results: The extract of sepang wood at a dose of 50mg/25gram weight could increase the abnormal sperm morphology, and lower sperm count. There was a significant difference between the treatment group and control group with p-value <0.05. The extract was also reversible on the morphology and sperm count.Conclusion: There was a significant effect of secang wood extract on sperm morphology, the number of spermatozoa, and reversible process. This study provides the insight of scientific information about the effect of the sepang extracts on the number and morphology of spermatozoa, and it could be used as a basis for further research in human in the development of natural contraceptive on a reversible man.

Assessment of Teratogenic Effects of Sappan Wood (Caesalpinia Sappan L.) Extract In Rats (Rattus novergicus)

“…In human consumption, Secang wood has been scientifically proven to have numerous medicinal characteristics, and it is frequently drunk by the community as a nutritious beverage. It also contains potent antioxidant extracts with stronger antioxidative indices than commercial antioxidants (Arum et al 2017). In pregnant women, sappan wood extract containing brazilien should be taken with caution.…”

Edukasi Potensi Kayu Secang (Caesalpinia sappan L.) terhadap Kesehatan Reproduksi dengan Metode Webinar

“…Brazilin merupakan komponen yang paling dominan (Wetwitayaklung et al, 2005) dan memberikan warna pada ekstrak kayu secang (Pertamawati & Fahrudin, 2014). Tanaman ini secara empiris dan berdasarkan uji in vitro memiliki efek antifertilitas terhadap sel spermatozoa manusia donor (Arum et al, 2017); dikonsumsi sebagai minuman dendan efek antioksidan (Indah & Tamrin, 2016);digunakan dalam pengobatan diabetes; meningkatkan sirkulasi darah (Xie et al, 2000); dan digunakan sebagai antihiperkolesterolemia, sedasi dan depresi sistem saraf pusat (Hu et al, 2003). Menurut Ayurveda, kayu secang membantu mengobati rasa panas, luka, bisul, kusta, penyakit kulit, diare, dan disentri (SathyaSrilakshmi et al, 2010).…”

“…Adapun dokumentasi kegiatan webinar PkM tersaji pada gambar 2. (Arum et al, 2017), Toxicological studies of Caesalpinia sappan wood derived dye in Wister albino rats (Athinarayanana et al, 2017), Pengaruh Ekstrak Kayu Secang (Caesalpinia sappan L.) terhadap Viabilitas Spermatozoa Mencit Jantan (Mus musculus) (Rusmiati, 2005), dan Reproductive toxicity of brazilein in ICR mice (Yuan et al, 2016).…”