Effect of Seabuckthorn leaves on Antioxidant and Microsomal Enzymes in poultry birds

Abstract

In vivo studies on broiler birds were carried out to evaluate effect of aflatoxin and seabuckthorn leaves on microsomal enzyme system, antioxidant enzymes and biochemical parameters i.e. serum triglyceride, total plasma protein, aminopyrine demethylase, aniline hydroxylase, NADPH cytochrome P450 reductase, catalase, LPO, superoxide dismutase, GSH, blood urea nitrogen (BUN) and creatinine levels in poultry. The poultry birds were divided into six groups containing six birds each. Aflatoxin (400 ppb) and seabuckthorn leaves (10000ppm) was administered continuously in poultry feed. Aflatoxin increased serum triglyceride, blood urea nitrogen and creatinine levels where as seabuckthorn leaves supplementation at 10000ppm significantly decreased triglyceride (P<0.05), blood urea nitrogen (P<0.05) and creatinine levels in birds. Toxin decreased liver, kidney and blood superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) activity, whereas, seabuckthorn leaves (SBTL) increased the activity of these enzymes as compared to control group. The level of lipid peroxidation was significantly increased in the toxin exposed group and decreased in case of SBTL. The activity of Aminopyrine demethylase and Aniline hydroxylase increased, while the activity of NADPH cytochrome P450 reductase is decreased in case of toxin group whereas in case of seabuckthorn leaves exposed group showed no significant change in case of aminopyrine demethylase and NADPH cytochrome P450 reductase, however, the activity of Aniline hydroxylase decreased. On the basis of present study, it could be concluded that the seabuckthorn leaves reduced the effect of Aflatoxin which produced oxidative stress by altering the levels of antioxidant enzymes of liver and kidney in adult poultry birds

Comparative Effects on Using Bilberry Leaves in Broiler Diet Reared under Thermoneutral Conditions vs. Heat Stress on Performance, Health Status and Gut Microbiota

“…Bilberry leaves are also being studied for their potential benefits in broiler diets due to their high content of polyphenols, zinc, vitamin E, lutein, and zeaxanthin, as well as their antioxidant capacity [6]. These compounds have biological and therapeutic activities such as antioxidant, anti-stress and immunomodulatory properties [7,8]. Some authors [9] reported that the dietary bilberry leaves positively influence the microbiota of laying hens, while others [10] showed that bilberry leaves increased the total polyphenol content, and the zinc, lutein and zeaxanthin concentrations in the egg yolks.…”