Effect of sampling method and incubation temperature on fungal culture in canine sinonasal aspergillosis

Billen, Frédéric; Clercx, Cécile; Garérrès, A. Le; Massart, L; Mignon, Bernard; Peeters, Dominique

doi:10.1111/j.1748-5827.2008.00672.x

Cited by 16 publications

(19 citation statements)

References 22 publications

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“…The causative agents and the breeds being affected are similar to those seen in the nasal form of aspergillosis (1,5,8,25). The clinical signs are nonspecific, including depression, fever, and cough (1,5,25). Cytologic evaluation of the bronchoalveolar lavage fluid often reveals a mixed inflammatory response dominated by neutrophils and macrophages but rarely reveals the presence of fungal elements (5).…”

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confidence: 79%

“…Bronchopulmonary aspergillosis is a rare disease in dogs (1,40,44). The causative agents and the breeds being affected are similar to those seen in the nasal form of aspergillosis (1,5,8,25). The clinical signs are nonspecific, including depression, fever, and cough (1,5,25).…”

mentioning

confidence: 99%

“…The clinical signs are nonspecific, including depression, fever, and cough (1,5,25). Cytologic evaluation of the bronchoalveolar lavage fluid often reveals a mixed inflammatory response dominated by neutrophils and macrophages but rarely reveals the presence of fungal elements (5). Chest radiographs can demonstrate diffuse nodular lesions in the lung.…”

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Aspergillus versicolor, a New Causative Agent of Canine Disseminated Aspergillosis

et al. 2012

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Disseminated aspergillosis in dogs has been associated with Aspergillus terreus or A. deflectus infection. We report a case of disseminated A. versicolor infection presenting as diskospondylitis, osteomyelitis, and pyelonephritis. The diagnosis was made based on clinical, radiographic, and pathological findings. The etiologic agent was identified by fungal culture and internal transcribed spacer (ITS) ribosomal DNA (rDNA) sequencing. This is the first description of canine aspergillosis caused by A. versicolor. CASE REPORTA 31-kg, 2.5-year-old male castrated German shepherd dog was examined at the Texas A&M University Veterinary Medicine Teaching Hospital because of nonambulatory paraparesis, weight loss, and hyporexia for 3 months. At admission, the dog had a body temperature of 104.6°F, heart rate of 160 beats/min, respiratory rate of 80 breaths/min, and blood pressure of 173/99 mm Hg. Neurological examination revealed a hyperreflexive patellar reflex bilaterally. Motor function was absent in the right pelvic limb and questionable in the left. The patient had marked generalized muscle atrophy. A lateral radiograph of the cranial thorax revealed lysis and shortening of the first four sternebrae (Fig. 1A). The second and third sternebrae were most severely affected and had irregular margins with loss of their end plates. Lateral radiographs of the thoracic vertebral column showed lysis and shortening of the 9th (T9) and 10th (T10) thoracic vertebrae with loss of the end plates and spondylosis deformans ventrally (Fig. 1B). Narrowing of the intervertebral space, end plate sclerosis, and ventral spondylosis derformans were also found between the seventh and eighth thoracic vertebrae. The clinical diagnoses were diskospondylitis involving T9-T10, osteomyelitis of the sternum and left humerus, and T3-L3 myelopathy resulting in nonambulatory paraparesis. Suspected causes included disseminated aspergillosis, blastomycosis, coccidioidomycosis, bacterial infection, and neoplasia. Due to the poor prognosis, the dog was subsequently euthanized and a complete necropsy was performed.The major skeletal changes found at postmortem examination included marked bony proliferation of the cranial end of the sternum, extending from the first to the fourth sternebrae, with loss of the joint space between the second and third sternebrae ( Fig. 2A). A soft gray area of necrotizing osteomyelitis was in the center of the collapsed and fused sternebrae. In the thoracic vertebral column, there was loss of the intervertebral disk at T9-T10 with lysis of the associated vertebral end plates (Fig. 2B). The latter changes resulted in joint instability, overriding of the vertebral bodies, and spinal cord compression that was exacerbated with ventroflexion of the vertebral column. In the kidneys, there was dark red to purple mottling of the cortical region with dozens of white to tan areas throughout the cortex and medulla (Fig. 2C). The renal crests were ulcerated, and the pelvises were dilated and contained a small amount of cloudy fluid with ...

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Aspergillus versicolor, a New Causative Agent of Canine Disseminated Aspergillosis

et al. 2012

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“…Aspergilus flavus) may be found as endogenous flora in the nasal cavity in many dogs (Sharp et al 1991, Mathews 2004, Peeters and Clercx 2007, Żmudzka and Lechowski 2008. A recent study (Billen et al 2009) suggests better sensitivity of fungal culture when samples are taken under visual control (endoscopy) and incubated in temperatures of 37 centigrades. However, results of mycology are available after a few days and may be ambiguous.…”

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confidence: 99%

“…The most important differential diagnosis for fungal rhinitis is nasal neoplasia, so careful diagnostic evaluation should be performed in all cases (Johnson et al 2006). Several diagnostic techniques can be used to confirm the diagnosis of fungal rhinitis, including radiography, computed tomography, magnetic resonance imaging, rhinoscopy and serology (Saunders and van Bree 2003, De Lorenzi et al 2006, Johnson et al 2006, Pomrantz et al 2007, Billen et al 2009). It should be underlined that fungal rhinitis is not always a primary disease and could be secondary to immunosuppression, foreign body presence, and rarely to neoplastic process.…”

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confidence: 99%

Fungal rhinitis in dogs

Ostrzeszewicz¹,

Sapierzyński²

2015

Polish Journal of Veterinary Sciences

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Fungal rhinitis and sinusitis in dogs are quite common reasons of chronic nasal discharge and rhinoscopy in such cases is commonly suggested. Forty three dogs were examined using rhinoscopy because of the presence of chronic airway symptoms. Clinical examination, routine hematology and serum biochemistry profiles, nasal and frontal sinus radiographs were made in all animals. Additionally, computed tomography in one dog was performed. Samples for histopathology were taken from 9 patients during rhinoscopy, additionally, from 8 of these patients samples for cytopathology were collected by blind nasal swab technique. In 9 of 43 dogs (20,5%), all males aged 1 to 13 years, examinations led to a diagnosis of fungal rhinitis. In 2 cases a diagnosis of fungal rhinitis was obtained based solely on cytopathology, while in 7 cases -mycosis of nasal mucosa was confirmed by histopathology. The present study revealed that cytopathological examination of nasal swabs has a low diagnostic value in the case of nasal infections in dogs. Although, in some dogs cytopathology, together with other widely available diagnostic techniques was sufficient to reliably diagnose fungal rhinitis, histopathology of samples collected during rhinoscopy is still the gold standard in such cases.

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Aspergillosis and Penicilliosis

Barrs¹,

Dear²

2021

Greene's Infectious Diseases of the Dog and Cat

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Effect of sampling method and incubation temperature on fungal culture in canine sinonasal aspergillosis

Cited by 16 publications

References 22 publications

Aspergillus versicolor, a New Causative Agent of Canine Disseminated Aspergillosis

Aspergillus versicolor, a New Causative Agent of Canine Disseminated Aspergillosis

Fungal rhinitis in dogs

Aspergillosis and Penicilliosis

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