Effect of Sargassum fusiforme polysaccharide on apoptosis and its possible mechanism in human erythroleukemia cells

Ding, Haomiao; Chen, Xue-Jia; Chen, Haimin; Wang, Caisheng; Qian, Guo‐Ying

doi:10.1016/s1875-5364(20)60015-2

Cited by 9 publications

(7 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To further confirm that GLP5 induced apoptosis in Jurkat cells, we analyzed the expression of several key genes involved in apoptosis pathways and found that proapoptotic genes that cleaved caspase-3 and BAX were upregulated and anti-apoptotic gene Bcl-2 was down-regulated after the Jurkat cells were exposed to GLP5, indicating that GLP5 has an impact on apoptosis pathways. These results are consistent with the previous studies with lentinan on HL-60 cells (Ma et al, 2012), Sargassum fusiforme polysaccharide on human erythroleukemia cells (Ding et al, 2020) and pectin-like polysaccharide from Polygala tenuifolia on pancreatic cancer cells (Bian et al, 2020). Caspase-3 is the best recognized biochemical hallmark of both early and late stages of apoptosis and ex-ists in the form of pro-caspase-3.…”

Section: Discussionsupporting

confidence: 92%

Ganoderma lucidum polysaccharide inhibits the proliferation of leukemic cells through apoptosis

Zhong

Huang

Mao³

et al. 2022

Acta Biochim Pol

View full text Add to dashboard Cite

Objective: To investigate the cytotoxic effect of polysaccharides derived from Ganoderma lucidum on T lymphocyte leukemia cells. Methods: Water-soluble polysaccharides were extracted from the fruit bodies of G. lucidum, purified, and characterized using HPGPC-MALLS and NMR. The cytotoxicity of G. lucidum polysaccharide fraction 5 (GLP5) to T lymphocyte leukemia cell line Jurkat and human immortalized epidermal cell line HaCat was assessed using MTT assay. Apoptosis was assessed using flow cytometry. Expressions of apoptosis-related genes in the cells after being exposed to GLP5 were detected using Western blot assay.Results: GLP5 was a β-(1→3) and β-(1→6) linked glucan. It inhibited the proliferation of Jurkat cells in a concentration-dependent manner and the half-maximal inhibitory concentration (IC50) was 34.5 mg/L but did not suppress the growth of HaCat cells. Apoptotic cells in Jurkat cells were detected to increase with increasing GLP5 concentrations. The expression levels of cleaved caspase-3 were significantly higher after the cells were exposed to 25 and 50 mg/L GLP5 when compared to non-exposed cells (Control). In addition, the expression levels of BAX and Bcl2 were significantly up- and down-regulated after treatment with GLP5 at 25 and 50 mg/L when compared with control (P<0.05), respectively.Conclusions: GLP5 has antiproliferative activity against Jurkat cells and the activity is likely mediated through the activation of apoptosis pathways.

show abstract

Section: Discussionsupporting

confidence: 92%

Ganoderma lucidum polysaccharide inhibits the proliferation of leukemic cells through apoptosis

Zhong

Huang

Mao³

et al. 2022

Acta Biochim Pol

View full text Add to dashboard Cite

show abstract

“…The results showed that it decreased the viability of HEL cells and induced apoptosis of HEL cells. The apoptosis induction mechanism was found to be related to the formation of ROS, which in turn can lead to DNA damage and cell death, associated with induction of cell cycle arrest in the G 0 / G 1 phase, and increased expression of apoptosisrelated genes and proteins (20) .…”

Section: Table 1 Effect Of Fucoidan Phloroglucinol and Fucoxanthin Onapoptosismentioning

confidence: 99%

The Effect of Brown Seaweed (Sargassum sp.) Extract on Apoptosis Process in Breast Cancer – A Literature Review

Ismylatifa Devi,

Diah Purwaningsari,

Nita Pranitasari

et al. 2021

Indian Journal of Forensic Medicine & Toxicology

View full text Add to dashboard Cite

Breast cancer was one of the most common types of cancer in women and the major cause of death. Chemotherapy in breast cancer caused toxicity to the normal cells, resistance to the drugs, and several other side effects. Seaweed had many functional compounds that could be used as adjuvant therapy in breast cancer because of its anti-cancer properties called the proapoptotic agent. These compounds were fucoidan, phloroglucinol, and fucoxanthin. Their proapoptotic mechanisms shown by the journal were through increasing proapoptotic protein expression, decreasing antiapoptotic protein expression, inducing oxidative stress production on the cancer cell, and inhibiting the PI3K pathway. This review concludes that the brown seaweed (Sargassum sp.), which contains fucoidan, phloroglucinol, and fucoxanthin, could increase breast cancer cells' apoptotic process.

show abstract

“…Morchella esculenta polysaccharide had a protective effect on dextran-sodium-sulfate-induced liver injury, and its mechanism may be related to reducing oxidative stress, inhibiting inflammatory response and increasing the activity of liver antioxidant enzymes [ 10 ]. Agents such as Sargassum fusiforme polysaccharides can effectively suppress cancer cell proliferation and stimulate apoptosis [ 11 ]. The biological activity of polysaccharides is directly tied to their structure.…”

Section: Introductionmentioning

confidence: 99%

“…Caspases have become targets for designing new antitumoral drugs and treatments, and several studies have reported that natural components exhibit mechanisms with antitumoral activity to activate the caspase signaling pathway. Our prior study showed that S. fusiforme polysaccharide significantly enhanced Bax, Bad, and Caspase-3 expression in human erythroleukemia (HEL) cells via the caspase signaling pathway [ 11 ]. However, whether S. fusiforme polysaccharide stimulates HEL cell apoptosis via the caspase signaling pathway in vivo and plays an anti-erythroleukemia role remains unknown.…”

Section: Introductionmentioning

confidence: 99%

Anti-Leukemia Activity of Polysaccharide from Sargassum fusiforme via the PI3K/AKT/BAD Pathway In Vivo and In Vitro

Jin

et al. 2023

Marine Drugs

View full text Add to dashboard Cite

Studies have shown that Sargassum fusiforme and its extracts are effective herbal treatments for leukemia. We previously found that a polysaccharide from Sargassum fusiforme, SFP 2205, stimulated apoptosis in human erythroleukemia (HEL) cells. However, the structural characterization and antitumoral mechanisms of SFP 2205 remain uncertain. Here, we studied the structural characteristics and anticancer mechanisms of SFP 2205 in HEL cells and a xenograft mouse model. The results demonstrated that SFP 2205, with a molecular weight of 41.85 kDa, consists of mannose, rhamnose, galactose, xylose, glucose, and fucose with monosaccharides composition of 14.2%, 9.4%, 11.8%, 13.7%, 11.0%, and 38.3%, respectively. On animal assays, SFP 2205 significantly inhibited growth of HEL tumor xenografts with no discernible toxicity to normal tissues. Western blotting showed that SFP 2205 therapy improved Bad, Caspase-9, and Caspase-3 protein expression, and ultimately induced HEL tumor apoptosis, indicating mitochondrial pathway involvement. Furthermore, SFP 2205 blocked the PI3K/AKT signaling pathway and 740 Y-P, an activator of the PI3K/AKT pathway, rescued the effects of SFP 2205 on HEL cell proliferation and apoptosis. Overall, SFP 2205 may be a potential functional food additive or adjuvant for preventing or treating leukemia.

show abstract

Effect of Sargassum fusiforme polysaccharide on apoptosis and its possible mechanism in human erythroleukemia cells

Cited by 9 publications

References 52 publications

Ganoderma lucidum polysaccharide inhibits the proliferation of leukemic cells through apoptosis

Ganoderma lucidum polysaccharide inhibits the proliferation of leukemic cells through apoptosis

The Effect of Brown Seaweed (Sargassum sp.) Extract on Apoptosis Process in Breast Cancer – A Literature Review

Anti-Leukemia Activity of Polysaccharide from Sargassum fusiforme via the PI3K/AKT/BAD Pathway In Vivo and In Vitro

Contact Info

Product

Resources

About